Reconstructed Skin to Create In Vitro Flexible Models of Skin Aging: New Results and Prospects

Asselineau, Daniel; Ricois, Sylvie; Piégay, Hervé; Zucchi, Hélène; Girardeau‐Hubert, Sarah; Deneuville, Céline; Haydont, Valérie; Neiveyans, Véronique; Lorthois, Isabelle

doi:10.1007/978-3-662-47398-6_48

Cited by 3 publications

(3 citation statements)

References 48 publications

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“…Indeed, two other full-thickness models, PhenionFT and EpidermFT, have been used for different applications, including: environmental and age-dependent effects [12,13,14]; skin penetration [15,16]; ultraviolet (UV) irradiation effects [17,18,19]; skin metabolism [20]; genotoxicity [21,22]; wound healing [23,24,25]; disease mechanisms [26] and skin sensitization [27]. Using the technology initially developed for research purposes [28], we have adapted the process for mass production of a new full thickness skin model, namely T-Skin™ (“T” refers to “total”, formerly RealSkin), according to ISO9001 standards to achieve reproducibility. The industrial-scale production of this reliable full-thickness model will allow for routine testing, as well as the development of research applications in academic and industrial scientific communities.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a New Reconstructed Full Thickness Skin Model, T-Skin™, and its Application for Investigations of Anti-Aging Compounds

Bataillon¹,

Lelièvre²,

Chapuis³

et al. 2019

IJMS

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Background: We have characterized a new reconstructed full-thickness skin model, T-Skin™, compared to normal human skin (NHS) and evaluated its use in testing anti-aging compounds. Methods: The structure and layer-specific markers were compared with NHS using histological and immunohistological staining. In anti-aging experiments, T-SkinTM was exposed to retinol (10 µM) or vitamin C (200 µM) for 5 days, followed by immunohistological staining evaluation. Results: T-Skin™ exhibits a well stratified, differentiated and self-renewing epidermis with a dermal compartment of functional fibroblasts. Epidermal (cytokeratin 10, transglutaminase 1), dermo–epidermal junction (DEJ) (laminin 5, collagen-IV, collagen VII) and dermally-located (fibrillin 1, procollagen I) biomarkers were similar to those in NHS. Treatment of T-Skin™ with retinol decreased the expression of differentiation markers, cytokeratin 10 and transglutaminase 1 and increased the proliferation marker, Ki67, in epidermis basal-layer cells. Vitamin C increased the expression of DEJ components, collagen IV and VII and dermal procollagen 1. Conclusions: T-Skin™ exhibits structural and biomarker location characteristics similar to NHS. Responses of T-Skin™ to retinol and vitamin C treatment were consistent with those of their known anti-aging effects. T-Skin™ is a promising model to investigate responses of epidermal, DEJ and dermal regions to new skin anti-ageing compounds.

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Section: Introductionmentioning

confidence: 99%

Characterization of a New Reconstructed Full Thickness Skin Model, T-Skin™, and its Application for Investigations of Anti-Aging Compounds

Bataillon¹,

Lelièvre²,

Chapuis³

et al. 2019

IJMS

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“…In the first, collagen is pre-exposed to UV radiations for the induction of dermal modifications. In the second, collagen is pre-incubated with sugar (ribose or glucose) prior use for preparing reconstructed skin [115].…”

Section: Skin Aging Modelmentioning

confidence: 99%

“…It has also been seen that by modifying the components of the ECM, the phenotype of fibroblasts can be changed. This is interesting to try to design a rejuvenation model of dermal fibroblast populations by modifying ECM [115].…”

Section: Skin Aging Modelmentioning

confidence: 99%

Bioengineered Skin Intended as In Vitro Model for Pharmacosmetics, Skin Disease Study and Environmental Skin Impact Analysis

et al. 2020

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This review aims to be an update of Bioengineered Artificial Skin Substitutes (BASS) applications. At the first moment, they were created as an attempt to replace native skin grafts transplantation. Nowadays, these in vitro models have been increasing and widening their application areas, becoming important tools for research. This study is focus on the ability to design in vitro BASS which have been demonstrated to be appropriate to develop new products in the cosmetic and pharmacology industry. Allowing to go deeper into the skin disease research, and to analyze the effects provoked by environmental stressful agents. The importance of BASS to replace animal experimentation is also highlighted. Furthermore, the BASS validation parameters approved by the OECD (Organisation for Economic Co-operation and Development) are also analyzed. This report presents an overview of the skin models applicable to skin research along with their design methods. Finally, the potential and limitations of the currently available BASS to supply the demands for disease modeling and pharmaceutical screening are discussed.

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Zidarič

Kleinschek²,

Maver³

et al. 2023

SpringerBriefs in Molecular Science

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Reconstructed Skin to Create In Vitro Flexible Models of Skin Aging: New Results and Prospects

Cited by 3 publications

References 48 publications

Characterization of a New Reconstructed Full Thickness Skin Model, T-Skin™, and its Application for Investigations of Anti-Aging Compounds

Characterization of a New Reconstructed Full Thickness Skin Model, T-Skin™, and its Application for Investigations of Anti-Aging Compounds

Bioengineered Skin Intended as In Vitro Model for Pharmacosmetics, Skin Disease Study and Environmental Skin Impact Analysis

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