Reconstructing historical trends of polycyclic aromatic hydrocarbon deposition in a remote area of Spain using herbarium moss material

Foan, Louise; Sablayrolles, Caroline; Elustondo, David; Lasheras, E.; González, Lucy T.; Ederra, A.; Simon, Valérie; Santamaría, Jesús Miguel

doi:10.1016/j.atmosenv.2010.05.019

Cited by 47 publications

(48 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polycyclic aromatic hydrocarbons (PAHs) are are ubiquitous in the environment and concentrations increase with human population growth and fossil fuel use (Van Metre et al 2000; Foan et al 2010; Yang et al 2011). Aquatic environments are susceptible to PAH contamination in a variety of ways including oil shipping, oil refining, industrial outfall, wastewater discharges, urban runoff, and atmospheric deposition (Van Metre, Mahler et al 2000; Walker et al 2004; Van Metre and Mahler 2005; Hylland 2006).…”

Section: Introductionmentioning

confidence: 99%

Developmental exposure to a complex PAH mixture causes persistent behavioral effects in naive Fundulus heteroclitus (killifish) but not in a population of PAH-adapted killifish

Brown

Bailey

Oliveri

et al. 2016

Neurotoxicology and Teratology

View full text Add to dashboard Cite

Acute exposures to some individual polycyclic aromatic hydrocarbons (PAHs) and complex PAH mixtures are known to cause cardiac malformations and edema in the developing fish embryo. However, the heart is not the only organ impacted by developmental PAH exposure. The developing brain is also affected, resulting in lasting behavioral dysfunction. While acute exposures to some PAHs are teratogenically lethal in fish, little is known about the later life consequences of early life, lower dose subteratogenic PAH exposures. We sought to determine and characterize the long-term behavioral consequences of subteratogenic developmental PAH mixture exposure in both naive killifish and PAH-adapted killifish using sediment pore water derived from the Atlantic Wood Industries Superfund Site. Killifish offspring were embryonically treated with two low-level PAH mixture dilutions of Elizabeth River sediment extract (ERSE) (TPAH 5.04 μg/L and 50.4 μg/L) at 24 hours post fertilization. Following exposure, killifish were raised to larval, juvenile, and adult life stages and subjected to a series of behavioral tests including: a locomotor activity test (4 days post-hatch), a sensorimotor response tap/habituation test (3 months post hatch), and a novel tank diving and exploration test (3 months post hatch). Killifish were also monitored for survival at 1, 2, and 5 months over 5-month rearing period. Developmental PAH exposure caused short-term as well as persistent behavioral impairments in naïve killifish. In contrast, the PAH-adapted killifish did not show behavioral alterations following PAH exposure. PAH mixture exposure caused increased mortality in reference killifish over time; yet, the PAH-adapted killifish, while demonstrating long-term rearing mortality, had no significant changes in mortality associated with ERSE exposure. This study demonstrated that early embryonic exposure to PAH-contaminated sediment pore water caused long-term locomotor and behavioral alterations in killifish, and that locomotor alterations could be observed in early larval stages. Additionally, our study highlights the resistance to behavioral alterations caused by low-level PAH mixture exposure in the adapted killifish population. Furthermore, this is the first longitudinal behavioral study to use killifish, an environmentally important estuarine teleost fish, and this testing framework can be used for future contaminant assessment.

show abstract

Section: Introductionmentioning

confidence: 99%

Developmental exposure to a complex PAH mixture causes persistent behavioral effects in naive Fundulus heteroclitus (killifish) but not in a population of PAH-adapted killifish

Brown

Bailey

Oliveri

et al. 2016

Neurotoxicology and Teratology

View full text Add to dashboard Cite

show abstract

“…PAHs are ubiquitous, and their levels in the environment track human population growth and burning of fossil fuels. 4–7 Estuarine habitats are vulnerable to PAH contamination via a variety of routes, including oil shipping and refining, industrial outfalls, wastewater discharges, urban runoff, and atmospheric deposition. 8 …”

Section: Introductionmentioning

confidence: 99%

Compound- and Mixture-Specific Differences in Resistance to Polycyclic Aromatic Hydrocarbons and PCB-126 amongFundulus heteroclitusSubpopulations throughout the Elizabeth River Estuary (Virginia, USA)

Clark

Cooper²,

Stapleton³

et al. 2013

Environ. Sci. Technol.

View full text Add to dashboard Cite

Atlantic killifish (Fundulus heteroclitus) inhabiting the Atlantic Wood Industries Superfund Site (Elizabeth River, Portsmouth, VA, USA) are resistant to the acute toxicity and cardiac teratogenesis caused by high levels of polycyclic aromatic hydrocarbons (PAHs) from creosote. The resistance is linked to down regulation of the aryl hydrocarbon receptor (AHR) pathway. We investigated the association between CYP1 activity, as a marker of potential AHR pathway suppression, and contaminant resistance in killifish subpopulations from sites throughout the estuary that varied significantly in PAH contamination level. Adult killifish and sediments were collected from seven sites across approximately 13.7 kilometers in river length within the estuary and from a nearby reference site. Sediment PAH levels were determined using gas chromatography mass spectrometry. Embryos obtained via manual spawning were exposed to individual AHR agonists and PAH mixtures 24 hours post fertilization (hpf); CYP1 activity was determined by in ovo ethoxyresorufin-o-deethylase (EROD) 96 hpf and cardiac deformity severity was scored 144 hpf. The total PAH levels measured among the sites varied from approximately 200–125,000 ng/g dry sediment. Overall, the resistance to teratogenesis was strongest in the subpopulations from sites in or closest to the major PAH contamination sites, but even embryos from less-contaminated sites within the Elizabeth River demonstrated at least partial resistance to many challenges. Surprisingly, all of the subpopulations tested were highly resistant to PCB-126 (3,3′,4,4′,5-pentachlorobiphenyl). However, the degree of CYP1 activity response varied significantly among subpopulations and did not always correlate strongly with resistance to teratogenesis; some subpopulations resisted the cardiac teratogenesis caused by the challenges at doses that still elicited strong EROD induction. Our results suggest that there is variation in the adaptive phenotype exhibited by laboratory-spawned embryos from killifish subpopulations throughout the estuary. Furthermore, the results show that contaminants have affected killifish subpopulations throughout the estuary, even in sites with lower levels of PAHs.

show abstract

“…Sample treatment has traditionally involved Soxhlet [24,25] or Soxtec [26] apparatus which demand for long extraction times (3-24 h) and high volumes of organic solvents (100-200 mL of hexane, dichloromethane, acetonitrile, etc). So, recent research has focused on the simplification of moss sample treatment and techniques such as extraction assisted by ultrasounds [23,27] or microwaves [20,28] and accelerated solvent extraction [22,29] have been proposed as alternatives to the conventional ones, with extractions taking on average 30 min with 20-30 mL of M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 5 kg -1 using LC-FLD [22,31] and GC-MS [20,24,30], respectively.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured alkyl carboxylic acid-based restricted access solvents: Application to the combined microextraction and cleanup of polycyclic aromatic hydrocarbons in mosses

Caballero-Casero

Çabuk

Martínez-Sagarra

et al. 2015

Analytica Chimica Acta

View full text Add to dashboard Cite

Reconstructing historical trends of polycyclic aromatic hydrocarbon deposition in a remote area of Spain using herbarium moss material

Cited by 47 publications

References 46 publications

Developmental exposure to a complex PAH mixture causes persistent behavioral effects in naive Fundulus heteroclitus (killifish) but not in a population of PAH-adapted killifish

Developmental exposure to a complex PAH mixture causes persistent behavioral effects in naive Fundulus heteroclitus (killifish) but not in a population of PAH-adapted killifish

Compound- and Mixture-Specific Differences in Resistance to Polycyclic Aromatic Hydrocarbons and PCB-126 amongFundulus heteroclitusSubpopulations throughout the Elizabeth River Estuary (Virginia, USA)

Nanostructured alkyl carboxylic acid-based restricted access solvents: Application to the combined microextraction and cleanup of polycyclic aromatic hydrocarbons in mosses

Contact Info

Product

Resources

About