Recombinant production and characterization of the carbohydrate recognition domain from Atlantic salmon C-type lectin receptor C (SCLRC)

Molecular characterization of a transmembrane C-type lectin receptor gene from ayu (Plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages

“…7B). This result was not unexpected because animal CTLs have a relaxed binding specificity (Soanes et al, 2008). The B max and K d values revealed that the binding capacity and rate of rPaCTLRC to d-mannose were higher than those of other monosaccharides (Table 3).…”

“…Moreover, PaCTLRC has eight repeats consistent with a coiled coil domain in the extracellular neck region (aa 124-165), suggesting that the protein is capable of oligomerization (Manzo et al, 2012). The Tyr (Y) 288 residue in SCLRC, which was identified to be the binding site of d-galactose (Soanes et al, 2008), was also existed in the PaCTLRC at aa position 304 with highly conserved amino acid sequences peripheral to the site (Fig. 1).…”

The Lectin Frontier Database (LfDB), and Data Generation Based on Frontal Affinity Chromatography

“…Xenopus galectin-VIIa [81] Human, chicken, nematode, sponge, fungal galectrins [36] Human galectin-9 [82] Nematode galectin LEC-1 and N-and C-CRDs [83] C-type PF00059 C-type α/β-fold Atlantic salmon serum [84] calcium-dependent Sea cucumber lectin CEL-IV [85] DC-SIGN, DC-SIGNR, and LSECtin [86] Langerin to sulfated and mannsylated glycans [87] MGL1, MGL2, and their mutants [88] Atlantic salmon C-type lectin receptor C (SCLRC) [89] Acorn barnacle Megabalanus rosa, Balanus rostatus [90] R-type PF00652 β-trefoil Mutants of earthworm lectin EW29Ch [91] ricin B chain-related RCA120/RCA-I [58] Phytopathogenic Sclerotinia sclerotiorum [92] Jacalin-related PF01419 β-prism I Human ZG16 p [93] Mannose-binding type Jacalin-related lectins [37] Jacalin [94] Castanea crenata agglutinin (CCA) [95] Cycas revoluta leaf lectin (CRLL) [95] GNA-related monocot-type PF04152 β-prism II Two-domain GNA-related lectins [96] Sea urchin egg PF02140 α/β-fold with two long Shishamo smelt Osmerus (Spirinchus) lanceolatus [97] Fungal fucose-specific PF07938 6-bladed β-propeller Mushroom Pholiota squarrosa [104] Aspergillus oryzae [105] AAL-like Fungal fruit body lectin PF07367 α/β-sandwich Agaricus bisporus agglutinin (ABA) [106] Others Mussel, Mytilus galloprovincialis [107] orphan or family Mushroom Hygrophorus russula [108] unidentified Eggs of Japanese sea hare Aplysia kurodai [109] Feather star Oxycomanthus japonicas [110] Coronate moon turban Turbo (Lunella)…”

Cystine-mediated oligomerization of the Atlantic salmon serum C-type lectin

“…Homology modelling followed a procedure similar to that used previously for another C-type CTLD [15]. The isoform used was SSL-2, which will hereafter be referred to as SSL except when otherwise specified.…”

“…SSL and its mutants were expressed in E. coli using the system previously described for the Atlantic salmon C-type lectin receptor C (SCLRC) [15]. Briefly, the cDNA fragment encoding the mature SSL-2 isoform was cloned in frame with a sequence encoding the PelB leader sequence and six-histidine-tag of the pET22b plasmid (Novagen, Madison, U.S.A.), allowing expression of rSSL as a fusion protein directed toward the periplasm.…”