Reconstructive and Stabilizing Ability of the Maize Lines in Sterile Cytoplasm “M” and “C” Types of CMS

Abstract

Seeds of maize hybrids in the Russian Federation are obtained using CMS, therefore it is necessary to transfer the developed hybrids to a sterile basis to introduce them into production. The purpose of the work is to study the reaction of self-pollinated maize lines to various types of CMS. There was estimated the response of 26 self-pollinated maize lines to the “M” type of CMS and the response of 23 lines to the “C” type of CMS. The simple sterile hybrids “Milena M”, “Madonna M”, “Krucha M”, “Istok C”, “Nimfa C”, “Malvina C”, which are the mother forms of the new three-linear maize hybrids have been used as the testers of the sources of sterility. The self-pollinated lines have been classified according to the response to the “M” type of CMS as sterility fixers (11), incomplete sterility fixers (7), fertility reducing agents (6), non-uniform in reconstructing ability (2). In relation to the “C” type of CMS there were identified 10 sterility fixers, 3 incomplete sterility fixers, 7 fertility reducing agents, 3 non-uniform in reconstructing ability. The reducing agents of Moldavian type of CMS “FC 18 A”, “T 22 A”, “DS 768/83-3”, “DS 768/85-4”, “DS 768/85”, “DS 768/85-5” are of the greatest practical interest as they can be used as natural reducing agents (paternal forms) in the developed hybrid combinations. The constant natural reducing agents of the “C” type of CMS (“T 22 A”, “PLS 61”, “DK 22/325”, as paternal forms of hybrids grown on sterile cytoplasm of the “C” type) are of practical value. The use of sterile testers in hybridization allows simultaneously with the development of new hybrids to start the first stage of their transfer to a sterile basis, namely to evaluate the response of the lines to the sterile cytoplasm.