Recombination between ten markers in Phycomyces

Alvarez, M. I.; Peláez, María Isabel; Eslava, Arturo P.

doi:10.1007/bf00425475

Cited by 26 publications

(8 citation statements)

References 15 publications

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“…A34 also carries a mutation in the madD gene required for phototropic response. The phototropism mutation and resistance to 5-FU were tightly linked, consistent with the previous segregation data for these two genes [27], [28]. The mating type was determined both by phenotype and by PCR amplification of the sex alleles, showing independent assortment of the sex locus with the furA and madD genes.…”

Section: Resultssupporting

confidence: 88%

“…Whatever the mechanism(s) of recombination in the zygospore, individual genes could then be defined into complementation groups and their relative placement on chromosomes established through genetic linkage analysis. Over a two decade period this process led to the measurement of linkage distances based on segregation between these markers [20], [21], [22], [23], [24], [25], [26], including centromeres that were mapped with the equivalent of tetrad analysis [27], [28]. The first genetic map was reported in 1987 [28], and the updated map was published in 1996 [29].…”

Section: Introductionmentioning

confidence: 99%

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A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus

et al. 2013

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Phycomyces blakesleeanus is a member of the subphylum Mucoromycotina. A genetic map was constructed from 121 progeny of a cross between two wild type isolates of P. blakesleeanus with 134 markers. The markers were mostly PCR-RFLPs. Markers were located on 46 scaffolds of the genome sequence, covering more than 97% of the genome. Analysis of the alleles in the progeny revealed nine or 12 linkage groups, depending on the log of the odds (LOD) score, across 1583.4 cM at LOD 5. The linkage groups were overlaid on previous mapping data from crosses between mutants, aided by new identification of the mutations in primary metabolism mutant strains. The molecular marker map, the phenotype map and the genome sequence are overall congruent, with some exceptions. The new genetic map provides a genome-wide estimate for recombination, with the average of 33.2 kb per cM. This frequency is one piece of evidence for meiosis during zygospore development in Mucoromycotina species. At the same time as meiosis, transmission of non-recombinant chromosomes is also evident in the mating process in Phycomyces. The new map provides scaffold ordering for the genome sequence and a platform upon which to identify the genes in mutants that are affected in traits of interest, such as carotene biosynthesis, phototropism or gravitropism, using positional cloning.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus

et al. 2013

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“…Crosses between sexually compatible mutants of P. blakesleeanus including 10 different genetic markers provided evidence for a single locus determining the mating type on a single linkage group together with the madE gene (Alvarez et al, 1980). The total genome sequence of this organism prompted Idnurm et al (2008) to look for this locus at the DNA level, based on analogy with mat-locus structures from other fungi.…”

Section: Discussionmentioning

confidence: 99%

“…Mating type loci, as in asco-and basidiomycetes, were not identified. For Phycomyces blakesleeanus, it is known, however, that the ability to mate as (+) or (2) can be mapped genetically to a single linkage group, presumably a locus (Alvarez et al, 1980). Based essentially on this phenotypic genetic mapping in P. blakesleeanus, Idnurm et al (2008) identified, at the molecular level, a DNA region that corresponds to the sex locus and that, with respect to the structure of the transcription factors encoded here, bears strong similarities to Mat-loci in ascomycetes (for a review see Pöggeler, 2001).…”

Section: Introductionmentioning

confidence: 99%

The mating-related loci sexM and sexP of the zygomycetous fungus Mucor mucedo and their transcriptional regulation by trisporoid pheromones

et al. 2012

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The putative mating type locus of mucoralean fungi consists of a single high mobility group (HMG)-domain transcription factor gene, sexM or sexP, flanked by genes for an RNA helicase and a triosephosphate transporter. We used degenerate primers derived from the amino acid sequence of the RNA helicase to sequence a fragment of this gene from Mucor mucedo. This fragment was extended by inverse PCR to obtain the complete sequences of the sex loci from both mating types of M. mucedo. The sex loci in M. mucedo reflect the general picture obtained previously for Phycomyces blakesleeanus, presenting a single HMG-domain transcription factor gene, sexM and sexP in the minus and plus mating types, respectively. These are located next to a gene for RNA helicase. Transcriptional analysis by quantitative real-time PCR showed that only transcription of sexM is considerably stimulated by adding trisporoid pheromones, thus mimicking sexual stimulation, whereas sexP is only slightly affected. These differences in regulation between sexM and sexP are supported by the observation that the promoter sequences controlling these genes show no similarities. The protein structures themselves are considerably different. The SexM, but not the SexP protein harbours a nuclear localization sequence. The SexM protein is indeed transported to nuclei. This was shown by means of a GFP fusion construct that was used to study the localization of SexM in the yeast Saccharomyces cerevisiae. The fusion protein is highly enriched in nuclei.

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“…On the other hand, chlorate resistant mutants, which are both nit1 (identical to niaD) and nitM (identical to one of cnx genes) in Glomerella graminicola Politis (Vaillancourt and Hanau, 1994) and Fusarium pone (Peck) Wollenweber (Liu and Sundheim, 1996), are recessive, as shown by a complementary heterokaryon test. In Phycomyces, only one mutant so far studied has been isolated that is resistant to 5-fluorouracil (Alvarez et al, 1980), showing a nucleous inherited dominant mutation. This resistance may be explained as being due to the Type 2 mechanism described in the Introduction (Hilgenberg et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of chlorate resistant mutants from nitrate-nonutilizing fungus Phycomyces blakesleeanus

2000

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Chlorate resistant mutants, which were first isolated in the zygomycetous fungus Phycomyces blakesleeanus, were found to be resistant up to a concentration of at least 300 mM of potassium chlorate. The dose-response relationship showed that although the mutants could be divided into two groups based on chlorate resistance in the mycelial elongation assay on the solid minimal medium, this was not observed in the assay using liquid culture. Genetic analysis of heterokaryons revealed the mutant alleles to be dominant. Enzymatic activities of three nitrate reductases and chlorate reductase were deficient in both the parent strain and the mutants. Intracellular incorporation of chlorate ion varied from strain to strain; however, the variation could not explain the mechanism of chlorate resistance. One unexpected characteristic of the mutants was that the intracellular sulfate ion concentration was 3.5 to 5.5 times higher than in the parent strain. We designated this mutant genotype crw, chlorate resistant mutant from nitrate-nonutilizing wild type.

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Recombination between ten markers in Phycomyces

Cited by 26 publications

References 15 publications

A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus

A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus

The mating-related loci sexM and sexP of the zygomycetous fungus Mucor mucedo and their transcriptional regulation by trisporoid pheromones

Isolation and characterization of chlorate resistant mutants from nitrate-nonutilizing fungus Phycomyces blakesleeanus

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