Recombinant Semliki Forest virus and Sindbis virus efficiently infect neurons in hippocampal slice cultures

Abstract

Gene transfer into nervous tissue is a powerful tool for the analysis of gene function. By using a rat hippocampal slice culture preparation, we show here that Semliki Forest virus (

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

“…2G-I) and axons ( Fig. 2J-L) in cultured pyramidal neurons as reported in vivo (Ehrengruber et al, 1999). Taken together, these findings strongly indicate that cortical pyramidal neurons in the dissociated cortical culture sort proteins, including 5-HT 2A receptors and major synaptic membrane proteins, in the same manner as seen in vivo.…”

LTP-triggered cholesterol redistribution activates Cdc42 and drives AMPA receptor synaptic delivery

“…This is a neurotropic plus-strand RNA virus, which acts as an mRNA inside the cell, leading to high protein expression with low toxicity for ≤72 h after infection (Ehrengruber et al, 1999;Malinow et al, 2010). Protein expression was 36-48 h for GluA1-EGFP and GluA2-EGFP and 12-24 h for EGFP-Rab11-DN and EGFP-Cdc42-DN.…”

Biology and application of alphaviruses in gene therapy

“…The first step towards gene therapy application was the demonstration of high transduction rates of various Large-scale production Drug screening, structural biology 9,10 Primary neurons Localization, electrophysiology 11 Hippocampal slices Localization, electrophysiology 12,13 In vivo, rat brain Localization, duration 16 Gene therapy Cancer vaccines Tumor protection, tumor regression 20 Tumor cell lines Transduction, cell killing 17,18 Intratumoral injection Tumor regression 23,24 Liposome-encapsulation Tumor-targeted gene delivery, therapy 28 18 Clearly, the infection rate was modest, although a bystander effect was observed for the therapeutic TK (thymidine kinase) after ganciclovir treatment. Another application of alphaviruses for cancer therapy has been vaccination with tumor antigens.…”