Reconstitution of Excitable Cell Membrane Structure in Vitro

Müeller, Paul; Rudin, Donald O.; Tien, H. Ti; Wescott, William C.

doi:10.1161/01.cir.26.5.1167

Cited by 310 publications

(116 citation statements)

References 9 publications

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“…Phospholipids were dissolved in nheptane to a nal concentration of 20 mg/ml. BLM was formed by the procedure of Mueller et al (13) on an aperture in a cylindrical Te on chamber placed in a 12-ml glass cuvette. The working surface of BLM was 0.12 mm 2 .…”

Section: Methodsmentioning

confidence: 99%

Study of F‐Actin Interaction with Planar and Liposomal Bilayer Phospholipid Membranes

et al. 2000

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SummaryInteraction of the cytoskeletal protein F-actin with planar bilayer lipid membrane (BLM) induced formation of single ionic channels in both NaCl and KCl bathing solutions. We also recorded noiselike high-current jumps with a mean conductivity of » 160 pS, which might represent the simultaneous opening and closing of several channels of lower conductivity. The ratio of cation to anion permeabilities (Pc/Pa) of the BLM with many channels in KCl was 26 § 2. Freeze-fracture electron microscopy revealed brillar-like structures on the hydrophobi c surfaces of liposomal membranes. We also observed some structural features giving evidence for the penetration of F-actin bers through an arti cial phospholipid membrane. We suggest that the F-actin/lipids complexes can transmit electric signals in synaptic and other intercellular contacts.

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Section: Methodsmentioning

confidence: 99%

Study of F‐Actin Interaction with Planar and Liposomal Bilayer Phospholipid Membranes

et al. 2000

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“…However, the magnitude of the selectivity for K + over Na + was much less with PV (10-102) than it was with val (103-104) (9). For an unmodified bilayer, the I-V characteristic was linear over the range -I120 mV (16)(17)(18)(19). However, at all concentrations of PV tested, the I-V curves were nonlinear and I approached a maximum value (Im.x) independent of V when V exceeded 100 mV (Fig.…”

mentioning

confidence: 86%

Effect of Peptide PV on the Ionic Permeability of Lipid Bilayer Membranes

Ting‐Beall

Tosteson

Gisin

et al. 1974

The Journal of General Physiology

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This paper reports the effects of peptide PV (primary structure:cyclo-(D-val-L-pro-L-val-D-pro)3) on the electrical properties of sheep red cell lipid bilayers. The membrane conductance (Gm) induced by PV in either Na+ or K+ medium is proportional to the concentration of PV in the aqueous phase. The PV concentration required to produce a comparable increase in Gm in K+ medium is about 104 times greater than for its analogue, valinomycin (val). Although the selectivity sequence for PV and val is similar, K + > Rb + > Cs + > NH4 > T1+ > Na + > Li+; the ratio of G, in K + to that in Na + is about 10 for PV compared to > 103 for val. When equal concentrations of PV are added to both sides of a bilayer, the membrane current approaches a maximum value independent of voltage when the membrane potential exceeds 100 mV. When PV is added to only one side of a bilayer separating identical salt solutions of either Na+ or K+ salts, rectification occurs such that the positive current flows more easily away rather than toward the side containing the carrier. Under these conditions, a large, stable, zero-current potential (Vm) is also observed, with the side containing PV being negative. The magnitude of this Vm is about 90 mV and relatively independent of PV concentration when the latter is larger than 2 X 10-6 M. From a model which assumes that Vm equals the equilibrium potential for the PV-cation complexes (MS+) and that the reaction between PV and cations is at equilibrium on the two membrane surfaces, we compute the permeability of the membrane to free PV to be about 10-6 cm s', which is about 10 -7 times the permeability of similar membranes to free val. This interpretation is supported by the fact that the observed values of Vm are in agreement with the calculated equilibrium potential for MS+ over a wide range of ratios of concentrations of total PV in the two bathing solutions, if the unstirred layers are taken into account in computing the MS+ concentrations at the membrane surfaces.

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“…Inspired by these studies, in the early 1960's, Müller, Rudin, Tien and Wescott presented a way to prepare freestanding lipid bilayers, which are bordered by two aqueous reservoirs [MRTW62b,MRTW62a] (see figure 1.1(a)). According to previous works in history they called their membranes "Black Lipid Membranes" (BLMs).…”

Section: The Lipid Bilayer Conceptmentioning

confidence: 99%

“…The preparation of planar free-standing, solvent-containing lipid membranes goes back to the work of Müller and Rudin [MRTW62b,MRTW62a]. The remaining organic solvent in this kind of reconstituted membranes might be con- …”

Section: Preparation Of Black Lipid Membranesmentioning

confidence: 99%