Recombinant porcine interferon cocktail delays the onset and lessens the severity of African swine fever

Jiao, Peng; Wang, Shuchao; Fan, Wenhui; Zhang, He; Yin, Hui; Shang, Yue; Zhu, Hongfei; Liu, Wenjun; Hu, Rongliang; Sun, Li

doi:10.1016/j.antiviral.2023.105644

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…Quantifying the expression of IFN-γ and IL-4 in spleen lymphocytes stimulated by the eight ASFV antigens allowed determining the type of immune response induced by the multi-antigen recombinant SC strains. Th1 cells secrete IL-2, IFN-γ, TNF-α, and other cytokines, mediating cellular immune responses ( 72 ). Th2 cells secrete IL-4, IL-5, IL-6, IL-10, and other cytokines, mediating humoral immune responses ( 73 , 74 ).…”

Section: Discussionmentioning

confidence: 99%

Saccharomyces cerevisiae oral immunization in mice using multi-antigen of the African swine fever virus elicits a robust immune response

Gao,

Zuo,

Kang

et al. 2024

Front. Immunol.

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African swine fever virus (ASFV) is one of the most complex viruses. ASFV is a serious threat to the global swine industry because no commercial vaccines against this virus are currently available except in Vietnam. Moreover, ASFV is highly stable in the environment and can survive in water, feed, and aerosols for a long time. ASFV is transmitted through the digestive and respiratory tract. Mucosal immunity is the first line of defense against ASFV. Saccharomyces cerevisiae (SC), which has been certified by the U.S. Food and Drug Administration and has a generally recognized as safe status in the food industry, was used for oral immunization in this study. ASFV antigens were effectively expressed in recombinant SC strains with high DNA copy numbers and stable growth though surface display technology and chromosome engineering (δ-integration). The recombinant SC strains containing eight ASFV antigens—KP177R, E183L, E199L, CP204L, E248R, EP402R, B602L, and B646L— induced strong humoral and mucosal immune responses in mice. There was no antigenic competition, and these antigens induced Th1 and Th2 cellular immune responses. Therefore, the oral immunization strategy using recombinant SC strains containing multiple ASFV antigens demonstrate potential for future testing in swine, including challenge studies to evaluate its efficacy as a vaccine against ASFV.

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Section: Discussionmentioning

confidence: 99%

Saccharomyces cerevisiae oral immunization in mice using multi-antigen of the African swine fever virus elicits a robust immune response

Gao,

Zuo,

Kang

et al. 2024

Front. Immunol.

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“…Indeed, IFN-α/β orchestrate a multifaceted defense strategy by inducing an array of antiviral proteins, encoded by ISGs (Interferon stimulated genes), that inhibit viral replication and spread ( 22 ). The critical role of IFN-α/β for the establishment of an efficient antiviral response against ASFV has also been documented ( 23 , 24 ). Their synthesis are based on the recognition of specific molecular motifs known as PAMPs (pathogen-associated molecular patterns) by either membrane receptors or cytoplasmic sensors, collectively termed PRRs (pattern recognition receptors).…”

Section: Introductionmentioning

confidence: 99%

Exploring type I interferon pathway: virulent vs. attenuated strain of African swine fever virus revealing a novel function carried by MGF505-4R

Dupré,

Le Dimna,

Hutet

et al. 2024

Front. Immunol.

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African swine fever virus represents a significant reemerging threat to livestock populations, as its incidence and geographic distribution have surged over the past decade in Europe, Asia, and Caribbean, resulting in substantial socio-economic burdens and adverse effects on animal health and welfare. In a previous report, we described the protective properties of our newly thermo-attenuated strain (ASFV-989) in pigs against an experimental infection of its parental Georgia 2007/1 virulent strain. In this new study, our objective was to characterize the molecular mechanisms underlying the attenuation of ASFV-989. We first compared the activation of type I interferon pathway in response to ASFV-989 and Georgia 2007/1 infections, employing both in vivo and in vitro models. Expression of IFN-α was significantly increased in porcine alveolar macrophages infected with ASFV-989 while pigs infected with Georgia 2007/1 showed higher IFN-α than those infected by ASFV-989. We also used a medium-throughput transcriptomic approach to study the expression of viral genes by both strains, and identified several patterns of gene expression. Subsequently, we investigated whether proteins encoded by the eight genes deleted in ASFV-989 contribute to the modulation of the type I interferon signaling pathway. Using different strategies, we showed that MGF505-4R interfered with the induction of IFN-α/β pathway, likely through interaction with TRAF3. Altogether, our data reveal key differences between ASFV-989 and Georgia 2007/1 in their ability to control IFN-α/β signaling and provide molecular mechanisms underlying the role of MGF505-4R as a virulence factor.

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When ASFV Infection Meets the cGAS-STING Signaling Pathway

Chen,

Shang,

Wei

et al. 2024

Transboundary and Emerging Diseases

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The African swine fever virus (ASFV) has the ability to infect both wild boars and domestic pigs, regardless of their breeds or ages, often resulting in a mortality rate of 100%. Host innate immunity is the most important defense weapon against invasion of pathogenic microbial infection. cGAS-STING signaling pathway is one of the greatest discoveries of the twenty-first century, which is crucial in host’s innate immune response. Recent studies have found that the interaction between cGAS/STING pathway and ASFV plays a key role during ASFV infection. At the same time, ASFV has also evolved different strategies to evade the killing of the host cGAS/STING pathway and promote its survival. Here, we review the latest progress in the interaction between ASFV infection, cGAS/STING pathways, and their related molecular mechanisms, aiming to provide new ideas for further research on the pathogenesis of ASFV, the development of vaccines and therapeutic drugs.

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Recombinant porcine interferon cocktail delays the onset and lessens the severity of African swine fever

Cited by 4 publications

References 44 publications

Saccharomyces cerevisiae oral immunization in mice using multi-antigen of the African swine fever virus elicits a robust immune response

Saccharomyces cerevisiae oral immunization in mice using multi-antigen of the African swine fever virus elicits a robust immune response

Exploring type I interferon pathway: virulent vs. attenuated strain of African swine fever virus revealing a novel function carried by MGF505-4R

When ASFV Infection Meets the cGAS-STING Signaling Pathway

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