Recombinant Protein Production and Purification of Insoluble Proteins

Ferrer-Miralles, Neus; Saccardo, Paolo; Corchero, José Luís; García‐Fruitós, Elena

doi:10.1007/978-1-0716-1859-2_1

Cited by 4 publications

(2 citation statements)

References 244 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The utilization of optimal conditions resulted in a protein extract of Chlorella pyrenoidosa with a concentration of 78% (wt/wt), however significant concentrations of ammonium sulfate and solvents were necessary for high protein yields. Reducing chemical inputs is critical for the application of this technology in commercial synthesis of microalgae proteins [146]. Protein purification and separation from microalgae, though complex and costly, yield high-purity proteins (90% to 98%) that find potential applications in health markets [147].…”

Section: Protein Extraction and Utilizationmentioning

confidence: 99%

Sustainable Biotechnology Approaches Using Chlorella vulgaris's Biomass and Lipids as Biofuel Feedstock

Singh,

Jain,

Dave

et al. 2024

Preprint

View full text Add to dashboard Cite

Chlorella vulgaris has considerable promise as a raw material for biofuel production since it has a high productivity per unit area, has a minimum influence on the environment, and does not significantly affect food security. Nevertheless, the low fatty content of their biomass poses a considerable economic obstacle for commercialization. Increasing the amount of biomass and lipids produced by these algae is essential for improving the economic feasibility of using them as biofuel sources. This review highlights the significance of identifying appropriate algal strains, namely those found in local environments, and utilising mutagenesis and genetic engineering techniques to create 'platform strains'. Prior endeavours have primarily concentrated on altering environmental and dietary parameters to enhance the production of biomass and lipids. Here, we review a scientific literature that examines biotechnological approaches and develops methodologies designed to increase lipid production, emphasising the importance of aligning engineering efforts with breakthroughs in DNA manipulation tools. In addition, the review research evaluates the present economic and commercial situation of algal biorefineries, including any related disadvantages. In summary, this thorough research highlights the importance of using creative methods to tackle the intricacies of lipogenesis and enhance the economic viability of producing biofuels from algae.

show abstract

Section: Protein Extraction and Utilizationmentioning

confidence: 99%

Sustainable Biotechnology Approaches Using Chlorella vulgaris's Biomass and Lipids as Biofuel Feedstock

Singh,

Jain,

Dave

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Although ALF Pm 3 could be expressed in P. pastoris , its antibacterial activity is affected by excessive glycosylation in P. pastoris [ 12 , 13 ]. With lots of advantages for industry, such as short growth cycle, posttranslational modification, low and cheap nutritional requirements, and efficient large-scale culture techniques [ 14 , 15 , 16 ], microalgae have been received widespread attention for their potential to be a factory to produce valuable components. Currently, studies on microalgae are mainly focused on Chlamydomonas reinhardtii , Chlorella vulgaris , Phaeodactylum tricornutum , and so on.…”

Section: Introductionmentioning

confidence: 99%

Comparing the Ability of Secretory Signal Peptides for Heterologous Expression of Anti-Lipopolysaccharide Factor 3 in Chlamydomonas reinhardtii

Zhuang

Chen

et al. 2023

Marine Drugs

View full text Add to dashboard Cite

Anti-lipopolysaccharide factor 3 (ALFPm3) possesses a wide antimicrobial spectrum and high antibacterial and viral activities for broad application prospects in the aquaculture industry. However, the application of ALFPm3 is limited by its low production in nature, as well as its low activity when expressed in Escherichia coli and yeast. Although it has been proven that its secretory expression can be used to produce antimicrobial peptides with strong antimicrobial activity, there is no study on the high-efficiency secretory expression of ALFPm3 in Chlamydomonas reinhardtii. In this study, signal peptides ARS1 and CAH1 were fused with ALFPm3 and inserted into the pESVH vector to construct pH-aALF and pH-cALF plasmids, respectively, that were transformed to C. reinhardtii JUV using the glass bead method. Subsequently, through antibiotic screening, DNA-PCR, and RT-PCR, transformants expressing ALFPm3 were confirmed and named T-JaA and T-JcA, respectively. The peptide ALFPm3 could be detected in algal cells and culture medium by immunoblot, meaning that ALFPm3 was successfully expressed in C. reinhardtii and secreted into the extracellular environment. Moreover, ALFPm3 extracts from the culture media of T-JaA and T-JcA showed significant inhibitory effects on the growth of V. harveyi, V. alginolyticus, V. anguillarum, and V. parahaemolyticus within 24 h. Interestingly, the inhibitory rate of c-ALFPm3 from T-JcA against four Vibrio was 2.77 to 6.23 times greater than that of a-ALFPm3 from T-JaA, indicating that the CAH1 signal peptide was more helpful in enhancing the secreted expression of the ALFPm3 peptide. Our results provided a new strategy for the secretory production of ALFPm3 with high antibacterial activity in C. reinhardtii, which could improve the application potentiality of ALFPm3 in the aquaculture industry.

show abstract

Molecular Farming for the Production of Recombinant Pharmaceutical Proteins in Plants

Fidan,

Secgin

2024

Concepts and Strategies in Plant Sciences

View full text Add to dashboard Cite

Recombinant Protein Production and Purification of Insoluble Proteins

Cited by 4 publications

References 244 publications

Sustainable Biotechnology Approaches Using Chlorella vulgaris's Biomass and Lipids as Biofuel Feedstock

Sustainable Biotechnology Approaches Using Chlorella vulgaris's Biomass and Lipids as Biofuel Feedstock

Comparing the Ability of Secretory Signal Peptides for Heterologous Expression of Anti-Lipopolysaccharide Factor 3 in Chlamydomonas reinhardtii

Molecular Farming for the Production of Recombinant Pharmaceutical Proteins in Plants

Contact Info

Product

Resources

About