Recombinant porcine Interferon-α and Interleukin-2 fusion protein (rPoIFNα+IL-2) shows potent anti-pseudorabies virus activity in vitro and in vivo

Zhao, Kuan; Li, Xiuli; Lei, Baishi; Han, Ying; An, Tongqing; Zhang, Wuchao; Zhang, Huiwen; Li, Bosen; Yuan, Wanzhe

doi:10.1016/j.vetmic.2023.109678

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Previous studies have shown that an H7N9 subunit vaccine designed using a combination of highly immunogenic epitopes and linkers could result in a strong immune response ( Hasan et al, 2019 ). Meanwhile, our study results suggested that a recombinant porcine IFN-α and IL-2 fusion protein ( rPoIFNα+IL-2 ) has both the antiviral effect of IFNα and the pro-proliferation effect of IL-2 ( Zhao et al, 2023 ). These results suggest that multiple epitopes or structural domains expressed in tandem play coordinating roles.…”

Section: Discussionmentioning

confidence: 87%

“…Subsequently, pCold-Fiber1/2-knob and pCold-Fiber2 were transferred into Escherichia coli BL21 separately for expression of 2 fusion recombinant proteins as previously described ( He et al, 2018 ). The protein was purified using Ni-agarose resin (Kangwei, Beijing, China) as previously described ( Zhao et al, 2023 ). The subunit vaccines were prepared by emulsifying recombinant proteins with Freund's complete adjuvant at a volume ratio 1:1 which the protein content was calculated as 100 μg/mL.…”

Section: Methodsmentioning

confidence: 99%

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A novel subunit vaccine based on Fiber1/2 knob domain provides full protection against fowl adenovirus serotype 4 and induces stronger immune responses than a Fiber2 subunit vaccine

Liu,

Dong,

Lei

et al. 2024

Poultry Science

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Section: Discussionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

A novel subunit vaccine based on Fiber1/2 knob domain provides full protection against fowl adenovirus serotype 4 and induces stronger immune responses than a Fiber2 subunit vaccine

Liu,

Dong,

Lei

et al. 2024

Poultry Science

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Integrated bioinformatics analysis of dendritic cells hub genes reveal potential early tuberculosis diagnostic markers

Wu,

Liu,

et al. 2023

BMC Med Genomics

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Background Dendritic cells (DCs) are most potent antigen-processing cells and play key roles in host defense against Mycobacterium tuberculosis (MTB) infection. In this study, hub genes in DCs during MTB infection were first investigated using bioinformatics approaches and further validated in Monocyte-derived DCs. Methods Microarray datasets were obtained from Gene Expression Omnibus (GEO) database. Principal component analysis (PCA) and immune infiltration analysis were performed to select suitable samples for further analysis. Differential analysis and functional enrichment analysis were conducted on DC samples, comparing live MTB-infected and non-infected (NI) groups. The CytoHubba plugin in Cytoscape was used to identify hub genes from the differentially expressed genes (DEGs). The expression of the hub genes was validated using two datasets and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in human monocyte-derived DCs. Enzyme-linked immunosorbent assay (ELISA) was used to validate interferon (IFN) secretion. Transcription factors (TFs) and microRNAs (miRNAs) that interact with the hub genes were predicted using prediction databases. The diagnostic value of the hub genes was evaluated using receiver operating characteristic (ROC) curves and area under the curve (AUC) values. Results A total of 1835 common DEGs among three comparison groups (18 h, 48 h, 72 h after MTB infection) were identified. Six DEGs (IFIT1, IFIT2, IFIT3, ISG15, MX1, and RSAD2) were determined as hub genes. Functions enrichment analysis revealed that all hub genes all related to IFN response. RT-qPCR showed that the expression levels of six hub genes were significantly increased after DC stimulated by live MTB. According to the results of ELISA, the secretion of IFN-γ, but not IFN-α/β, was upregulated in MTB-stimulated DCs. AUC values of six hub genes ranged from 84 to 94% and AUC values of 5 joint indicators of two hub genes were higher than the two hub genes alone. Conclusion The study identified 6 hub genes associated with IFN response pathway. These genes may serve as potential diagnostic biomarkers in tuberculosis (TB). The findings provide insights into the molecular mechanisms involved in the host immune response to MTB infection and highlight the diagnostic potential of these hub genes in TB.

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A pH-Triggered self-releaseing humic acids hydrogels loaded with porcine interferon α/γ achieves anti-PRV effects by oral administration

Sun,

Shi,

Lei

et al. 2024

Preprint

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Interferon α (IFNα) and interferon γ (IFNγ) are cytokines that mediate important biological functions, including antiviral activity and immune regulation. However, the function of monomer IFN was limited and the administration route completely depends on injection. To solve this problem, recombinant porcine IFN-α and IFN-γ fusion protein (rPoIFNα/γ) was expressed, purified, and used to develop an effective oral rPoIFNα/γ humic acid hydrogel delivery system triggered by pH to protect the IFNα/γ from gastric acid destruction. Neither the humic acid hydrogel nor rPoIFNα/γ showed cytotoxicity in vitro for porcine kidney-15 (PK-15) cells. rPoIFNα/γ inhibited the replication of vesicular stomatitis virus (VSV) and pseudorabies virus (PRV), with an antiviral activity of approximately 104 U/mL. Scanning electron microscopy revealed that the humic acid (HA) hydrogel had a loose and porous honeycomb structure. rPoIFNα/γ was adsorbed by the hydrogel (IFNα/γ@PAMgel) and measured using Fourier transform infrared spectroscopy, and the results indicated a good IFN-loading effect. In vitro experiments showed that IFNα/γ@PAMgel swelled and released the IFNα/γ rapidly at pH 7.4 but not at pH 1.2. Mice oral administered IFNα/γ@PAMgel had enhanced proliferation and differentiation of CD4+ and CD8+ cells, whereas mice infected with PRV and treated with IFNα/γ@PAMgel had increased interferon stimulating genes (ISGs) transcription levels in the serum, lower mortality, lower viral loads in different tissues, and lower levels of organ damage. Conclusively, this study demonstrates that oral administered IFNα/γ@PAMgel has antiviral and immunomodulatory effects and is a potential antiviral agent for PRV infection.

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Recombinant porcine Interferon-α and Interleukin-2 fusion protein (rPoIFNα+IL-2) shows potent anti-pseudorabies virus activity in vitro and in vivo

Cited by 3 publications

References 33 publications

A novel subunit vaccine based on Fiber1/2 knob domain provides full protection against fowl adenovirus serotype 4 and induces stronger immune responses than a Fiber2 subunit vaccine

A novel subunit vaccine based on Fiber1/2 knob domain provides full protection against fowl adenovirus serotype 4 and induces stronger immune responses than a Fiber2 subunit vaccine

Integrated bioinformatics analysis of dendritic cells hub genes reveal potential early tuberculosis diagnostic markers

A pH-Triggered self-releaseing humic acids hydrogels loaded with porcine interferon α/γ achieves anti-PRV effects by oral administration

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