Recombinant <scp>DNA</scp> production of spider silk proteins

Tokareva, Olena; Michalczechen-Lacerda, V. A.; Rech, E. L.; Kaplan, David L.

doi:10.1111/1751-7915.12081

Cited by 155 publications

(154 citation statements)

References 63 publications

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“…The An and (GA)n motifs form β-sheet crystal structures to provide a high tensile strength and stiffness in the dragline silk. On the other hand, the GPGGX motifs form type II β-turn structures, and the GGX motif forms a 31-helix, both of which convey extensibility to the dragline fiber (Scheibel, 2004;Tokareva et al, 2013). With this understanding of the interplay between the sequence and the secondary structure properties, scientists have designed a series of spider silk-like polypeptides and peptides.…”

Section: Polypeptide/peptide Design Inspired By Silk Proteinsmentioning

confidence: 99%

“…Spider dragline silk is thought to be the toughest biological material yet identified; however, limitations on methods for mass production of spider silk have contributed to a gap between applications of silkworm silk and spider silk. Researchers recently developed a series of recombinant DNA technologies that enable spider silk production of the large scale (Tokareva et al, 2013). Recombinant technologies permit genetic modifications that can be imparted on the functionalities of spider silks in addition to their extraordinary mechanical properties (Schacht and Scheibel, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Cheng

Lee

2016

Front. Mater.

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Silks produced by spiders and silkworms are charming natural biological materials with highly optimized hierarchical structures and outstanding physicomechanical properties. The superior performance of silks relies on the integration of a unique protein sequence, a distinctive spinning process, and complex hierarchical structures. Silks have been prepared to form a variety of morphologies and are widely used in diverse applications, for example, in the textile industry, as drug delivery vehicles, and as tissue engineering scaffolds. This review presents an overview of the organization of natural silks, in which chemical and physical functions are optimized, as well as a range of new materials inspired by the desire to mimic natural silk structure and synthesis.

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Section: Polypeptide/peptide Design Inspired By Silk Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Cheng

Lee

2016

Front. Mater.

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“…Despite its clear potential, it is extremely difficult to obtain silk from spiders [56], and substantial research effort has been spent to produce spider-like silk at commercial scales using biomimetic approaches. While the properties of spider silk have not yet matched, the use of scalable techniques, advances in understanding of the structure and natural spinning of native silks and improvements in protein expression, have been driving the field closer to its goals [57][58][59][60].…”

Section: Spider Silk Fibers: a Leap In Materials Tribologymentioning

confidence: 99%

Advances in Bio-inspired Tribology for Engineering Applications

Siddaiah

Menezes

2016

J Bio Tribo Corros

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Bio-inspired tribology is an interdisciplinary field of science where scientists and engineers seek to investigate and incorporate tribological properties encountered in biological beings into engineering applications. In this paper, bio-inspired tribological research that are speculated to have a huge impact on tribological applications have been reviewed. These research involve (1) investigations related to replication of lubricin found in synovial fluids of mammalian joints which have super-low friction values that can be utilized in IC engines, (2) surface replication concerning to superhydrophobic properties of gecko skin which is seen to have anti-wetting and selfcleaning properties, (3) friction-reducing shark skin through specialized nanoparticle coatings that is seen to give a different perspective on surface texturing, (4) new techniques, such as soft lithography to replicate surfaces of lotus leaf and air lubrication phenomenon inspired by emperor penguins that is being applied to propel boats, ships, and torpedoes faster by reducing skin friction underwater. Further, an investigation in self-healing materials inspired from pitcher plant that has led to the innovation of self-healing and slippery liquid-infused porous surfaces has been discussed. These research works reviewed not only provide a deep insight into the current advances in bio-inspired tribology but also helps understand the plausibility of the research applications in the future and the practicality of innovations possible.

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“…9 and 52). The field has advanced substantially in recent years, 9 yet the success of synthetic silk development has been highly variable with no study demonstrating a synthetic fiber that reaches the property and processing benchmarks set by natural spider silks. This could be due to a range of factors including but not confined to limitations on the size of the gene inserted into the organism, limitations on the expression system of the recombinant organism, and a limited understanding of, and ability to replicate, the spider's natural spinning process.…”

Section: A Making Synthetic Silkmentioning

confidence: 99%

“…While we have not yet matched the properties of spider silk using scalable techniques, advances in our understanding of the structure and natural spinning of native silks, and improvements in protein expression, are driving the field closer to its goals. [6][7][8][9] …”

Section: Introductionmentioning

confidence: 99%

With great structure comes great functionality: Understanding and emulating spider silk

et al. 2014

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The overarching aim of biomimetic approaches to materials synthesis is to mimic simultaneously the structure and function of a natural material, in such a way that these functional properties can be systematically tailored and optimized. In the case of synthetic spider silk fibers, to date functionalities have largely focused on mechanical properties. A rapidly expanding body of literature documents this work, building on the emerging knowledge of structure-function relationships in native spider silks, and the spinning processes used to create them. Here, we describe some of the benchmark achievements reported until now, with a focus on the last five years. Progress in protein synthesis, notably the expression on full-size spidroins, has driven substantial improvements in synthetic spider silk performance. Spinning technology, however, lags behind and is a major limiting factor in biomimetic production. We also discuss applications for synthetic silk that primarily capitalize on its nonmechanical attributes, and that exploit the remarkable range of structures that can be formed from a synthetic silk feedstock.

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Recombinant DNA production of spider silk proteins

Cited by 155 publications

References 63 publications

Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Advances in Bio-inspired Tribology for Engineering Applications

With great structure comes great functionality: Understanding and emulating spider silk

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