Recombinant vaccinia virus expression of the bovine leukaemia virus envelope gene and protection of immunized sheep against infection

Portetelle, Daniel; Limbach, Keith; Burny, Arsène; Mammerickx, Marc; Desmettre, P; Rivière, M.; Závada, Jan; Paoletti, Enzo

doi:10.1016/0264-410x(91)90153-w

Cited by 57 publications

(55 citation statements)

References 28 publications

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“…[91][92][93] Vaccination of mice and sheep using the rVV coding for BLV gp51 (BLVgp51-rVV) resulted in protective immunity, with the characteristics of a type 1 response. [94][95][96][97][98] These observations are in agreement with the notion that animals that control the virus express higher levels of interleukin-12 and interferon-gamma and lower levels of interleukin-10.…”

supporting

confidence: 78%

Bovine leukemia virus: current perspectives

Juliarena¹,

Barrios²,

Lützelschwab³

et al. 2017

VAAT

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Enzootic bovine leukosis, caused by bovine leukemia virus (BLV), is the most common neoplasm of dairy cattle. Although beef and dairy cattle are susceptible to BLV infection and BLV-associated lymphosarcoma, the disease is more commonly detected in dairy herds, mostly because of the management practices in dairy farms. The pathogenicity of BLV in its natural host, the bovine, depends mainly on the resistance/susceptibility genetics of the animal. The majority of infected cattle are asymptomatic, promoting the extremely high dissemination rate of BLV in many bovine populations. The important productive losses caused by the BLV, added to the health risk of maintaining populations with a high prevalence of infection with a retrovirus, generates the need to implement control measures. Different strategies to control the virus have been attempted. The most effective approach is to identify and cull the totality of infected cattle in the herd. However, this approach is not suitable for herds with high prevalence of infection. At present, no treatment or vaccine has proven effective for the control of BLV. Thus far, the genetic selection of resistant animals emerges as a natural strategy for the containment of the BLV dissemination. In natural conditions, most of the infected, resistant cattle can control the infection, and therefore do not pass the virus to other animals, gradually decreasing the prevalence of the herd.

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supporting

confidence: 78%

Bovine leukemia virus: current perspectives

Juliarena¹,

Barrios²,

Lützelschwab³

et al. 2017

VAAT

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“…Thus, resistance to BLV challenge after vaccination of sheep with envelope glycoprotein (Env) gp51 antigen and cattle with cells producing BLV envelope-encoded glycoproteins and p24 correlated with the presence of virusspecific antibodies (5,33). In contrast, cell-mediated immunity was the major protective mechanism in sheep inoculated with vaccinia virus expressing gp51 (14,31,32,36), and in which cytotoxic CD8 ϩ T-cell on gp51 were identified (13,16,24). However, the reason(s) why the progression of the disease and immune response in naturally infected animals are different has been unclear.…”

mentioning

confidence: 92%

The Influence of Ovine MHC Class IIDRB1Alleles on Immune Response in Bovine Leukemia Virus Infection

Konnai

Takeshima

Tajima

et al. 2003

Microbiology and Immunology

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We have reported previously that the alleles of the ovine leukocyte antigen (OLA)‐DRB1 gene that encode the Arg‐Lys (RK) motif and the Ser‐Arg (SR) motif at positions β70/71 of the OLA‐DRβ1 domain are associated with resistance and susceptibility, respectively, to development of bovine leukemia virus (BLV)‐induced ovine lymphoma. Here, to investigate the different immune response in sheep that carried alleles associated with resistance and susceptible for 30 weeks after infection with BLV, we selected sheep that had the RK/RK or SR/SR genotype among the 52 sheep analyzed by polymerase chain reaction‐restriction fragment length polymorphism and DNA sequencing of PCR product for the OLA‐DRB1 exon 2 and infected them with BLV. Although the number of BLV‐infected cells and virus titer had been maintaining low levels throughout the experimental period, the sheep with the RK/RK genotype could induce expansion of CD5− B‐cells and rapid production of neutralizing antibody in the early phase of infection. The level of incorporation of [3H]thymidine by peripheral blood mononuclear cells from the sheep with RK/RK genotype gave a strong response to BLV virion antigen and synthetic antigenic peptides that corresponded to T‐helper epitope of the BLV envelope glycoprotein gp51. In contrast, the sheep with SR/SR genotype showed a strong response to BLV virion antigen and synthetic antigenic peptides that corresponded to T‐cytotoxic and B‐cell epitopes. In such cases, the animals with the RK/RK strongly expressed IFN‐7, the animals with SR/SR genotype strongly expressed IL‐2. To determine the proliferating cells, we tried a blocking assay with monoclonal antibodies such as anti‐CD4, ‐CD8 and ‐DR molecule. We found that these proliferating cells were MHC‐restricted CD4+ T‐cells.

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“…Ainsi, le vaccin vaccine-Env du BLV (bovine leukemia virus) entraîne une protection chez les ovins (espèce sensible) mais pas chez les bovins, hôtes naturels du virus [10,11] que lorsque le taux de réplication virale dépasse un seuil donné. En dessous de ce seuil la réplication n'entraîne pas de maladie, même si le virus est totalement pathogène.…”

Section: Des Résultats Variablesunclassified

Vaccins rétroviraux : approche comparative en médecine humaine et vétérinaire

Dodet¹,

The²

1996

Médecine/Sciences

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Recombinant vaccinia virus expression of the bovine leukaemia virus envelope gene and protection of immunized sheep against infection

Cited by 57 publications

References 28 publications

Bovine leukemia virus: current perspectives

Bovine leukemia virus: current perspectives

The Influence of Ovine MHC Class IIDRB1Alleles on Immune Response in Bovine Leukemia Virus Infection

Vaccins rétroviraux : approche comparative en médecine humaine et vétérinaire

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