Recommendations of the International Council for Standardization in Haematology for Ethylenediaminetetraacetic Acid Anticoagulation of Blood for Blood Cell Counting and Sizing: International Council for Standardization in Haematology: Expert Panel on Cytometry

England, J. M.; Rowan, R. M.; Assendelft, Onno W. van; Bull, Brian S.; Coulter, Wallace H.; Fujimoto, Keiji; Groner, W.; Richardson-Jones, A; Klee, George G.; Koepke, John A.; Lewis, S. M.; McLaren, C. E.; Shinton, N. K.; Tatsumi, Noriyuki; Verwilghen, R. L.

doi:10.1093/ajcp/100.4.371

Cited by 106 publications

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“…It was reported that spuriously low WBC counts may be observed because of agglutination in the presence of EDTA [7]. Disodium, dipotassium, and tripotassium EDTA have been used for hematological testing [9]. In this study, dipotassium EDTA did not cause a change in WBC counts.…”

Section: Discussionmentioning

confidence: 60%

Changes of hematological references depends on storage period and temperature conditions in rats and dogs

Lee¹,

Kang

2016

Lab Anim Res

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Because changes in rat and dog hematological parameters according to storage conditions have been poorly documented, we sought to examine such changes. Blood analysis was performed using two hematology analyzers (ADVIA 2120i and Sysmex XN-V) after storage at room temperature and in cold storage for 5, 24, and 48 h, respectively. Interassay coefficients of variation for hematological parameters analyzed with the ADVIA 2120i and the XN-V showed similar. The levels of hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelet (PLT) showed significant variations with time in blood samples of rats and dogs. The leukocyte subpopulation showed high variation with storage conditions. The data for leukocyte differential counts obtained using the ADVIA 2120i, XN-V, and a manual differential counting procedure showed good agreement for neutrophils and lymphocyte counts, but monocytes, eosinophils, and basophils showed differences between the procedures. In conclusions, most rat and dog hematological parameters showed minimal changes; however, some showed high variation with storage time and temperature, especially PLT and leukocyte subpopulations. In conclusion, when performing hematological analysis in dogs and rats, it will be exactitude to analyze blood samples in fresh condition and at least within 24 h in the cold storage.

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Section: Discussionmentioning

confidence: 60%

Changes of hematological references depends on storage period and temperature conditions in rats and dogs

Lee¹,

Kang

2016

Lab Anim Res

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show abstract

“…Many other studies conducted in past conflicting results regarding findings of WBC, RBC and HGB at room temperature [5,9,12]. However, some studies, which were conducted using refrigerated temperature show that WBC, RBC, and HGB parameters, were constant up to 72 h [21,22].…”

Section: Discussionmentioning

confidence: 98%

“…Improved stability was observed in samples stored as K2-EDTA-anticogulated blood sample at refrigeration [5,9,10,[17][18][19]. Significant time and temperature related morphological alterations could happen with the long extended storage of blood on refrigeration (Figure 1) [17,20,21].…”

Section: Discussionmentioning

confidence: 99%

“…Influence of storage and variability on the majority of the blood count parameters can be minimized and if the analysis takes place before 24 h at 4°C [6,9,12,18]. …”

Section: Discussionmentioning

confidence: 99%

“…K2-EDTA having a less diluents effect and slighter influence on MCV, haematocrit, and effects on red blood cell size to increase the concentration, had been selected in our study over K3-EDTA [7,[9][10][11][12][13].…”

Section: Journal Of Clinical Research and Bioethicsmentioning

confidence: 99%

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