Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs

Abukawa, Harutsugi; Zhang, Weibo; Young, Conan S.; Asrican, Rose; Vacanti, Joseph P.; Kaban, Leonard B.; Troulis, Maria J.; Yelick, Pamela C.

doi:10.1016/j.joms.2008.09.002

Cited by 75 publications

(43 citation statements)

References 32 publications

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“…Some studies have demonstrated that miniature pigs serve as an excellent translational model for autologous stem cells in transplantation (Sonoyama et al, 2006;Oltramari et al, 2007). In addition, miniature pigs are frequently used for tooth and periodontal regeneration research (Ibi et al, 2007;Liu et al, 2008;Abukawa et al, 2009;Ando et al, 2009;Honda et al, 2009;Zhang et al, 2009;Zheng et al, 2009;Ding et al, 2010). However, despite the fact that miniature pigs are useful laboratory animals, there is little information on miniature pig teeth.…”

Section: Discussionmentioning

confidence: 99%

Postnatal Mandibular Cheek Tooth Development in the Miniature Pig Based on Two‐Dimensional and Three‐Dimensional X‐Ray Analyses

Ide

Nakahara

Nasu

et al. 2013

The Anatomical Record

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The miniature pig is a useful large laboratory animal model. Various tissues and organs of miniature pigs are similar to those of humans in terms of developmental, anatomical, immunological, and physiological characteristics. The oral and maxillofacial region of miniature pigs is often used in preclinical studies of regenerative dentistry. However, there is limited information on the dentition and tooth structure of miniature pigs. The purpose of this study was to examine the time-course changes of dentition and tooth structure (especially the root) of the miniature pig mandibular cheek teeth through X-ray analyses using soft X-ray for twodimensional observations and micro-CT for three-dimensional observations. The mandibles of male Clawn strain miniature pigs (2 weeks and 3, 5, 7, 9, 11, 14, 17, and 29 months of age) were used. X-ray analysis of the dentition of miniature pig cheek teeth showed that the eruption pattern of the miniature pig is diphyodont and that the replacement pattern is vertical. Previous definitions of deciduous and permanent teeth often varied and there has been no consensus on the number of teeth (dentition); however, we found that three molars are present in the deciduous dentition and that four premolars and three molars are present in the permanent dentition. Furthermore, we confirmed the number of tooth roots and root canals. We believe that these findings will be highly useful in future studies using miniature pig teeth.

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Section: Discussionmentioning

confidence: 99%

Postnatal Mandibular Cheek Tooth Development in the Miniature Pig Based on Two‐Dimensional and Three‐Dimensional X‐Ray Analyses

Ide

Nakahara

Nasu

et al. 2013

The Anatomical Record

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“…E14.5 oral epithelium and dental mesenchyme were reconstituted in collagen gels and cultured ex vivo [27], and when implanted into the maxillary molar extraction sockets in 5-week-old mice, tooth morphogenesis took place and was followed by eruption into occlusion [28]. Several studies have begun to tackle an obligatory task of scale up towards human tooth size [38][39]. Thus, tooth regeneration by cell transplantation is a meritorious approach.…”

Section: Embryonic Endothelium: E10 Membrane Filtersmentioning

confidence: 99%

Tooth regeneration: a revolution in stomatology and evolution in regenerative medicine

Yıldırım

Kim

et al. 2011

Int J Oral Sci

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A tooth is a complex biological organ and consists of multiple tissues including the enamel, dentin, cementum and pulp. Tooth loss is the most common organ failure. Can a tooth be regenerated? Can adult stem cells be orchestrated to regenerate tooth structures such as the enamel, dentin, cementum and dental pulp, or even an entire tooth? If not, what are the therapeutically viable sources of stem cells for tooth regeneration? Do stem cells necessarily need to be taken out of the body, and manipulated ex vivo before they are transplanted for tooth regeneration? How can regenerated teeth be economically competitive with dental implants? Would it be possible to make regenerated teeth affordable by a large segment of the population worldwide? This review article explores existing and visionary approaches that address some of the above-mentioned questions. Tooth regeneration represents a revolution in stomatology as a shift in the paradigm from repair to regeneration: repair is by metal or artificial materials whereas regeneration is by biological restoration. Tooth regeneration is an extension of the concepts in the broad field of regenerative medicine to restore a tissue defect to its original form and function by biological substitutes.

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“…Recent advancements in bone-engineering techniques have made it possible to provide alternatives for new regenerative treatment, including cellbased bone regeneration by autologous cell transplantation (Noshi et al, 2001;Abukawa et al, 2009;Park et al, 2011), because this technique requires only a small amount of tissue from the patients and can be used to repair bone defects without leading to donor site morbidity.…”

Section: Introductionmentioning

confidence: 99%

Bone Regeneration at Dental Implant Sites with Suspended Stem Cells

Zheng

Park

Cho³

et al. 2014

J Dent Res

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During the maintenance of bone marrow-derived mesenchymal stem cells (BMMSCs), suspended cells are discarded normally. We noted the osteogenic potential of these cells to be like that of anchorage-dependent BMMSCs. Therefore, we characterized suspended BMMSCs from rabbit bone marrow by bioengineering and applied the suspended BMMSCs to double-canaled dental implants inserted into rabbits. After primary isolation of BMMSCs, we collected the suspended cells during primary culture on the third day. The cells were transferred and maintained on an extracellular-matrix-coated culture plate. The cells were characterized and compared with BMMSCs by colony-forming-unit fibroblast (CFU-f) and cell proliferation assay, fluorescence-activated cell sorter (FACS), in vitro multipotency, and reverse transcription polymerase chain reaction (RT-PCR). We also analyzed the osteogenic potential of cells mixed with hydroxyapatite/tricalcium phosphate (HA/TCP) and transplanted into immunocompromised mice. We compared the viability and proliferation of the suspended BMMSCs and BMMSCs on the titanium implant surface and observed cell morphology. Then, the cells mixed with HA/TCP were applied to the double-canaled implants during installation into rabbit tibia. Four weeks later, we analyzed bone formation inside the canal by histomorphometry. The suspended cells showed higher CFU-f on the extracellular matrix (ECM)-coated culture plate and similar results of proliferation capacity compared with BMMSCs. The cells also showed osteogenic, adipogenic, and chondrogenic ability. The suspended cells showed levels of attachment survival and proliferation on the surfaces of titanium implant discs to be higher than or similar to those of BMMSCs. The suspended cells as well as BMMSCs showed stronger bone formation ability in both upper and lower canals of the implants compared with controls on double-canaled implants inserted into rabbit tibia. In this study, we showed that suspended cells after primary BMMSC isolation have bone regeneration capacity like that of BMMSCs, not only in vitro but also in vivo. ECM was valuable for propagation of MSCs for cell-based bone regeneration. Therefore, the suspended cells could also be useful tools for bone regeneration after implant surgery.

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Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs

Cited by 75 publications

References 32 publications

Postnatal Mandibular Cheek Tooth Development in the Miniature Pig Based on Two‐Dimensional and Three‐Dimensional X‐Ray Analyses

Postnatal Mandibular Cheek Tooth Development in the Miniature Pig Based on Two‐Dimensional and Three‐Dimensional X‐Ray Analyses

Tooth regeneration: a revolution in stomatology and evolution in regenerative medicine

Bone Regeneration at Dental Implant Sites with Suspended Stem Cells

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