Reconstruction of intraoral oncologic surgical defects with Integra<sup>®</sup> bilayer wound matrix

Srivastava, Akanksha; Maniakas, Anastasios; Myers, Jeffrey N.; Chambers, Mark S.; Cardoso, Richard C.

doi:10.1002/ccr3.3501

Cited by 17 publications

(15 citation statements)

References 25 publications

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“…Initially, conditions were established to grow biofilms in commercially available collagen-GAG based scaffolds, that resemble the skin extracellular matrix in terms of structure and composition, which has been broadly described in research and for its clinical use for dermal regeneration 39 . Using an MTT assay, where formazan crystals remain intracellularly 40 , viable bacteria within the biomaterial were localized and quantified 40 .…”

Section: Discussionmentioning

confidence: 99%

Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds

Cárdenas-Calderón

Veloso-Giménez

González

et al. 2022

Sci Rep

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Chronic wounds cannot heal due to impairment of regeneration, mainly caused by the persistent infection of multispecies biofilms. Still, the effects of biofilm wound infection and its interaction with the host are not fully described. We aimed to study functional biofilms in physiological conditions in vitro, and their potential effects in health and regeneration in vivo. Therefore, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis were seeded in collagen-based scaffolds for dermal regeneration. After 24 h, scaffolds had bacterial loads depending on the initial inoculum, containing viable biofilms with antibiotic tolerance. Afterwards, scaffolds were implanted onto full skin wounds in mice, together with daily supervision and antibiotic treatment. Although all mice survived their health was affected, displaying fever and weight loss. After ten days, histomorphology of scaffolds showed high heterogeneity in samples and within groups. Wounds were strongly, mildly, or not infected according to colony forming units, and P. aeruginosa had higher identification frequency. Biofilm infection induced leucocyte infiltration and elevated interferon-γ and interleukin-10 in scaffolds, increase of size and weight of spleen and high systemic pro-calcitonin concentrations. This functional and implantable 3D biofilm model allows to study host response during infection, providing a useful tool for infected wounds therapy development.

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Section: Discussionmentioning

confidence: 99%

Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds

Cárdenas-Calderón

Veloso-Giménez

González

et al. 2022

Sci Rep

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“…Case reports have reported the feasibility of substitutes including acellular dermal homograft, bilayer wound matrix, and porcine submucosal grafts. However, there remains limited evidence on the objective benefits of these reconstructive alternatives [5 ▪ ,6,7].…”

Section: Biologics and Skin Graftsmentioning

confidence: 99%

Advances in oral tongue reconstruction: a reconstructive paradigm and review of functional outcomes

Thompson

Vakharia

Hatten

2022

Current Opinion in Otolaryngology &Amp; Head &Amp; Neck Surgery

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Purpose of reviewGlossectomy remains a common treatment of oral tongue malignancies, which has a range of functional impacts depending on the extent of resection. This review aims to categorize and provide context for the approach to reconstructing these defects using recent evidence. Recent findingsThe reconstruction method of choice should be tailored to the size and location of the glossectomy defect with special consideration to replace tongue volume and preserve mobility. There has been an increasing focus on patient-reported outcomes in oral tongue reconstruction. For defects beyond one-third of the tongue, free tissue reconstruction, and more recently, the submental artery island flap yield excellent results. Advances in reconstruction of larger defects have included preoperative soft tissue planning and assessment of outcomes in total glossectomy patients with laryngeal preservation.

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“…Often the post-ablative defect is not small enough to be closed for first intention, given the small amount of usable tissue in the mouth. Moreover, second-intention healing in the oral cavity could result in scarring and consequent contractures with functional defects [ 1 ]. Patients undergoing demolitive surgery for oral cancer treatment and free flap reconstruction might require surgery in the years following the first surgical treatment to obtain a neo-fornix and reduce scar retraction [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…At the moment, for intraoral defects not requiring regional or free flap for closure, it is possible to use autologous full-thickness or split-thickness grafts. The harvesting procedure of those grafts is not free of risks, including donor site infections, functional or aesthetic deficits, and patient discomfort [ 1 ]. The use of biosynthetic skin substitutes is increasing to avoid the risks above.…”

Section: Introductionmentioning

confidence: 99%

Dermal Regeneration Template: Reconstruction in Oral Cancer Defects

Mangini

Galvano

Pucci

et al. 2023

J. Maxillofac. Oral Surg.

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Background Post ablative oral mucosal defect resulting from the removal of tumors can be treated with various techniques. Purpose In this paper, we are showing what, in our experience, are the advantages and disadvantages given using biosynthetic skin substitutes when dealing with this kind of lesions. Materials and methods Patients included in the sample came to our attention with both neoplastic lesions (11 subjects) and important scar retraction after previous oncologic surgery (1 subject). All patients underwent trans-oral resection surgery following the same surgical protocol and post ablative oral mucosal defect were treated using the dermal regeneration template. The surgical defect location, size, and time of removal of the silicone layer varied from one subject to the other. Results Most patients showed good healing with reduced scarring and adequate remucosalisation of the defect. The main complications were shown in a palatal lesion treated with concomitant osteal resection, which developed an oroantral fistula at follow up, and tongue lesions which showed some scarring. Conclusions Given our experience, we would advise using dermal substitutes when reconstructing oral defects only after a cautious evaluation of the area of the lesion, the gap size, the possible adherence of the membrane to the gap, and the presence of tissue supporting the overlying membrane.

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Reconstruction of intraoral oncologic surgical defects with Integra^® bilayer wound matrix

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 17 publications

References 25 publications

Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds

Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds

Advances in oral tongue reconstruction: a reconstructive paradigm and review of functional outcomes

Dermal Regeneration Template: Reconstruction in Oral Cancer Defects

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Reconstruction of intraoral oncologic surgical defects with Integra® bilayer wound matrix

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 17 publications

References 25 publications

Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds

Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds

Advances in oral tongue reconstruction: a reconstructive paradigm and review of functional outcomes

Dermal Regeneration Template: Reconstruction in Oral Cancer Defects

Contact Info

Product

Resources

About

Reconstruction of intraoral oncologic surgical defects with Integra^® bilayer wound matrix