Record of<i>Phlebotomus (Transphlebotomus) mascittii</i>Grassi, 1908 and<i>Phlebotomus (Larroussius) chadlii</i>Rioux, Juminer &amp; Gibily, 1966 female in Algeria

Berdjane-Brouk, Zohra; Charrel, Rémi N.; Bitam, Idir; Hamrioui, B; Izri, Arezki

doi:10.1051/parasite/2011184337

Cited by 22 publications

(14 citation statements)

References 6 publications

(3 reference statements)

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“…In the present study, nearly 30% of the specimens which were caught and frozen were submitted to MALDI-TOF MS for identification. Of the 24 recently described in Algeria [ 25 , 26 ], six sand fly species were found at the four prospected sites. These phlebotomine species were described as Leishmania vectors [ 4 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

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Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

et al. 2016

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BackgroundPhlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. Precise sand fly identification is essential to prevent phlebotomine-borne diseases. Over the past two decades, progress in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an accurate tool for arthropod identification. The objective of the present study was to investigate the usefulness of MALDI-TOF MS as a tool for identifying field-caught phlebotomine.Methodology/Principal FindingsSand flies were captured in four sites in north Algeria. A subset was morphologically and genetically identified. Six species were found in these areas and a total of 28 stored frozen specimens were used for the creation of the reference spectrum database. The relevance of this original method for sand fly identification was validated by two successive blind tests including the morphological identification of 80 new specimens which were stored at -80°C, and 292 unknown specimens, including engorged specimens, which were preserved under different conditions. Intra-species reproducibility and inter-species specificity of the protein profiles were obtained, allowing us to distinguish specimens at the gender level. Querying of the sand fly database using the MS spectra from the blind test groups revealed concordant results between morphological and MALDI-TOF MS identification. However, MS identification results were less efficient for specimens which were engorged or stored in alcohol. Identification of 362 phlebotomine sand flies, captured at four Algerian sites, by MALDI-TOF MS, revealed that the subgenus Larroussius was predominant at all the study sites, except for in M’sila where P. (Phlebotomus) papatasi was the only sand fly species detected.ConclusionThe present study highlights the application of MALDI-TOF MS for monitoring sand fly fauna captured in the field. The low cost, reliability and rapidity of MALDI-TOF MS analyses opens up new ways in the management of phlebotomine sand fly-borne diseases.

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Section: Discussionmentioning

confidence: 99%

“…In Algeria, 24 phlebotomine sand flies species were inventoried [ 25 , 26 ], including two genera and seven subgenera [ 27 ]. Some of them are known to be Leishmania parasite vectors [ 27 – 29 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

et al. 2016

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“…Recently, two molecular studies characterised two closely related species ( Phlebotomus chabaudi and Phlebotomus riouxi ) having undistinguishable or very difficultly distinguishable females from North Africa (Bounamous et al 2008, Boudabous et al 2009) and a new species for the country (and for Africa) has been recorded: Phlebotomus mascittii Grassi (Berdjane-Brouk et al 2011). …”

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Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism

Bounamous

Lehrter

Hadj-Henni

et al. 2014

Mem. Inst. Oswaldo Cruz

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A total of 131 phlebotomine Algerian sandflies have been processed in the present study. They belong to the species Phlebotomus bergeroti, Phlebotomus alexandri, Phlebotomus sergenti, Phlebotomus chabaudi, Phlebotomus riouxi, Phlebotomus perniciosus, Phlebotomus longicuspis, Phlebotomus perfiliewi, Phlebotomus ariasi, Phlebotomus chadlii, Sergentomyia fallax, Sergentomyia minuta, Sergentomyia antennata, Sergentomyia schwetzi, Sergentomyia clydei, Sergentomyia christophersi and Grassomyia dreyfussi. They have been characterised by sequencing of a part of the cytochrome b (cyt b), t RNA serine and NADH1 on the one hand and of the cytochrome C oxidase I of the mitochondrial DNA (mtDNA) on the other hand. Our study highlights two sympatric populations within P. sergenti in the area of its type-locality and new haplotypes of P. perniciosus and P. longicuspis without recording the specimens called lcx previously found in North Africa. We tried to use a polymerase chain reaction-restriction fragment length polymorphism method based on a combined double digestion of each marker. These method is not interesting to identify sandflies all over the Mediterranean Basin.

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“…mascittii has a large range of distribution and it is present throughout most European countries of the Mediterranean basin [9] as well as adjacent areas north of this region, including sporadic findings in Belgium [4], Germany [13, 15], Austria [16] and Hungary [6]. A recent single record in Algeria also suggests its occurrence in North Africa [2]. Other species of the subgenus Transphlebotomus seem to have markedly more restricted distribution.…”

Section: Discussionmentioning

confidence: 99%

First record ofPhlebotomus (Transphlebotomus) mascittiiin Slovakia

et al. 2016

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Record ofPhlebotomus (Transphlebotomus) mascittiiGrassi, 1908 andPhlebotomus (Larroussius) chadliiRioux, Juminer & Gibily, 1966 female in Algeria

Abstract: We report for the first time the presence of Phlebotomus mascittii and the female of Phlebotomus chadlii in Algeria. These two species were collected during an entomological study conducted in endemic visceral leishmaniasis focus from the north part of the country, Kabylia.

Cited by 22 publications

References 6 publications

Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism

First record ofPhlebotomus (Transphlebotomus) mascittiiin Slovakia

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