Reconstruction of isolated mandibular bone defects with non-vascularized corticocancellous bone autograft and graft viability

Akbay, Ercan; Aydogan, Fusun

doi:10.1016/j.anl.2013.07.002

Cited by 27 publications

(40 citation statements)

References 12 publications

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“…Conventional allografts and autografts have limitations such as immune rejection, disease transmission, malunion, and flap necrosis [1, 2]. Researchers are developing new alternatives to traditional methods for bone defect regeneration [3]. The development of artificial bone transplantation has been greatly facilitated by tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded nHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

Zhang

Liu

et al. 2019

BioMed Research International

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Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to develop and characterize a nano-hydroxyapatite (nHA)/collagen I (ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold loaded with recombinant bone morphogenetic protein-9 (BMP-9) for bone tissue engineering by in vitro and in vivo experiments. The composite nHA/ColI/MWCNT scaffolds were fabricated at various concentrations of MWCNTs (0.5, 1, and 1.5% wt) by blending and freeze drying. The porosity, swelling rate, water absorption rate, mechanical properties, and biocompatibility of scaffolds were measured. After loading with BMP-9, bone marrow mesenchymal stem cells (BMMSCs) were seeded to evaluate their characteristics in vitro and in a critical sized defect in Sprague-Dawley rats in vivo. It was shown that the 1% MWCNT group was the most suitable for bone tissue engineering. Our results demonstrated that scaffolds loaded with BMP-9 promoted differentiation of BMMSCs into osteoblasts in vitro and induced more bone formation in vivo. To conclude, nHA/ColI/MWCNT scaffolds loaded with BMP-9 possess high biocompatibility and osteogenesis and are a good candidate for use in bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded nHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

Zhang

Liu

et al. 2019

BioMed Research International

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“…While each has distinct advantages, these options are not without their shortcomings. Autografts, generally procured from the cranium, iliac crest, ribs, tibia and other areas are considered to be the gold standard in clinical care due to their osteoconductive and osteoinductive nature [ 1 – 2 ]. However, while this approach epitomizes the clinical dogma of replacing “like with like,” defect size can preclude this option due to the relative limited supply of available autologous tissue.…”

Section: Introductionmentioning

confidence: 99%

Repair of critical sized cranial defects with BMP9-transduced calvarial cells delivered in a thermoresponsive scaffold

Dumanian

Tollemar

et al. 2017

PLoS ONE

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Large skeletal defects caused by trauma, congenital malformations, and post-oncologic resections of the calvarium present major challenges to the reconstructive surgeon. We previously identified BMP-9 as the most osteogenic BMP in vitro and in vivo. Here we sought to investigate the bone regenerative capacity of murine-derived calvarial mesenchymal progenitor cells (iCALs) transduced by BMP-9 in the context of healing critical-sized calvarial defects. To accomplish this, the transduced cells were delivered to the defect site within a thermoresponsive biodegradable scaffold consisting of poly(polyethylene glycol citrate-co-N-isopropylacrylamide mixed with gelatin (PPCN-g). A total of three treatment arms were evaluated: PPCN-g alone, PPCN-g seeded with iCALs expressing GFP, and PPCN-g seeded with iCALs expressing BMP-9. Defects treated only with PPCN-g scaffold did not statistically change in size when evaluated at eight weeks postoperatively (p = 0.72). Conversely, both animal groups treated with iCALs showed significant reductions in defect size after 12 weeks of follow-up (BMP9-treated: p = 0.0025; GFP-treated: p = 0.0042). However, H&E and trichrome staining revealed more complete osseointegration and mature bone formation only in the BMP9-treated group. These results suggest that BMP9-transduced iCALs seeded in a PPCN-g thermoresponsive scaffold is capable of inducing bone formation in vivo and is an effective means of creating tissue engineered bone for critical sized defects.

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“… 10 For a small range of bone defects (<4 cm), the clinical use of bone graft surgery for repair of bone defects may have a relatively good prognosis. For large bone defects (>4 cm), the most commonly used techniques at home and abroad include: 1) vascularized autologous bone grafts, 11 , 12 such as the use of vascularized free fibula grafts to repair large segments of bone defects from various causes, but the shortcomings include a high demand for the level of microsurgical techniques, inaccurate effects of vascular anastomosis, long time of creeping substitution for bone graft, and repairable bone defects limited by the length and diameter of the donor area, as well as multiple complications in the recipient area, making it not an ideal procedure for infected bone defects. 2) The technique of distraction osteogenesis 13 - 15 i.e.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-loaded bone cement spacer usage combined with membrane induction in infected gap non-unions: A case series

Li¹,

Ding²,

Zhou³

et al. 2018

Pak J Med Sci

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Objective:To explore the clinical effects upon gap nonunion of antibiotic-loaded bone cement spacer combined with membrane induction on infected bone defects.Methods:The data of 16 patients with infected bone defects admitted in General Hospital of Shenyang Military Area Command from January 2009 to January 2011 were analyzed retrospectively. There were 12 males and 4 females aged between 24-63 years age (average 43.1 ± 9.7) who had received antibiotic laiden bone cement spacer treatment. Stage-1, debridement and anti-biotic treatment with intraoperative preparation of customized bone cement spacers (antibiotics and bone cement spacer) with or without internal or external fixation Stage-2, removal of spacer and repair of bone defects using membrane-induced technique and internal fixation at bone defects site.Results:Sixteen patients were followed up for 39-98 months, (67.2 ± 20.4) on average. All patients with infected bone defects were healed. X-ray showed that fractures had healed and the new bone formed at graft site was more radio opaque than that of adjacent bone segments. The healing time was 6 to 10 months, (7.4 ± 1.1) on average. There was no recurrence of infection or deformity.Conclusion:The antibiotic-loaded cement spacer can control the local infection while maintaining the limb length and increasing the stability, reducing the contracture of bone and soft tissue, creating conditions for subsequent repair and reducing the infection rate of bone defects.

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Reconstruction of isolated mandibular bone defects with non-vascularized corticocancellous bone autograft and graft viability

Cited by 27 publications

References 12 publications

Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded nHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded nHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

Repair of critical sized cranial defects with BMP9-transduced calvarial cells delivered in a thermoresponsive scaffold

Antibiotic-loaded bone cement spacer usage combined with membrane induction in infected gap non-unions: A case series

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