Recombinant β2-Glycoprotein I (β2GPI) Produced Using a Novel Lentiviral Approach Functions at Least As Well As Plasma-Derived β2GPI in Detection of Anti-β2GPI Antibodies
Abstract:Introduction
β2-glycoproteinI (β2GPI) is the primary antigen for antiphospholipid antibodies (Ab), and Ab to β2GPI are associated with thrombosis and recurrent fetal loss.β2GPIis comprised of 5 "sushi" domains. Complex disulfide bonding renders β2GPI a challenging protein to producerecombinantlyin high yield and most studies have utilized domain-deletion mutants produced on a lab scale for structure-function analyses.β2GPIalso has a complex tertiary structure, and is reported to circulate in a "… Show more
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