Recommendations for animal DNA forensic and identity testing

Budowle, Bruce; Garofano, Paolo; Hellman, Andreas; Ketchum, M.; Kanthaswamy, Sree; Parson, Walther; Haeringen, Wim van; Fain, Steve; Broad, T. E.

doi:10.1007/s00414-005-0545-9

Cited by 117 publications

(83 citation statements)

References 40 publications

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“…Gender was confirmed using an amelogenin marker (AMELY and AMELX) that was included in the Canine Genotypes TM Panel 1.1, F-860 S/L microsatellite panel (Finnzymes Diagnostics; Finnzymes OY, Keilaranta 16 A, 02150 Espoo, Finland). These microsatellites are included in the panel of loci by Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG) (Budowle et al, 2005). PCR amplification and electrophoresis (ABI Prism ® 310 Genetic Analyzer -Applied Biosystems) were carried out following the manufacturer's protocols.…”

Section: Samplingmentioning

confidence: 99%

Genetic characterization of four native Italian shepherd dog breeds and analysis of their relationship to cosmopolitan dog breeds using microsatellite markers

Bigi

Marelli

Randi

et al. 2015

Animal

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Very little research into genetic diversity of Italian native dog breeds has been carried out so far. In this study we aimed to estimate and compare the genetic diversity of four native Italian shepherd dog breeds: the Maremma, Bergamasco, Lupino del Gigante and Oropa shepherds. Therefore, some cosmopolitan dog breeds, which have been widely raised in Italy for a long time past, have also been considered to check possible influence of these dog populations on the Italian autochthonous breeds considered here. A total of 212 individuals, belonging to 10 different dog breeds, were sampled and genotyped using 18 autosomal microsatellite loci. We analyzed the genetic diversity of these breeds, within breed diversity, breed relationship and population structure. The 10 breeds considered in this study were clearly genetically differentiated from each other, regardless of current population sizes and the onset of separate breeding history. The level of genetic diversity explained 20% of the total genetic variation. The level of H E found here is in agreement with that found by other studies. The native Italian breeds showed generally higher genetic diversity compared with the long established, well-defined cosmopolitan dog breeds. As the Border Collie seems closer to the Italian breeds than the other cosmopolitan shepherd dogs considered here, a possible utilization of this breed to improve working performance in Italian traditional working shepherd dogs cannot be ignored. The data and information found here can be utilized in the organization of conservation programs planned to reduce inbreeding and to minimize loss of genetic variability.Keywords: genetic diversity, population genetic structure, domestic dog, shepherd dog, canine ImplicationsIn this study we aimed to estimate and compare the genetic diversity of four native Italian shepherd dog breeds and their relationship to cosmopolitan dog breeds reared in Italy. As these Italian autochthonous breeds are involved in sheepand cattle-farming in mountainous regions, they play a role in the economic tissue of marginal and disadvantaged areas, it is important to preserve their breeding. These data and information found here can be utilized in the organization of conservation programs planned to reduce inbreeding and to minimize loss of genetic variability. IntroductionThe dog is probably the most phenotypically diverse vertebrate species, thanks to its long history of domestication. Most of the various pure-breed dogs are morphologically clearly distinct and they also differ in behavior, physical properties and specific inherited diseases. Based on available breed histories, the majority of existing dog breeds were developed in the 19th century. At present, more than 400 canine breeds are registered worldwide by the Federation Cynologique Internationale (FCI, World Canine Organisation) and other federations such as the American and British Kennel Clubs (Parker et al., 2004;Streitberger et al., 2011).To understand the effect of breed structure on the genomic makeup of t...

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Section: Samplingmentioning

confidence: 99%

Genetic characterization of four native Italian shepherd dog breeds and analysis of their relationship to cosmopolitan dog breeds using microsatellite markers

Bigi

Marelli

Randi

et al. 2015

Animal

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“…The fragment coding for 28S presents regions variables, some of which are significantly different in size between diverse animal species [6,7,8,9,10,11].…”

Section: Discussionmentioning

confidence: 99%

A suspected case of hunting accident. Case report

Procaccianti¹,

Pascali²,

Triolo³

et al. 2010

J Forensic Res

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Nowadays, the use of molecular biology in forensics has made it possible to identify human victim and sometimes even the circumstances under which the death occurred through. In our case, a corpse of a 50-years old man with a gunshot wound was found in the woods.The suspected murderer declared that it had been a hunting accident while he shot a wild boar. During the autopsy, a bullet (Borra-bullet Gualandi, 32 gr) was found in the abdomen of the victim.

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“…Although, primarily due to the inherent heterogeneity of existing laboratory environments and the expense and complexity of accreditation, equalization of the nonhuman field to human forensic genetics poses a monumental challenge. Nonetheless, the first steps toward this goal must obviously be taken according to the developed recommendations concerning the standardization of DNA typing of animal species and products [270][271][272].…”

Section: Dna Analysis Of Nonhuman Remainsmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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Recommendations for animal DNA forensic and identity testing

Cited by 117 publications

References 40 publications

Genetic characterization of four native Italian shepherd dog breeds and analysis of their relationship to cosmopolitan dog breeds using microsatellite markers

Genetic characterization of four native Italian shepherd dog breeds and analysis of their relationship to cosmopolitan dog breeds using microsatellite markers

A suspected case of hunting accident. Case report

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

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