Recombination

Clark, David P.; Pazdernik, Nanette J.; McGehee, Michelle R.

doi:10.1016/b978-0-12-813288-3.00027-6

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…A thermostable DNA polymerase enzyme copies the target DNA in PCR in the presence of nucleotides and specific primers. The target DNA is exponentially amplified by repeated heating and cooling cycles in a thermocycler that results in rounds of target DNA denaturation, primer hybridisation and primer extension (Clark et al, 2019). PCR is an exemplar detection assay and is required for the initial diagnosis of a disease.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Molecular diagnostics in sericulture: A paradigm shift towards disease diagnosis in silkworms

Deepika,

Ramesh,

Kumar

et al. 2024

Entomologia Exp Applicata

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Sericulture, the practice of rearing silkworms for the production of silk, is an essential agro‐based industry in several countries. However, silkworms are susceptible to a variety of diseases caused by viruses, bacteria and parasites, which may have a significant negative impact on global silk production. Traditional methods of pathogen identification, such as microscopy and laboratory culturing, have limitations in terms of accuracy and efficiency. The development of molecular techniques for pathogen identification has revolutionised the field of sericulture over the last decade. Genomic DNA and RNA‐based molecular techniques allow for the rapid and accurate detection of disease‐causing pathogens in silkworms. Molecular diagnosis has several advantages over traditional methods, including increased sensitivity and specificity, shorter turnaround time and the ability to detect pathogens that are difficult to culture or visualise under a microscope. Molecular techniques have been applied to detect several important pathogens of silkworms, including Nosema sp., nucleopolyhedrovirus, cypovirus, iflavirus and bidensovirus. The use of molecular diagnostics in sericulture is immensely important as the demand for high‐quality silk increases globally and the assessment of emerging pathogens associated with crop loss is essential. Major advancements in the improvement and application of molecular methods for diagnosing widespread silkworm pathogens are discussed.

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Section: Polymerase Chain Reactionmentioning

confidence: 99%

Molecular diagnostics in sericulture: A paradigm shift towards disease diagnosis in silkworms

Deepika,

Ramesh,

Kumar

et al. 2024

Entomologia Exp Applicata

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“…1 For example, when two complementary strands polymerized from deoxyribonucleotides interact through thousands of hydrogen bonds, a DNA double helix structure is automatically generated to store and carry genetic information. 2 Right-handed B-DNA can invert to left-handed Z-DNA under the influence of external factors, thereby changing functions and roles. 3 Inspired by nature, chemists have developed a series of artificial chirality control systems based on external stimuli or more specifically supramolecular interactions.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Chirality control is of great significance to the normal progress of biological functions . For example, when two complementary strands polymerized from deoxyribonucleotides interact through thousands of hydrogen bonds, a DNA double helix structure is automatically generated to store and carry genetic information . Right-handed B-DNA can invert to left-handed Z-DNA under the influence of external factors, thereby changing functions and roles .…”

Section: Introductionmentioning

confidence: 99%

Adaptive Planar Chirality of Pillar[5]arenes Invertible by n-Alkane Lengths

Adachi

Wada

et al. 2023

J. Am. Chem. Soc.

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Chirality of host molecules can be induced and/or inverted by the guest molecules. However, the adapting chirality of hosts to the length of n-alkanes remains a great challenge because n-alkanes are neutral, achiral, and linear molecules, resulting in a weak interaction with most compounds. Herein, we report a system with chirality adapted to n-alkane lengths, using a pillar[5]arene-based macrocyclic host, S-Br, which contains five stereogenic carbons and five terminal bromine atoms on each rim. The electron-rich cavity of S-Br could include n-alkanes and the planar-chiral isomers sensitively inverted in response to the lengths of the complexed n-alkanes. The inclusion of a short n-alkane such as n-pentane made S-Br more inclined to be in the pS-form, whereas the inclusion of long n-alkanes such as n-heptane made the pR-form more favorable. The difference in the stability of the isomers was supported by the crystal structures and the theoretical calculations. Furthermore, temperature drives the adaptive chirality of S-Br with n-alkanes. An n-alkane with middle length, n-hexane, showed the dominance of the pR-form of S-Br at a higher temperature, whereas the pS-form was shown at a lower temperature.

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“…The eukaryotic nucleus is surrounded by the nuclear envelope (NE) and contains most of the cell’s genetic material in the form of chromosomes ( Clark et al, 2019 ; Webster et al, 2009 ). It represents the most prominent of several membrane-delimited organelles, each with its own specific and dynamic composition ( Cohen et al, 2018 ; Cole, 2016 ; Schrader et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

A general method for quantitative fractionation of mammalian cells

Udi

Zhang

Stein

et al. 2023

Journal of Cell Biology

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Subcellular fractionation in combination with mass spectrometry–based proteomics is a powerful tool to study localization of key proteins in health and disease. Here we offered a reliable and rapid method for mammalian cell fractionation, tuned for such proteomic analyses. This method proves readily applicable to different cell lines in which all the cellular contents are accounted for, while maintaining nuclear and nuclear envelope integrity. We demonstrated the method’s utility by quantifying the effects of a nuclear export inhibitor on nucleoplasmic and cytoplasmic proteomes.

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Recombination

Cited by 3 publications

References 10 publications

Molecular diagnostics in sericulture: A paradigm shift towards disease diagnosis in silkworms

Molecular diagnostics in sericulture: A paradigm shift towards disease diagnosis in silkworms

Adaptive Planar Chirality of Pillar[5]arenes Invertible by n-Alkane Lengths

A general method for quantitative fractionation of mammalian cells

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