Reconstruction and optimization of a Pseudomonas putida-Escherichia coli microbial consortium for mcl-PHA production from lignocellulosic biomass

Qin, Ruolin; Zhu, Yinzhuang; Ai, Mingmei; Jia, Xiaoqiang

doi:10.3389/fbioe.2022.1023325

Cited by 10 publications

(3 citation statements)

References 72 publications

(86 reference statements)

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“…A bacterial consortium was created in which xylose is first converted to acetate by E. coli before being utilized for PHA production by Pseudomonas putida. This was achieved through the deletion of glucose uptake, suppression of respiration, promotion of fatty acid export, deletion of PHA depolymerase, and beta-oxidation pathway (1.30 g/L, %PHA not determined) [69]. To ensure safety, aerobic cultivation on H 2 requires a low concentration of O 2 .…”

Section: Overview Of Artificial Modifications To Enhance Pha Productionmentioning

confidence: 99%

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Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Fukala,

Kučera

2024

Molecules

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Polyhydroxyalkanoates (PHAs) are intracellular biopolymers that microorganisms use for energy and carbon storage. They are mechanically similar to petrochemical plastics when chemically extracted, but are completely biodegradable. While they have potential as a replacement for petrochemical plastics, their high production cost using traditional carbon sources remains a significant challenge. One potential solution is to modify heterotrophic PHA-producing strains to utilize alternative carbon sources. An alternative approach is to utilize methylotrophic or autotrophic strains. This article provides an overview of bacterial strains employed for PHA production, with a particular focus on those exhibiting the highest PHA content in dry cell mass. The strains are organized according to their carbon source utilization, encompassing autotrophy (utilizing CO2, CO) and methylotrophy (utilizing reduced single-carbon substrates) to heterotrophy (utilizing more traditional and alternative substrates).

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Section: Overview Of Artificial Modifications To Enhance Pha Productionmentioning

confidence: 99%

“…It is also worth mentioning a bacterial consortium that utilizes xylose and glucose to produce 1.30 g of PHA per liter. However, the %PHA was not determined [69]. Glycerol is produced in large quantities as a by-product of the transesterification of natural triacylglycerides.…”

Section: Food and Other Industry Wastementioning

confidence: 99%

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Fukala,

Kučera

2024

Molecules

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“…A coculture consortium comprising engineered Pseudomonas putida and Escherichia coli was functionally assembled for MCL-PHA production from a glucose-xylose mixture (Liu et al, 2020). Furthermore, the reconstructed P. putida-E. coli consortium was capable of producing 1.30 and 1.02 g/L MCL-PHA from a glucose-xylose mixture and lignocellulosic hydrolysate, respectively (Qin et al, 2022;Zhu, Ai, et al, 2022). In addition, a Corynebacterium glutamicum cell factory was constructed for the production of poly(3-hydroxybutyrate) (PHB) from lignocellulose via systematic metabolic engineering (Jin et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9

Wang,

Liu,

Guo

et al. 2024

Biotech & Bioengineering

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Microbial production of polyhydroxyalkanoate (PHA) is greatly restricted by high production cost arising from high‐temperature sterilization and expensive carbon sources. In this study, a low‐cost PHA production platform was established from Halomonas cupida J9. First, a marker‐less genome‐editing system was developed in H. cupida J9. Subsequently, H. cupida J9 was engineered to efficiently utilize xylose for PHA biosynthesis by introducing a new xylose metabolism module and blocking xylonate production. The engineered strain J9UΔxylD‐P8xylA has the highest PHA yield (2.81 g/L) obtained by Halomonas with xylose as the sole carbon source so far. This is the first report on the production of short‐ and medium‐chain‐length (SCL‐co‐MCL) PHA from xylose by Halomonas. Interestingly, J9UΔxylD‐P8xylA was capable of efficiently utilizing glucose and xylose as co‐carbon sources for PHA production. Furthermore, fed‐batch fermentation of J9UΔxylD‐P8xylA coupled to a glucose/xylose co‐feeding strategy reached up to 12.57 g/L PHA in a 5‐L bioreactor under open and unsterile condition. Utilization of corn straw hydrolysate as the carbon source by J9UΔxylD‐P8xylA reached 7.0 g/L cell dry weight (CDW) and 2.45 g/L PHA in an open fermentation. In summary, unsterile production in combination with inexpensive feedstock highlights the potential of the engineered strain for the low‐cost production of PHA from lignocellulose‐rich agriculture waste.

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Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Baltacı,

Görmez

et al. 2024

Environ Sci Pollut Res

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Polyhydroxyalkanoates have attracted great interest as a suitable alternative to petrochemical based plastics due to their outstanding properties such as biodegradability and biocompatibility. However, the biggest problem in the production of microbial polyhydroxyalkanoates is low cost-effectiveness. In this study, polyhydroxyalkanoate production was carried out using waste substrates with local isolates. Culture conditions were optimized to increase the polyhydroxyalkanoate production potential. The produced polyhydroxyalkanoate was characterized by FTIR analyses, and its metabolic pathway was determined by real-time PCR. According to the results, the best polyhydroxyalkanoate producer bacteria was characterized as Pseudomonas neustonica NGB15. The optimal culture conditions were detected as 30 g/L banana peel powder, 25 °C temperature, pH 8, and 4-day incubation time. Under the optimized conditions, 3.34 g/L PHA production was achieved. As a result of FTIR analyses, major peaks were obtained at 1723, 1277, 1261, 1097, 1054, and 993 cm−1. These peaks represent that the type of produced polyhydroxyalkanoate was poly-β-hydroxybutyrate. According to gene expression profile of NGB15, it was determined that Pseudomonas neustonica NGB15 produces PHA using the de novo fatty acid synthesis metabolic pathway. In conclusion, poly-β-hydroxybutyrate production by Pseudomonas neustonica NGB15 using a low-cost fermentation medium has been shown to be biotechnologically promising.

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Reconstruction and optimization of a Pseudomonas putida-Escherichia coli microbial consortium for mcl-PHA production from lignocellulosic biomass

Cited by 10 publications

References 72 publications

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

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