Recoverin depletion accelerates cone photoresponse recovery

Zang, Jingjing; Keim, Jennifer; Kastenhuber, Edda; Gesemann, Matthias

doi:10.1098/rsob.150086

Cited by 36 publications

(44 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even more strikingly, we found the UV-sensitive opsin 1 (cone pigments), short-wave-sensitive 1 (opn1sw1) to be expressed in bsx mutants, whereas it was not expressed in wild-type PGs in the larval stages. However, when we assessed the expression of rcv1a, which is specific to UV cones and rods (Zang et al, 2015), we observed a significant downregulation in bsx mutants. This result suggests that rod-like PhR cells are also affected in bsx mutants.…”

Section: Bsx Position In the Pineal Complex Transcriptional Networkmentioning

confidence: 79%

Bsx controls pineal complex development

Schredelseker

Driever

2018

Development

View full text Add to dashboard Cite

Neuroendocrine cells in the pineal gland release melatonin during the night and, in teleosts, are directly photoreceptive. During development of the pineal complex, a small number of cells migrate leftward away from the pineal anlage to form the parapineal cell cluster, a process that is crucial for asymmetrical development of the bilateral habenular nuclei. Here, we show that, throughout zebrafish embryonic development, the () gene is expressed in all cell types of the pineal complex. We identified Bmp and Noto/Flh as major regulators of expression in the pineal complex. Upon loss of Bsx through the generation of a targeted mutation, embryos fail to form a parapineal organ and develop right-isomerized habenulae. Crucial enzymes in the melatonin biosynthesis pathway are not expressed, suggesting the absence of melatonin from the pineal gland in mutants. Several genes involved in rod-like or cone-like phototransduction are also abnormally expressed, indicating that Bsx has a pivotal role in the differentiation of multiple cell types in the zebrafish pineal complex.

show abstract

Section: Bsx Position In the Pineal Complex Transcriptional Networkmentioning

confidence: 79%

Bsx controls pineal complex development

Schredelseker

Driever

2018

Development

View full text Add to dashboard Cite

show abstract

“…6g). Already the modulation of single gene products' relative expression levels could have a major effect, most notably in the case of transducin and GC3, and to a lesser extent also the downregulation of recoverin 2 (Rec2 (Dizhoor et al, 1991;Zang et al, 2015)) ( Fig. 6h).…”

Section: Differentialmentioning

confidence: 99%

Cellular and molecular mechanisms of photoreceptor tuning for prey capture in larval zebrafish

Yoshimatsu

Schröder

et al. 2019

Preprint

View full text Add to dashboard Cite

Correspondence to t. yoshimatsu@sussex.ac.uk and t.baden@sussex.ac.uk In the eye, the function of same-type photoreceptors must be regionally adjusted to process a highly asymmetrical natural visual world. Here we show that UV-cones in the larval zebrafish area temporalis are specifically tuned for UV-bright prey capture in their upper frontal visual field, which uses the signal from a single cone at a time. For this, UV-detection efficiency is regionally boosted 42-fold. Next, in vivo 2-photon imaging, transcriptomics and computational modelling reveal that these cones use an elevated baseline of synaptic calcium to facilitate the encoding of bright objects, which in turn results from expressional tuning of phototransduction genes. Finally, this signal is further accentuated at the level of glutamate release driving retinal networks. These regional differences tally with variations between peripheral and foveal cones in primates and hint at a common mechanistic origin. Together, our results highlight a rich mechanistic toolkit for the tuning of neurons.

show abstract

“…The set of eye genes also included genes coding for proteins involved in the phototransduction cascade: RPE65, Arrestins, Recoverins, Transducins, PDE6, CNGA3 and CNGB3, GCAPs, zGCs, and GRKs. These genes code for a highly heterogeneous set of proteins with regard to their structure and functions (Imanishi, et al 2002; Wada, et al 2006; Schonthaler, et al 2007; Matveev, et al 2008; Nishiwaki, et al 2008; Rätscho, et al 2009; Renninger, et al 2011; Fries, et al 2013; Lagman, et al 2015; Zang, et al 2015; Lagman, et al 2016). Only genes whose expression was restricted to the retina and/or the pineal complex were retained.…”

Section: Methodsmentioning

confidence: 99%