Abstract:This unit describes techniques for the preparation of mouse cerebellar slices and electrophysiological recording from neurons in the slice. The cerebellum provides a model of motor learning that can be correlated with alterations in synaptic function. In addition, the architecture and well-defined synaptic pathways in the cerebellar slice make this preparation a useful model for exploring general principles of synaptic transmission.
“…Stimulation intensity was then adjusted to evoke the first EPSC of approximately 150 pA to record a baseline for synaptic plasticity experiments. In all cells, baseline EPSCs were then evoked for 5 to 10 min with paired-pulse stimuli (60-ms interpulse interval) at a frequency of 0.067 Hz (57). The paired-pulse ratio (EPSC 2 /EPSC 1 ) was used to monitor changes in presynaptic function during LTD experiments.…”
“…Stimulation intensity was then adjusted to evoke the first EPSC of approximately 150 pA to record a baseline for synaptic plasticity experiments. In all cells, baseline EPSCs were then evoked for 5 to 10 min with paired-pulse stimuli (60-ms interpulse interval) at a frequency of 0.067 Hz (57). The paired-pulse ratio (EPSC 2 /EPSC 1 ) was used to monitor changes in presynaptic function during LTD experiments.…”
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