Reconstruction of limbal stem cell deficient corneal surface with induced human bone marrow mesenchymal stem cells on amniotic membrane

Rohaina, C.M.; Then, K.; Ng, Angela Min Hwei; Halim, Wan Haslina Wan Abdul; Zahidin, Aida Zairani Mohd; Saim, Aminuddin Bin; Idrus, Ruszymah Bt Hj

doi:10.1016/j.trsl.2013.11.004

Cited by 81 publications

(85 citation statements)

References 31 publications

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“…Besides the above-cited examples, reports of corneal epithelial cell culture and CLET have come from various countries across the globe, including Brazil [59], Iran [60], India [61], Thailand [62], and Malaysia [45] (just to name those published in the public domain). The ease with which CLET can be successfully simplified and used as SLET makes this form of stem cell therapy available to qualified corneal surgeons in centers and countries without the availability of or, indeed, the need for laboratory culture facilities.…”

Section: Resultsmentioning

confidence: 99%

“…Cells derived this way were cultured on hAM and transplanted in nude rats with corneas that were alkali injured. Corneal healing was observed after 8 weeks [45]. Similarly, a Brazilian group has used immature dental pulp stem cells and cultured them on a temperature-responsive cell culture dish using a slightly modified Dulbecco's modified Eagle's medium: Nutrient Mixture F-12 to generate a cell sheet.…”

Section: Other Cells As Substitutesmentioning

confidence: 99%

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Concise Review: The Coming of Age of Stem Cell Treatment for Corneal Surface Damage

Ramachandran

Basu

Sangwan

et al. 2014

Stem Cells Translational Medicine

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The cornea is a vital component of the eye because it provides approximately 70% of the refraction and focusing of incoming light. Being the outermost surface of the eye, it faces continuous stress from dryness, photodamage, infection, and injury; however, like the skin, the cornea regularly refreshes itself by shedding its epithelial cells, which are readily replaced, keeping the ocular surface stable and functional. This regular turnover of the corneal epithelial cells occurs through the stem cells in the limbus, an annular ring of a tissue surrounding the cornea, separating it from the sclera and the conjunctival membrane. The loss of this reserve of stem cells leads to a condition called limbal stem cell deficiency. Treatment for this disorder has evolved from transplanting whole limbal tissues to the affected eye to transplanting laboratory cultured limbal cells. This procedure is called cultivated limbal epithelial transplantation (CLET). Since its start in 1997, more than 1,000 CLET procedures have been reported from around the world, with varying degrees of success. In this paper, we compare the methods of cultivation and the outcomes and discuss some problem areas, use of other cells as substitutes for limbal epithelium, and various carrier materials used in transplantation. Our analysis suggests that CLET as a treatment for corneal surface damage has come of age. We also highlight a simpler procedure (simple limbal epithelial transplantation) that involves cultivation of limbal tissue in situ on the surface of the cornea in vivo and that has outcomes comparable to CLET.

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Section: Resultsmentioning

confidence: 99%

Section: Other Cells As Substitutesmentioning

confidence: 99%

Concise Review: The Coming of Age of Stem Cell Treatment for Corneal Surface Damage

Ramachandran

Basu

Sangwan

et al. 2014

Stem Cells Translational Medicine

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“…Non-LSCs, such as mesenchymal stem cells have been suggested to be capable of reconstructing a corneal epithelial surface in animal model of LSCD. 27 It has been shown that mesenchymal cells can serve as feeder cells in vitro for the expansion of LSCs. 28,29 These mesenchymal cells provide factors for the successful propagation of LSCs.…”

Section: Discussionmentioning

confidence: 99%

Existence of Normal Limbal Epithelium in Eyes With Clinical Signs of Total Limbal Stem Cell Deficiency

Chan

Codriansky

et al. 2016

Cornea

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Purpose To report the presence of normal limbal epithelium detected by in vivo confocal laser scanning microscopy (IVCM) in three cases of clinically diagnosed total limbal stem cell deficiency (LSCD). Methods This is a retrospective case report consists of three patients who were diagnosed with total LSCD based on clinical exam and/or impression cytology. Clinical data including ocular history, presentation, slit-lamp examination, IVCM and impression cytology were reviewed. Results The etiology was chemical burn in three cases. One patient has two failed penetrating keratoplasty. Another had allogeneic keratolimbal transplantation but the graft failed one year after surgery. The third patient had failed amniotic membrane transplantation. These three patients presented with signs of total LSCD including the absence of normal Vogt palisades, complete superficial vascularization of the peripheral cornea, non-healing epithelial defects, and corneal scarring. Impression cytology was performed in two cases to confirm the presence of goblet cells in two cases. Each patient however still had distinct areas of corneal and/or limbal epithelial cells detected by IVCM. Conclusions Residual normal limbal epithelial cells could be present in eyes with clinical features of total LSCD. IVCM appears to be a more accurate method to evaluate the degree of LSCD.

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“…Optical PK following the COMET treatment has been reported by a group to achieve good improvement in the visual outcome with a stable corneal graft (Ma et al 2009 ). Recent studies have also shown that mesenchymal stem cells derived from various sources such as bone marrow, dental pulp, adipose tissues and limbal stroma are effective in controlling infl ammation and regeneration of corneal surface (Gu et al 2009 ;Gomes et al 2010 ;Nieto-Miguel et al 2013 ;Rohaina et al 2014 ;Katikireddy et al 2014 ;Acar et al 2015 ;Syed-Picard et al 2015 ). Another group has reported the presence of stromal stem cells at the limbal niche and demonstrated the usefulness of these autologous, mesenchymal-like stem cells in the treatment of corneal stromal scars (Li et al 2012 ;Basu et al 2014 ).…”

Section: Alternate Stem Cell Sources and Treatment Modalities For Ocumentioning

confidence: 99%

Regenerative Therapies for the Ocular Surface

Vemuganti¹,

Sangwan²,

Mariappan³

et al. 2016

Regenerative Medicine - From Protocol to Patient

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Integrity of ocular surface depends on adequate tear fi lm and stability of the surface epithelium consisting of two specialized phenotypically different epithelial cells, the central transparent corneal epithelium and the peripheral conjunctival cells, separated by a more specialized transition zone, called the limbus. Similar to the epithelial regeneration in other parts of the body, the corneal epithelium is regenerated from the stem cells located in limbus. Severe chemical burns and other diseases can cause damage to the limbus, resulting in a condition called Limbal Stem Cell Defi ciency (LSCD). Effective therapeutic modalities for this visionthreatening condition include use of human amniotic membrane, replenishing the stores of limbal stem cells by limbal transplantation. However last decade has witnessed the use of ex-vivo expanded sheet of limbal epithelial cells for ocular surface reconstruction in such cases. Our group has established a simple, feeder-cell free, cost-effective way of culturing the corneal epithelium from limbal tissues within 2 weeks, using human amniotic membrane as a vehicle. The interim results of a clinical trial involving 700 patients with severe unilateral and bilateral LSCD revealed

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Reconstruction of limbal stem cell deficient corneal surface with induced human bone marrow mesenchymal stem cells on amniotic membrane

Cited by 81 publications

References 31 publications

Concise Review: The Coming of Age of Stem Cell Treatment for Corneal Surface Damage

Concise Review: The Coming of Age of Stem Cell Treatment for Corneal Surface Damage

Existence of Normal Limbal Epithelium in Eyes With Clinical Signs of Total Limbal Stem Cell Deficiency

Regenerative Therapies for the Ocular Surface

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