Recombinants of PVY Strains Predominate Among Isolates from Potato Crop in Poland

Yin, Zhimin; Chrzanowska, M; Michalak, Krystyna; Zagórska, Helena; Zimnoch‐Guzowska, E.

doi:10.2478/v10045-012-0033-4

Cited by 17 publications

(14 citation statements)

References 31 publications

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“…60% were PVY N W. The proportions of these two recombinants in tested PVY infections differ considerably worldwide (Rigotti et al 2011, Yin et al 2012, Kamangar et al 2014. One reason seems to be differences in potato cultivar susceptibility to certain PVY strains, and therefore the ratio between PVY NTN and PVY N W/ N:O probably depends on which potato cultivars are favoured in different countries.…”

Section: Discussionmentioning

confidence: 98%

Potato virus Y (PVY) in Seed Potato Certification

et al. 2015

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In the EU and many other countries worldwide, seed potatoes require certification to be marketed. In most of the European national and international seed potato regulations, the tolerances for viruses are at present based on a combination of virus incidence and symptom severity. Historically all severe viruses were usually the origin of severe virus diseases, and mild viruses, in general, were seen to cause mild diseases. However, the situation has changed, especially for the most economically important virus, Potato virus Y (PVY), which is considered a severe virus. Investigation of PVY-infected potato leaf samples from Bavarian potato seed certification found that the "classical" O and the N strains, predominantly responsible for severe virus diseases, represented less than 1% of the infections present. Ca. 99% of the PVY infections were comprised of the new recombinant strains PVY NTN and PVY N W. The ratio between these recombinants differed substantially. Differences in susceptibility of particular cultivars to certain PVY strains seem to be one reason for that. Therefore, the ratio of the recombinants may depend upon which cultivars are favoured in different potato growing areas or countries.In greenhouse tests, PVY NTN and PVY N W were associated with both severe and mild disease symptoms. In corresponding field tests, the symptom severity differed considerably. Furthermore, there was no correlation detected between virus concentration and symptom severity.These results will contribute to a worldwide discussion on the elimination of the criterion virus symptom severity from potato seed certification during the forthcoming months. The discussion follows amendments made to international seed potato regulation documents. National regulations must be aligned by the EU member states until January 2016.

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Section: Discussionmentioning

confidence: 98%

Potato virus Y (PVY) in Seed Potato Certification

et al. 2015

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“…These symptoms were especially pronounced for isolates 2 and 4. Most European isolates of PVY N Wi cause only mild symptoms in potato (Chrzanowska 1991;Yin et al 2012). Very severe symptoms of virus infection of locally grown susceptible cultivars were also observed in the field in Vietnam: necrosis of veins, leaf fall and dark spots on leaves ('ink spots' or oak leaf necrotic pattern).…”

Section: Resultsmentioning

confidence: 99%

Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

Schubert

Thieme²,

Thieme

et al. 2014

Journal of Phytopathology

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Eight isolates from different potato growing regions in Vietnam were characterized. All were highly pathogenic in some potato cultivars, but did not overcome the extreme resistance of Solanum stoloniferum and Solanum demissum. RT‐PCR analysis revealed that all of these isolates are recombinants. Sequence data for 4 isolates were obtained, and their reaction in potato cultivars harbouring specific N genes was determined. Different phylogenetic analyses of viral sequences confirmed previous results that the recombinant isolates evolved from different parental sequences. One of the Vietnamese isolates investigated had a specific structure. The need for a clear classification of PVYNWi isolates is discussed.

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“…Moreover, the results demonstrate the occurrence of complete or at least partial cross‐protection among PVY isolates, with the NTN isolates being evidently more fit than their counterparts. The competitive advantage of the NTN isolates over the other isolates in mixed infections is an important factor that is responsible for the current predominance of these PVY forms in potato crops in many regions of the world (e.g., Yin et al ., ; Funke et al ., ). The competing strains showed a tendency towards separation from each other in host tissues.…”

Section: Discussionmentioning

confidence: 99%

Serological, molecular and immunofluorescent evidence for interference competition between isolates of Potato virus Y

2018

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Under natural field conditions, the abundance of plant viruses sharing the same host frequently results in mixed infections. Viruses co‐occurring inside host tissues often interact with one another synergistically or antagonistically. This paper addresses within‐host interactions among genetically different isolates of Potato virus Y (PVY), a serious pathogen that attacks solanaceous crops, particularly potato and tobacco. Manifold aspects of the relationships between six PVY isolates representing PVYO, PVYN‐Wi/PVYN:O and PVYNTN strains have been characterized. Plants of tobacco cv. Samsun and of two potato cultivars were inoculated both simultaneously and sequentially with different inoculation sequences and different time intervals between inoculations. Symptoms exhibited by doubly inoculated plants varied, primarily depending on the isolates involved as well as on the order of inoculation. Significant reductions of the titres of PVYN:O and PVYN‐Wi isolates in plants inoculated sequentially or simultaneously with PVYNTN isolates were recorded. These reductions were most probably due to inhibition of replication and accumulation of one isolate by another. In some combinations, a pre‐existing infection with PVYNTN prevented infection with the secondary virus. By contrast, challenge inoculation by PVYN:O, PVYN‐Wi or PVYO had no marked effects on the titre of the pre‐inoculated PVYNTN isolate. Microscopic visualization revealed a tendency of strains towards separation from each other in host tissues. These results provide strong experimental evidence for the existence of antagonistic interactions among PVY isolates. Moreover, these results demonstrate the occurrence of complete or at least partial cross‐protection among PVY isolates, with NTN isolates being evidently more fit than their counterparts.

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Recombinants of PVY Strains Predominate Among Isolates from Potato Crop in Poland

Cited by 17 publications

References 31 publications

Potato virus Y (PVY) in Seed Potato Certification

Potato virus Y (PVY) in Seed Potato Certification

Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

Serological, molecular and immunofluorescent evidence for interference competition between isolates of Potato virus Y

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