Reconstructing human skin equivalents on fibrin-based dermal matrix

Sriram, Gopu; Dykas, Michal Marcin; Ramasamy, Srinivas; Poddar, Kingshuk; Krishnan-Kutty, Viknish; Patra, Abhijeet; Venkatesan, T.; Bigliardi-Qi, Mei; Bigliardi, Paul L.

doi:10.1016/j.mattod.2016.01.015

Cited by 5 publications

(1 citation statement)

References 21 publications

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“…The results were compared with HSEs derived from primary cells and ex vivo skin cultures. The results were promising, although additional models such as fibrin‐based skin equivalents should be investigated before establishing immortalised keratinocytes as a standard procedure. Accordingly, the protocol established by Carlson and colleagues can be considered as the current standard as this includes considerations of the microenvironment and allows growth of tissue in a variety of extracellular matrix substrates, amongst other advantages.…”

Section: Related Workmentioning

confidence: 99%

Optical investigation of three‐dimensional human skin equivalents: A pilot study

Kallepalli

McCall

James

et al. 2019

Journal of Biophotonics

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Human skin equivalents (HSEs) are three‐dimensional living models of human skin that are prepared in vitro by seeding cells onto an appropriate scaffold. They recreate the structure and biological behaviour of real skin, allowing the investigation of processes such as keratinocyte differentiation and interactions between the dermal and epidermal layers. However, for wider applications, their optical and mechanical properties should also replicate those of real skin. We therefore conducted a pilot study to investigate the optical properties of HSEs. We compared Monte Carlo simulations of (a) real human skin and (b) two‐layer optical models of HSEs with (c) experimental measurements of transmittance through HSE samples. The skin layers were described using a hybrid collection of optical attenuation coefficients. A linear relationship was observed between the simulations and experiments. For samples thinner than 0.5 mm, an exponential increase in detected power was observed due to fewer instances of absorption and scattering.

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Section: Related Workmentioning

confidence: 99%

Optical investigation of three‐dimensional human skin equivalents: A pilot study

Kallepalli

McCall

James

et al. 2019

Journal of Biophotonics

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Nanofibered Gelatin‐Based Nonwoven Elasticity Promotes Epithelial Histogenesis

Jedrusik

Meyen

Finkenzeller

et al. 2018

Adv Healthcare Materials

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Regarding tissue regeneration, mechanics of biomaterials gains progressive importance. Therefore, this study reports on in situ crosslinked electrospun gelatin nonwoven mats (NWMs) whose distinct modulus of elasticity (ME) promotes epithelial tissue formation in a graded manner. NWMs, comprising fiber diameters in various distributions, yield an ME of about 2.1, 3.2, and 10.9 kPa. A two-step approach of preclinical in vitro validation identifies the elasticity of 3.2 kPa as superior to the other, regarding the histogenetic epithelial outcome. Hence, this 3.2 kPa candidate NWM is colonized with oral mucosal epithelial keratinocytes in the absence or presence of mesenchymal fibroblasts and/or endothelial cells. Evaluation of epithelial histogenesis at days 1 to 10 occurs by colorimetric and fluorescence-based immunohistochemistry (IHCH) of specific biomarkers. These include cytokeratins (CK) 14, CK1, and involucrin that indicate different stages of epithelial differentiation, as well as the basement membrane constituent collagen type IV and Ki-67 as a proliferation marker. Intriguingly, histogenesis and IHCH reveal the best resemblance of the native epithelium by the NWM alone, irrespective of other cell counterparts. These findings prove the gelatin NWM a convenient cell matrix, and evidence that NWM mechanics is important to promote epithelial histogenesis in view of prospective clinical applications.

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