Recombinant Sendai Virus-mediated Gene Transfer into Adult Rat Retinal Tissue: Efficient Gene Transfer by Brief Exposure

Ikeda, Yasuhiro; Yonemitsu, Yoshikazu; Sakamoto, Taiji; Ishibashi, Tatsuro; Ueno, Hikaru; Kato, Atsushi; Nagai, Yoshinori; Fukumura, Masayuki; Inomata, Hajime; Hasegawa, Mamoru; Sueishi, Katsuo

doi:10.1006/exer.2002.1177

Cited by 28 publications

(33 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(i) SeV vectors can infect nondividing, quiescent cells as well as dividing cells unlike oncoretroviral vectors. [7][8][9][10][11] Thus, they can be used to infect cells that are terminally differentiated as well as at various stages of differentiation, whether they are dividing or not.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5] We have previously developed Sendai virus (SeV) vectors that replicate in the form of negative-sense single-stranded RNA in the cytoplasm of infected cells and do not go through a DNA phase. 6 SeV vectors can efficiently introduce foreign genes without toxicity into airway epithelial cells, 7 vascular tissue, 8 skeletal muscle, 9 synovial cells, 10 retinal tissue, 11 and hematopoietic progenitor cells. 12 Here we report that the SeV-mediated gene transfer into primate ES cells is very efficient and stable even after the terminal differentiation of the cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved

et al. 2004

View full text Add to dashboard Cite

Efficient gene transfer and regulated transgene expression in primate embryonic stem (ES) cells are highly desirable for future applications of the cells. In the present study, we have examined using the nonintegrating Sendai virus (SeV) vector to introduce the green fluorescent protein (GFP) gene into non-human primate cynomolgus ES cells. The GFP gene was vigorously and stably expressed in the cynomolgus ES cells for a year. The cells were able to form fluorescent teratomas when transplanted into immunodeficient mice. They were also able to differentiate into fluorescent embryoid bodies, neurons, and mature blood cells. In addition, the GFP expression levels were reduced dose-dependently by the addition of an anti-RNA virus drug, ribavirin, to the culture. Thus, SeV vector will be a useful tool for efficient gene transfer into primate ES cells and the method of using antiviral drugs should allow further investigation for regulated SeV-mediated gene expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved

et al. 2004

View full text Add to dashboard Cite

show abstract

“…30 Intraperitoneal injections of pentobarbital (1 mg/kg) were used to induce sufficient anesthesia. For all subretinal injections, we used an operating microscope to monitor related events.…”

Section: Gene Transfer Into Rat Retinamentioning

confidence: 99%

Simian immunodeficiency virus-based lentivirus vector for retinal gene transfer: a preclinical safety study in adult rats

et al. 2003

View full text Add to dashboard Cite

Although lentivirus vectors hold promise for ocular gene therapy, they also have potential safety issues, particularly in the case of the current human immunodeficiency virus-based vectors. We recently developed a novel lentivirus vector derived from the nonpathogenic simian immunodeficiency virus from African green monkeys (SIVagm) to minimize these potentials. In this preclinical study, we evaluated whether SIV vector could be efficiently and safely applicable to retinal gene transfer by assessing the transgene expression, retinal function and histology over a 1-year period following subretinal injection in adult rats. The functional assessment via electroretinogram after both titers of SIVlacZ (2.5 Â 10 7 or 2.5 Â 10 8 transducing units/ml) injection revealed both the dark and light adaptations to soon be impaired, in a dose-dependent manner, after a buffer injection as well, and all of them recovered to the control range by day 30. In both titers tested, the retinas demonstrated a frequent transgene expression mainly in the retinal pigment epithelium; however, the other retinal cells rarely expressed the transgene. Retinas exposed to a low titer virus showed no significant inflammatory reaction throughout the observation period, and also maintained the transgene expression over a 1-year period. In the retinas exposed to a high titer virus, however, mononuclear cell infiltration persisted in the subretinal area, and the retina that corresponded to the injected area finally underwent degeneration by around day 90. No retinal neoplastic lesions could be found in any animals over the 1-year period. We thus propose that SIV-mediated stable gene transfer might be useful for ocular gene transfer; however, more attention should be paid to avoiding complications when administering high titer lentivirus to the retina.

show abstract

“…[5][6][7][8][30][31][32][33] However, in our systems, excellent gene transfer could be achieved by the simple addition of SeV vector solution without specific reagents or centrifugation. Moreover, optimal SeV-mediated gene transfer to activated T cells could be performed during a relatively brief exposure time (less than 30 min, Figure 4d), with representative results seen in nasal mucosa, 20 in the vasculature, 21 in retinal tissue, 24 as well as in human umbilical cord bloodderived CD34-positive cells. 45 In addition, rare syncytium formation and few cytopathic effects were observed at the MOI used in this study (data not shown).…”

Section: Activated T-cell-directed Gene Transfer Via Sevmentioning

confidence: 99%

“…14 Using this vector, we demonstrated markedly superior gene transfer efficiency compared to findings with currently available vectors for use in various organs. [19][20][21][22][23][24][25] In contrast to systems involving retroviridae, replication and transcription of SeV are seen in the cytoplasm accompanying cellular tubulin, 26 and nuclear import and genomic integration are not required, 14 thereby suggesting the elimination of potential risks, including unexpected events related to chromosomal injury.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Sendai virus vectors for activated T lymphocytes

et al. 2003

View full text Add to dashboard Cite

Recombinant Sendai Virus-mediated Gene Transfer into Adult Rat Retinal Tissue: Efficient Gene Transfer by Brief Exposure

Cited by 28 publications

References 41 publications

Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved

Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved

Simian immunodeficiency virus-based lentivirus vector for retinal gene transfer: a preclinical safety study in adult rats

Recombinant Sendai virus vectors for activated T lymphocytes

Contact Info

Product

Resources

About