Reconsideration of S-genotype Assignments, and Discovery of a New Allele Based on S-RNase PCR-RFLPs in Japanese Pear Cultivars.

Castillo, Carlos; Takasaki, Takeshi; Saito, Toshihiro; Yoshimura, Yumi; Norioka, Shigemi; Nakanishi, Tetsu

doi:10.1270/jsbbs.51.5

Cited by 33 publications

(19 citation statements)

References 11 publications

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“…Castillo et al (2001b) confirmed that the assignment of S-genotypes by the CAPS (S 1 to S 7 ) system coincided with that by pollination experiments. Thus, the CAPS (S 1 to S 7 ) system was shown to be highly reliable for identifying the S-genotypes of cultivars with S 1 -to S 7 -haplotypes.…”

Section: ) Development Of S-genotyping Systems Using the Pistil S Genesupporting

confidence: 82%

“…The S-genotypes of 'Akaho', 'Tanzawa', 'Kimizukawase', 'Choju', and 'Kumoi' were previously determined by pollination experiments as S 1 S 2 , S 3 S 5 /S 3 S 4 , S 2 S 5 , S 2 S 5 , and S 3 S 4 , respectively (Ogaki, 1958;Terami et al, 1946;Yasunobu et al, 1977). Using the CAPS systems, the S-genotypes of 'Akaho', 'Tanzawa', 'Kimizukawase', 'Choju', and 'Kumoi' were reassigned as S 3 S 5 , S 4 S 5 , S 1 S 5 , S 1 S 5 , and S 1 S 3 , respectively (Castillo et al, 2001b;Okada et al, 2004). The reassigned S-genotypes of 'Akaho', 'Tanzawa', and 'Kumoi' were confirmed by pollination experiments (Castillo et al, 2001b;Okada et al, 2004).…”

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 90%

“…It was thought that these cultivars had other uncharacterized S-RNases. Castillo et al (2001b) amplified a new 436 bp SRNase fragment from 'Ichiharawase' (Fig. 1B, lane 5), 'Meigetsu', and 'Heiwa' ('Nijisseiki' × 'Ichiharawase') using the CAPS (S 1 to S 7 ) system.…”

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

“…Using the CAPS systems, the S-genotypes of 'Akaho', 'Tanzawa', 'Kimizukawase', 'Choju', and 'Kumoi' were reassigned as S 3 S 5 , S 4 S 5 , S 1 S 5 , S 1 S 5 , and S 1 S 3 , respectively (Castillo et al, 2001b;Okada et al, 2004). The reassigned S-genotypes of 'Akaho', 'Tanzawa', and 'Kumoi' were confirmed by pollination experiments (Castillo et al, 2001b;Okada et al, 2004). The reassigned S-genotypes of 'Kimizukawase' and 'Choju' ['Asahi' (S 4 S 5 ) × 'Kimizukawase'] were consistent with those according to the pedigree: (Hiratsuka et al, 1998;Kajiura and Sato, 1990).…”

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

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DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (<i>Pyrus pyrifolia</i> Nakai)

Okada

2015

Hortic J

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Japanese pear (Pyrus pyrifolia Nakai) is an important Rosaceous fruit tree in Japan. This species exhibits gametophytic self-incompatibility (GSI), which is controlled by a single S-locus with multiple S-haplotypes. Although GSI is a genetic mechanism to prevent inbreeding and promote outcrossing to maintain genetic diversity, it can be problematic in fruit trees because it causes unstable fruit set. Therefore, orchardists interplant Japanese pear with other lines as pollinizers or conduct artificial pollination to ensure fruit set. To achieve stable fruit production and to reduce or eliminate the need for artificial pollination, research on the GSI of Japanese pear has been conducted by pollination experiments and by characterization of selfcompatible (SC) mutants. Additionally, breeding programs have progressed to produce SC cultivars with high fruit quality. Recently, molecular analyses of GSI and SC mutants in Japanese pear have provided new information that is relevant to the stable fruit production and efficient breeding of Japanese pear. This review focuses on studies of the GSI of Japanese pear, and especially on the recent development of DNA markers for S-genotyping and marker-assisted selection of SC trees. In addition, the candidate genes controlling pollen S specificity and a model of the molecular mechanism of GSI in Japanese pear are described.

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Section: ) Development Of S-genotyping Systems Using the Pistil S Genesupporting

confidence: 82%

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 90%

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning

confidence: 99%

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DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (<i>Pyrus pyrifolia</i> Nakai)

Okada

2015

Hortic J

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“…Nine S-RNase alleles corresponding to S 1 to S 9 have been cloned from six cultivars (Castillo et al, 2002;Ishimizu et al, 1998;Norioka et al, 1996;. Based on the nucleotide sequences of S 1 -to S 9 -RNases, cleaved amplified polymorphic sequence (CAPS) marker systems have been established for rapid S-genotyping (Ishimizu et al, 1999;Takasaki et al, 2004), and have assigned S-genotypes of 79 cultivars and selection lines (Castillo et al, 2001;Ishimizu et al, 1999;Kim et al, 2007;Okada et al, 2004;Sawamura et al, 2002;Takasaki et al, 2004). These results show that the S-genotypes of the recent cultivars consist of various pairs of only nine S-alleles (S 1 to S 9 ).…”

Section: Introductionmentioning

confidence: 99%

S-genotype Assignments of Local Cultivars in Japanese Pear ‘Senryo’, ‘Kuroki’, and ‘Hogyoku’

Okada

Castillo

Sawamura

et al. 2009

J. Japan. Soc. Hort. Sci.

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Japanese pear exhibits gametophytic self-incompatibility controlled by a single S-locus with multiple alleles. The S-locus encodes an S-RNase as a stylar product, and 10 S-RNase alleles (S 1 to S 9 , and S k ) have been cloned from major cultivars. To investigate the diversity of S-alleles in Japanese pear, we analyzed the S-genotypes of three local cultivars ('Senryo', 'Kuroki', and 'Hogyoku'). Two S-RNase fragments were amplified from each cultivar by genomic PCR with S-RNase-specific primers. A cleaved amplified polymorphic sequence (CAPS) marker system to distinguish S 1 -to S 9 -RNases assigned S 3 -and S 2 -RNase alleles to 'Senryo' and 'Kuroki', respectively. Cloning and sequencing of the other S-RNase genes identified S e -RNase of European pear in 'Senryo', S 12 -RNase of Chinese pear in 'Kuroki', and S 30 -RNase of Chinese pear and S k -RNase in 'Hogyoku'. Therefore, S-genotypes of 'Senryo', 'Kuroki', and 'Hogyoku' were assigned as S 3 S e , S 2 S 12 , and S k S 30 , respectively. These results revealed that Japanese pear has some of the same S-alleles as European and Chinese pears as well as S 1 -to S 9 -and S k -alleles.

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cDNA cloning of nine S alleles and establishment of a PCR-RFLP system for genotyping European pear cultivars

et al. 2006

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Nine full-length cDNAs of S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars by RT-PCR and 3' and 5' RACE. Comparison of the nucleotide sequences between the nine S-RNases cloned and 13 putative S alleles previously amplified by genomic PCRs revealed that seven corresponded to Sa, Sb, Sd, Se, Sh, Sk and Sl alleles, and the other two were new S alleles (designated as Sq and Sr alleles). Genomic PCR with a set of a8FTQQYQa9 and a8EP-anti-IIWPNVa9 primers was used to amplify nine S alleles; 1,414 bp (Sl), ca. 1.3 kb (Sk and Sq), 998 bp (Se), 440 bp (Sb) and ca. 350 bp (Sa, Sd, Sh and Sr). Among these, S alleles of similar size were discriminated by digestion with BaeI, BglII, BssHII, HindIII, EcoO109I and SphI. The PCR amplification of S allele following digestion with the restriction enzymes provided a PCR-RFLP system for rapid S-genotyping European pear cultivars harboring nine S alleles. The PCR-RFLP system assigned a total of 63 European pear cultivars to 25 genotypes. Among these, 14 genotypes were shared by two or more cultivars, which were cross-incompatible. These results suggested that the genes cloned represented the S-RNases from European pear, and that there were many cross-incompatible combinations among European pear varieties.

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Reconsideration of S-genotype Assignments, and Discovery of a New Allele Based on S-RNase PCR-RFLPs in Japanese Pear Cultivars.

Cited by 33 publications

References 11 publications

DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (<i>Pyrus pyrifolia</i> Nakai)

DNA Markers and the Molecular Mechanism of Self-(in)compatibility in Japanese Pear (<i>Pyrus pyrifolia</i> Nakai)

S-genotype Assignments of Local Cultivars in Japanese Pear ‘Senryo’, ‘Kuroki’, and ‘Hogyoku’

cDNA cloning of nine S alleles and establishment of a PCR-RFLP system for genotyping European pear cultivars

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