Reconstitution of Pole‐to‐Pole Oscillations of Min Proteins in Microengineered Polydimethylsiloxane Compartments

Zieske, Katja; Schwille, Petra

doi:10.1002/anie.201207078

Cited by 98 publications

(150 citation statements)

References 24 publications

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“…Furthermore, the observed patterns lack standing wave dynamics with nodes where the timeaveraged local MinD concentration is minimum, as observed at midcell in vivo. Recently, standing wave dynamics were reconstituted by isolating the reaction in small volumes (30,31). However, the mechanistic basis for standing wave oscillation has not been experimentally addressed.…”

Section: Significancementioning

confidence: 99%

“…S3B). The MinE variant containing the MinD interaction interface but lacking both the dimerization and MTS domains, MinE [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] , stimulated the fastest MinD release rate and neither stabilized MinD nor lingered after MinD release (Fig. 6B and Fig.…”

Section: Mine Stabilizes Mind On the Membrane Before Stimulating Its mentioning

confidence: 99%

“…Recently, the Schwille group has shown that Min spirals on the bottom of an SLB-coated well are converted to an in vivo-like oscillating pattern (30,31). This was achieved by aspirating the sample solution so that the remaining volume was confined to 10-μm-deep × 10-μm-wide troughs with varying lengths (30,31) .…”

Section: Lingering Mine Suppresses Mind Membrane Binding and Establishesmentioning

confidence: 99%

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Membrane-bound MinDE complex acts as a toggle switch that drives Min oscillation coupled to cytoplasmic depletion of MinD

Vecchiarelli

Mizuuchi

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

119

227

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The Escherichia coli Min system self-organizes into a cell-pole to cell-pole oscillator on the membrane to prevent divisions at the cell poles. Reconstituting the Min system on a lipid bilayer has contributed to elucidating the oscillatory mechanism. However, previous in vitro patterns were attained with protein densities on the bilayer far in excess of those in vivo and failed to recapitulate the standing wave oscillations observed in vivo. Here we studied Min protein patterning at limiting MinD concentrations reflecting the in vivo conditions. We identified "burst" patterns-radially expanding and imploding binding zones of MinD, accompanied by a peripheral ring of MinE. Bursts share several features with the in vivo dynamics of the Min system including standing wave oscillations. Our data support a patterning mechanism whereby the MinD-to-MinE ratio on the membrane acts as a toggle switch: recruiting and stabilizing MinD on the membrane when the ratio is high and releasing MinD from the membrane when the ratio is low. Coupling this toggle switch behavior with MinD depletion from the cytoplasm drives a self-organized standing wave oscillator.cell division | subcellular organization | pattern formation | self-organization | intracellular positioning

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Section: Significancementioning

confidence: 99%

Section: Mine Stabilizes Mind On the Membrane Before Stimulating Its mentioning

confidence: 99%

Section: Lingering Mine Suppresses Mind Membrane Binding and Establishesmentioning

confidence: 99%

See 1 more Smart Citation

Membrane-bound MinDE complex acts as a toggle switch that drives Min oscillation coupled to cytoplasmic depletion of MinD

Vecchiarelli

Mizuuchi

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

119

227

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“…6a) (Zieske and Schwille 2013;Caspi et al 2014, to be published). On planar SLB, the reconstituted system forms running waves with a very long wavelength of several tens of microns [as well as other dynamical patterns (Ivanov and Mizuuchi 2010)], while in open micro-cavities it shows an oscillatory behavior similar to the in vivo one, albeit with the long in vitro wavelength (which is much longer than the in vivo length scale).…”

Section: Controlling the Position Of The Division Sitementioning

confidence: 99%

Divided we stand: splitting synthetic cells for their proliferation

Caspi

Dekker

2014

Syst Synth Biol

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With the recent dawn of synthetic biology, the old idea of man-made artificial life has gained renewed interest. In the context of a bottom-up approach, this entails the de novo construction of synthetic cells that can autonomously sustain themselves and proliferate. Reproduction of a synthetic cell involves the synthesis of its inner content, replication of its information module, and growth and division of its shell. Theoretical and experimental analysis of natural cells shows that, whereas the core synthesis machinery of the information module is highly conserved, a wide range of solutions have been realized in order to accomplish division. It is therefore to be expected that there are multiple ways to engineer division of synthetic cells. Here we survey the field and review potential routes that can be explored to accomplish the division of bottom-up designed synthetic cells. We cover a range of complexities from simple abiotic mechanisms involving splitting of lipid-membrane-encapsulated vesicles due to physical or chemical principles, to potential division mechanisms of synthetic cells that are based on prokaryotic division machineries.

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“…Petra Schwille presented reconstitution experiments with bacterial Min proteins on model membranes. This self-organizing system yielded travelling waves on large domains (Loose et al, 2008) and produced oscillations in closed compartments (Zieske and Schwille, 2013). The protein concentrations and kinetics were all measured (Loose et al, 2008;Loose et al, 2011).…”

Section: Emergence Of Patternsmentioning

confidence: 99%

Studies of morphogens: keep calm and carry on

Stathopoulos

Iber

2013

Development

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SummaryMorphogens are signaling factors that direct cell fate and tissue development at a distance from their source, and various modes of transport and interpretation have been suggested for morphogens. The recent EMBO Workshop on 'Morphogen gradients', which took place in Oxford, UK in June 2013, centered on the formation and interpretation of such morphogen gradients during development. This meeting allowed an exchange of views in light of recent results. Here, we provide a brief overview of the talks, organized in relation to several major themes of discussion at the meeting: (1) morphogen gradient formation; (2) morphogen gradient interpretation; (3) signaling networks and feedback in morphogenesis; (4) emergence of patterns; (5) scaling of patterns; (6) the control of growth; and (7) new techniques in the field.

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Reconstitution of Pole‐to‐Pole Oscillations of Min Proteins in Microengineered Polydimethylsiloxane Compartments

Abstract: Cell division in bacteria is highly regulated in time and space. The use of micrometer-sized sample volumes and model membranes allows the pole-to-pole oscillations of spatial regulators for bacterial cell division to be reconstituted in a synthetic minimal system.

Cited by 98 publications

References 24 publications

Membrane-bound MinDE complex acts as a toggle switch that drives Min oscillation coupled to cytoplasmic depletion of MinD

Membrane-bound MinDE complex acts as a toggle switch that drives Min oscillation coupled to cytoplasmic depletion of MinD

Divided we stand: splitting synthetic cells for their proliferation

Studies of morphogens: keep calm and carry on

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