Reconstructive Urethroplasty Using Porcine Acellular Matrix

Mantovani, F.; Trinchieri, Alberto; Castelnuovo, Chiara; Romanò, Ai Ling; Pisani, E.

doi:10.1016/s0302-2838(03)00212-4

Cited by 75 publications

(39 citation statements)

References 6 publications

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“…Many organs, e.g. urinary bladder [6], small intestinal [7][8][9][10][11], heart valves [12][13][14][15], skin [16,17], liver [18], anterior crucial ligament bone [19], kidney [20], pericardium [21], blood vessels [22], nerves [23], skeletal muscle [24], tendon [25] and vocal cord [26,27] from bovine and porcine tissues, have been used for fabricating the ECMbased scaffold. But, the use of goat-lung has not been focused for tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Gupta¹,

Dinda²,

Mishra³

2017

Biol Eng Med

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In this study, scaffold was fabricated from goat-lung of cadaver goat by decellularization method for its potential application in tissue engineering. The cellular components of goat-lung were removed by employing trypsin-SDS based method of decellularization. DNA quantification and DAPI staining verified the effective removal of native cells from the goat-lung. Verhoeff-Van Gieson (VVG) staining, H&E staining and Periodic-Acid Schiff (PAS) staining histologically assessed the composition of native and decellularized goat-lung matrix-demonstrating the presence of collagen, elastin and glycoproteins in the decellularized goat-lung matrix. Antibacterial activity of decellularized goat-lung matrix was evaluated against gram-negative (Escherichiacoli) and gram-positive (Staphylococcusaureus) bacteria, which shows the presence of naturally occurring bioactive peptides-exhibiting the antibacterial activity against E. coli for up to 9h and against S. aureus for up to 5h. The decellularized goat-lung matrix, as demonstrated by MTT assay, was found to be biocompatibile to L929 mouse fibroblasts, HepG2, human skin derived mesenchymal stem cells (hS-MSCs), osteoblasts and BMMSCs. All the above results confer the applicability of the decellularized goat-lung matrix as a potential scaffold for tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Gupta¹,

Dinda²,

Mishra³

2017

Biol Eng Med

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“…[1][2][3][4][5][6][7][8][9][10][11][12] Such biological scaffolds have been surprisingly resistant to bacterial infection, [13][14][15][16] even in clinical applications that are at high risk for bacterial contamination. [17][18][19][20] Generally, naturally occurring biomaterials such as those composed of purified collagen or intact ECM are more resistant to bacterial infection than synthetic biomaterials. [13][14][15][16]21 Porcine-derived ECM composed of small intestinal sub-mucosa (SIS) has been successfully used as a resorbable biological scaffold for tissue-engineering applications in more than 500,000 human patients and has shown resistance to deliberate bacterial infection in preclinical studies.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Brennan

Reing²,

Chew³

et al. 2006

Tissue Engineering

209

133

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Biological scaffolds composed of extracellular matrix (ECM) have been shown to be resistant to deliberate bacterial contamination in preclinical in vivo studies. The present study evaluated the degradation products resulting from the acid digestion of ECM scaffolds for antibacterial effects against clinical strains of Staphylococcus aureus and Escherichia coli. The ECM scaffolds were derived from porcine urinary bladder (UBM-ECM) and liver (L-ECM). These biological scaffolds were digested with acid at high temperatures, fractionated using ammonium sulfate precipitation, and tested for antibacterial activity in a standardized in vitro assay. Degradation products from both UBM-ECM and L-ECM demonstrated antibacterial activity against both S. aureus and E. coli. Specific ammonium sulfate fractions that showed antimicrobial activity varied for the 2 different ECM scaffold types. The results of this study suggest that several different lowmolecular-weight peptides with antibacterial activity exist within ECM and that these peptides may help explain the resistance to bacterial infection provided by such biological scaffolds.

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“…Most reports have been focused on treating urethral strictures using SIS. Among them, most results were satisfactory (Table 2) (50)(51)(52)(53)(54)(55)(56). In the report of Fiala et al, fifty patients with urethral strictures received urethroplasty using SIS.…”

Section: Clinical Applicationmentioning

confidence: 94%