Recombinant production of growth factors for application in cell culture

Venkatesan, Meenakshi; Semper, Cameron; Skrivergaard, Stig; DiLeo, Rosa; Mesa, Nathalie; Rasmussen, Martin Krøyer; Young, Jette F.; Therkildsen, Margrethe; Stogios, P.J.; Savchenko, Alexei

doi:10.1016/j.isci.2022.105054

Cited by 24 publications

(16 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, OPT13 contained an animal‐derived component, which is unsustainable. On the one hand, genetic engineering or microbial fermentation technology can be used to increase the yield of recombinant proteins and growth factors (Venkatesan et al., 2022), so as to control the cost in the future. On the other hand, safe and reliable food‐grade alternatives can be found, such as plant hydrolysates, yeast extracts, or food byproducts (Andreassen et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

Development of a serum‐free medium for myoblasts long‐term expansion and 3D culture for cell‐based meat

Dai,

Chen,

Xiong

et al. 2024

Journal of Food Science

View full text Add to dashboard Cite

Cell‐based meat technology provides an effective method to meet the demand for meat, while also posing a huge challenge to the expansion of myoblasts. It is difficult to develop serum‐free medium suitable for long‐term culture and large‐scale expansion of myoblasts, which causes limited understanding of myoblasts expansion. Therefore, this study used C2C12 myoblasts as model cells and developed a serum‐free medium for large‐scale expansion of myoblasts in vitro using the Plackett–Burman design. The serum‐free medium can support short‐term proliferation and long‐term passage of C2C12 myoblasts, while maintaining myogenic differentiation potential well, which is comparable to those of growth medium containing 10% fetal bovine serum. Based on the C2C12 myoblasts microcarriers serum‐free culture system established in this study, the actual expansion folds of myoblasts can reach 43.55 folds after 7 days. Moreover, cell‐based meat chunks were preliminarily prepared using glutamine transaminase and edible pigments. The research results provide reference for serum‐free culture and large‐scale expansion of myoblasts in vitro, laying the foundation for cell‐based meat production.Practical ApplicationThis study developed a serum‐free medium suitable for long‐term passage of myoblasts and established a microcarrier serum‐free culture system for myoblasts, which is expected to solve the problem of serum‐free culture and large‐scale expansion of myoblasts in cell culture meat production.

show abstract

Section: Resultsmentioning

confidence: 99%

Development of a serum‐free medium for myoblasts long‐term expansion and 3D culture for cell‐based meat

Dai,

Chen,

Xiong

et al. 2024

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…Growth Factors have gained increasing interest due to their critical roles in regenerative medicine, stem cell research, and more recently cellular agriculture (Venkatesan et al 2022). One of the most relevant growth factor for stem cell research is the basic Fibroblast Growth Factor (FGFb), which is widely used for culturing human embryonic stem cells (hESCs) (Levenstein et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Increasing interest has been directed towards recombinant growth factors due to their diverse applications, which include regenerative medicine, stem cell research and the emerging field of cell-culture meat production (Venkatesan et al 2022). In particular, the human basic Fibroblast Growth Factor (hFGFb) is a key cytokine which regulates cell proliferation and pluripotency and is required for the maintenance of mammalian cell cultures (Levenstein et al 2006; Lee et al 2012; Mossahebi-Mohammadi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Production of biologically active human basic Fibroblast Growth Factor (hFGFb) usingNicotiana tabacumtransplastomic plants

Müller,

Budnik,

Mirkin

et al. 2024

Preprint

View full text Add to dashboard Cite

The use of plants as biofactories presents as an attractive technology with the potential to efficiently produce high-value human recombinant proteins in a cost-effective manner. Plastid genome transformation stands out for its possibility to accumulate recombinant proteins at elevated levels. Of particular interest are recombinant growth factors, given their applications in animal cell culture and regenerative medicine. In this study we produced recombinant human Fibroblast Growth Factor (rhFGFb), a crucial protein required for animal cell culture, in tobacco chloroplasts. We successfully generated two independent transplastomic lines that are homoplasmic and accumulate rhFGFb in their leaves. Furthermore, the produced rhFGFb demonstrated its biological activity by inducing proliferation in HEK293T cell lines. These results collectively underscore plastid genome transformation as a promising plant-based bioreactor for rhFGFb production.

show abstract

“…Current edible scaffold approaches aim to grow cells on polysaccharide or protein-based materials such as cellulose and textured soy protein. , Developing such scaffolds requires the engineering of edible materials with favorable mechanical properties and texture which can be achieved through computational modeling. For serum-free media, efforts focus in two general categories: chemically defined media, with a focus on recombinant growth factors, and hydrolysates of food waste products to replace FBS. − Because computational modeling (for scaffold designs or media optimization) is currently commonly used in understanding material properties and interactions in cell culture applications, like predicting cell migration or alignment based on extracellular matrix stiffness or understanding collagen microfibril mechanical properties from atomistic scale up, computational modeling for cellular agriculture can rely on similar principles, with additional constraints on the materials …”

Section: Applicationsmentioning

confidence: 99%

Computational Design and Manufacturing of Sustainable Materials through First-Principles and Materiomics

et al. 2023

View full text Add to dashboard Cite

Engineered materials are ubiquitous throughout society and are critical to the development of modern technology, yet many current material systems are inexorably tied to widespread deterioration of ecological processes. Next-generation material systems can address goals of environmental sustainability by providing alternatives to fossil fuel-based materials and by reducing destructive extraction processes, energy costs, and accumulation of solid waste. However, development of sustainable materials faces several key challenges including investigation, processing, and architecting of new feedstocks that are often relatively mechanically weak, complex, and difficult to characterize or standardize. In this review paper, we outline a framework for examining sustainability in material systems and discuss how recent developments in modeling, machine learning, and other computational tools can aid the discovery of novel sustainable materials. We consider these through the lens of materiomics, an approach that considers material systems holistically by incorporating perspectives of all relevant scales, beginning with first-principles approaches and extending through the macroscale to consider sustainable material design from the bottom-up. We follow with an examination of how computational methods are currently applied to select examples of sustainable material development, with particular emphasis on bioinspired and biobased materials, and conclude with perspectives on opportunities and open challenges.

show abstract

Recombinant production of growth factors for application in cell culture

Cited by 24 publications

References 58 publications

Development of a serum‐free medium for myoblasts long‐term expansion and 3D culture for cell‐based meat

Development of a serum‐free medium for myoblasts long‐term expansion and 3D culture for cell‐based meat

Production of biologically active human basic Fibroblast Growth Factor (hFGFb) usingNicotiana tabacumtransplastomic plants

Computational Design and Manufacturing of Sustainable Materials through First-Principles and Materiomics

Contact Info

Product

Resources

About