Reconstruction of functional tissues with cell sheet engineering

Yang, Joseph; Yamato, Masayuki; Shimizu, Tatsuya; Sekine, Hidekazu; Ohashi, Kazuhiko; Kanzaki, Masato; Ohki, Takeshi; Nishida, Kohji; Okano, Teruo

doi:10.1016/j.biomaterials.2007.07.052

Cited by 443 publications

(323 citation statements)

References 57 publications

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“…Thermosensitive chitosan has also been investigated to create an aligned cell sheet [32]. Cell sheet engineering approach is excellent for epithelium, endothelium and cell-dense tissues [111,123] as the formation of cell sheets require cells to grow into confluence at high density such that cells can form tight junctions with each other and secrete ECM of their own. High cell density and close association is a characteristic of epithelium and endothelium.…”

Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

“…The other disadvantage or perhaps limitation of cell sheet engineering approach is that constructing ECM rich tissues and hypocellular tissue such as bones, cartilage and intervertebral disc are unlikely as the amount of ECM secreted upon cell confluence is severely limited. In another word, tissues with rich ECM for load bearing purposes are unlikely to be fabricated by a cell sheet engineering approach [123].…”

Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

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Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

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Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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“…A confluent cultured cell monolayer, which is detached from the PIPAAm-grafted cell culture substrate by decreasing the temperature, is used in regenerative medicine [24][25][26]. Thermoresponsive cell culture dishes, grafted by controlling the thickness of the thermoresponsive polymeric layer, allow recovery of confluent cell [27,28].…”

Section: Temp Increase Immobilized Pipaam Moleculesmentioning

confidence: 99%

“…A new approach called 'cell sheet engineering' has been used to construct ideal transplantable tissues composed exclusively of cells. Our group has developed a novel means of constructing three-dimensional tissues from cell sheets using a thermoresponsive cell culture dish [24][25][26]. The recovered cell sheets bear the ECM on the bottom side, and, as a result, it is possible to transfer the cell sheets onto other materials, such as a culture dish, another cell sheet, or a biological tissue ( figure 2(b)).…”

Section: Cell Sheet-based Tissue Engineering and Its Clinical Applicamentioning

confidence: 99%

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Kobayashi¹,

Okano²

2010

Science and Technology of Advanced Materials

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This article reviews the properties and characterization of an intelligent thermoresponsive surface, which is a key technology for cell sheet-based tissue engineering. Intelligent thermoresponsive surfaces grafted with poly(N-isopropylacrylamide) exhibit hydrophilic/hydrophobic alteration in response to temperature change. Cultured cells are harvested on thermoresponsive cell culture dishes by decreasing the temperature without the use of digestive enzymes or chelating agents. Our group has developed cell sheet-based tissue engineering for therapeutic uses with single layer or multilayered cell sheets, which were recovered from the thermoresponsive cell culture dish. Using surface derivation techniques, we developed a new generation of thermoresponsive cell culture dishes to improve culture conditions. We also designed a new methodology for constructing well-defined organs using microfabrication techniques.

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“…6 Other studies using epidermal keratinocytes have shown that it was possible to maintain the differentiated functions after the cell sheets were collected. 7 However, although thermoresponsive culture surfaces have shown success in producing whole sheets of hard, cell-dense tissues such as myocardium, the technique is not suitable for tissue patterning and for soft tissue. For example, biological soft tissue like brain and adipose are difficult to manipulate (for the mechanical shock that is applied briefly to the hydrogel substrate to dislodge the sheets, may cause soft tissue to collapse rather than dislodge or detach).…”

Section: Introductionmentioning

confidence: 99%

Cell sheet patterning using photo-cleavable polymers

Apostol

Mironava

Yang

et al. 2011

Polym J

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We have found that poly (4-vinylpyridine) (P 4 VP) and poly (methylmethacrylate) thin films can be etched with ultraviolet A (UVA) radiation. Furthermore, we also found that dermal fibroblasts could be cultured successfully on the P 4 VP polymer, with a doubling time comparable to tissue culture Petri dish standards. Consequently, we were able to grow tissue on P 4 VP substrates, this could easily be lifted using UVA radiation. The cell sheets that were removed were then re-plated at a lower density and a series of assays was performed at 3 and 6 days. Although only a small amount of damage was discernable at day 3 nearly complete recovery was observed at day 6. The technique was used to pattern areas within the tissue, where other types of cells could be inserted. To demonstrate the technique, a hybrid tissue layer was produced, in which the dermal fibroblasts in a circular area at the center of the sample were removed by exposure through a mask. A keratinocyte layer was inserted, which adhere to the fibroblast layer forming a tissue with integrated layers of two distinct cell types. Polymer Journal (2011) 43, 723-732; doi:10.1038/pj.2011; published online 8 June 2011Keywords: biopolymers; cells INTRODUCTION A significant shortcoming of conventional tissue engineering methods is the loss of differentiated cell functions during cell collecting. Typical cell collecting techniques use proteolytic enzymes such as trypsin or dispase, which irreversibly damage cell surface proteins, such as growth factor receptors, ion channels and cell-to-cell junction proteins. 1 This result in an inability to assemble constructs from multiple cell types, which is an important requirement in tissue engineering.To address these shortcomings, cell sheet engineering has been proposed as a new methodology to facilitate the non-invasive collect of cultured cells as intact cell sheets. 2 As the cell sheets collected through this technique are recovered with intact cell-to-cell junctions and deposited extracellular matrix whole-cell sheets can be transferred to other tissue surfaces to produce versatile two-dimensional or threedimensional tissues. 3 Cell sheet engineering advanced rapidly with the development of culture dishes covalently grafted with temperatureresponsive polymers. These culture surfaces allowed whole, contiguous cell sheets to spontaneously detach from the surface simply by briefly decreasing the temperature. 4,5 This technique was also used to culture four layers of cardiomyocytes, which successfully formed pulsatile myocardial tissue in vitro and in vivo. 6 Other studies using epidermal keratinocytes have shown that it was possible to maintain the differentiated functions after the cell sheets were collected. 7 However, although thermoresponsive culture surfaces have shown success in producing whole sheets of hard, cell-dense tissues such as myocardium, the technique is not suitable for tissue patterning and for soft tissue. For example, biological soft tissue like brain and adipose are difficult to manipulate (for the...

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Reconstruction of functional tissues with cell sheet engineering

Cited by 443 publications

References 57 publications

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Cell sheet patterning using photo-cleavable polymers

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