Recombination humanized type III collagen promotes oral ulcer healing

Abstract

ObjectiveRecombinant humanized type III collagen (rhCol III) is a highly adhesive biomaterial composed of 16 adhesion‐related tandem repeats refined from human type III collagen. Here, we aimed to investigate the effect of rhCol III on oral ulcers and reveal the underlying mechanism.MethodsAcid‐induced oral ulcers were induced on the murine tongue, and rhCol III or saline drops were administered. The effect of rhCol III on oral ulcers was assessed using gross and histological analyses. The effects on the proliferation, migration, and adhesion of human oral keratinocytes were investigated in vitro. The underlying mechanism was explored using RNA sequencing.ResultsAdministration of rhCol III accelerated the lesion closure of oral ulcers, reduced the release of inflammatory factors, and alleviated pain. rhCol III promoted the proliferation, migration, and adhesion of human oral keratinocytes in vitro. Mechanistically, the enrichment of genes associated with the Notch signaling pathway was upregulated after rhCol III treatment.ConclusionrhCol III promoted the healing of oral ulcers, showing promising therapeutic potential in oral clinics.

Tissue engineering applications of recombinant human collagen: a review of recent progress

“…Collagen possesses a characteristic triple-helical structure, composed of three polypeptide chains known as α-chains ( Mi et al, 2018 ; Rappu et al, 2019 ). In vitro studies have recombinantly produced different types of collagens from isolated genes, including types I, II, III, and V collagen ( Woodley et al, 2017 ; Doan et al, 2019 ; Shuai et al, 2023 ). Compared to natural collagen, recombinantly produced collagen achieves proper post-translational modifications, including hydroxylation and glycosylation ( Shekhter et al, 2019 ; Deng et al, 2021 ).…”

Injectable hydrogels of recombinant human collagen type III and chitosan with antibacterial and antioxidative activities for wound healing

“…These rhCol III exhibits similar properties and stability as natural human collagens, and offers several advantages including ease of obtainment, good water solubility, low immunogenicity, absence of hidden virus risks, and a stable production process. 22,23 It has drawn a lot of interest from the food, cosmetics, and pharmaceutical industries, [24][25][26][27][28] and has been investigated as bioactive materials in various tissue regeneration. [29][30][31][32][33] It is also reported that rhCol III was highly effective for wound healing.…”