Recombinant Mycobacterium leprae protein associated with entry into mammalian cells of respiratory and skin components

Sato, Naoya; Fujimura, Takao; Masuzawa, Mikio; Yogi, Yasuko; Matsuoka, Masao; Kanoh, Maho; Riley, Lee W.; Katsuoka, Kensei

doi:10.1016/j.jdermsci.2007.01.006

Cited by 15 publications

(20 citation statements)

References 20 publications

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“…Briefly, it was grown in basal media (HuMedia‐EB2; Kurabo) supplemented with 5% fetal bovine serum (FBS; JRH Bioscience, Lenexa, KS, USA), 1 μg/mL hydrocortisone, 39.3 μg/mL dibutyryl cyclic adenosine monophosphate, 50 μg/mL amphotericin B, 10 ng/mL human epidermal growth factor receptor, 5 ng/mL human basic fibroblast growth factor and 10 μg/mL heparin. This study used the fifth passage of HMVEC …”

Section: Methodsmentioning

confidence: 99%

“…We also have reported that this protein is an important pathogenic factor involved in the invasion of M. leprae into nasal epithelial cells and human microvascular endothelial cells (HMVEC), and further, clarified that the active sequence of M. leprae involved in the invasion into nasal mucosa is present in the a.a. region of 316-531 (72 a.a. in the M. leprae InvX) of the mce1A region. 7,8 However, the molecular mechanism involved in the invasion of M. leprae into HMVEC has not been sufficiently studied. Therefore, this study was conducted to identify the invasion activity of the active sequence in the mce1A region into vascular endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

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Invasion of human microvascular endothelial cells by Mycobacterium leprae through Mce1A protein

Idris

Abdurrahman

Fatulrachman³

et al. 2019

The Journal of Dermatology

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In patients with lepromatous leprosy, Mycobacterium leprae is often observed inside the human microvascular endothelial cells (HMVEC) surrounding Schwann cells (SC) at the site of lesions in the peripheral nerves. Based on this observation, it is considered that the nasal mucous may be the invasion pathway for M. leprae and HMVEC serve as an important reservoir for the bacteria before they invade SC. In light of previous research which revealed that Mce1A protein mediates bacterial invasion into nasal epithelial cells and HMVEC, we conducted a study to determine whether the invasion of M. leprae into HMVEC can be suppressed by blocking the Mce1A protein. In this study, we analyzed bacterial invasive activity by adding recombinant Escherichia coli, which express the active region (InvX:72 a.a.) of Mce1A protein on their external membrane, into cultured HMVEC, using the adhesin involved in the diffuse adherence mechanism. The number of bacteria that invaded into the cells was then measured by a colony counting method. The active region of Mce1A was divided into four sections, and hyperimmune antisera was prepared for each section for analyzing the inhibitory effect against invasion. The invasive activity was suppressed by antibodies against InvX regions 1–24 a.a., 25–46 a.a. and 58–72 a.a. This suggests that the InvX regions 1–24 a.a., 25–46 a.a. and 58–72 a.a. of Mce1A protein play an important role in the invasion of M. leprae into HMVEC and that it may be possible to suppress entry of M. leprae in HMVEC with antibodies against these regions.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Invasion of human microvascular endothelial cells by Mycobacterium leprae through Mce1A protein

Idris

Abdurrahman

Fatulrachman³

et al. 2019

The Journal of Dermatology

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“…Leprosy lesions manifest mainly in cooler body regions, including the skin and mucous membranes of the upper respiratory tract (Yassin et al, 1975; Sato et al, 2007). For nearly a century, investigators attempted to grow M. leprae in various different animals with cool body temperatures (Couret, 1911).…”

Section: Leprosy In Armadillosmentioning

confidence: 99%

The armadillo: a model for neuropathy of leprosy and potentially other neurodegenerative diseases

Sharma

Lahiri

Scollard

et al. 2012

Disease Models &Amp; Mechanisms

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Leprosy (also known as Hansen’s disease) is an infectious peripheral neurological disorder caused by Mycobacterium leprae that even today leaves millions of individuals worldwide with life-long disabilities. The specific mechanisms by which this bacterium induces nerve injury remain largely unknown, mainly owing to ethical and practical limitations in obtaining affected human nerve samples. In addition to humans, nine-banded armadillos (Dasypus novemcinctus) are the only other natural host of M. leprae, and they develop a systemically disseminated disease with extensive neurological involvement. M. leprae is an obligate intracellular parasite that cannot be cultivated in vitro. Because of the heavy burdens of bacilli they harbor, nine-banded armadillos have become the organism of choice for propagating large quantities of M. leprae, and they are now advancing as models of leprosy pathogenesis and nerve damage. Although armadillos are exotic laboratory animals, the recently completed whole genome sequence for this animal is enabling researchers to undertake more sophisticated molecular studies and to develop armadillo-specific reagents. These advances will facilitate the use of armadillos in piloting new therapies and diagnostic regimens, and will provide new insights into the oldest known infectious neurodegenerative disorder.

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“…Leprosy, however, is a disease affecting the skin to produce cutaneous lesions and, despite keratinocytes importance in innate immunity, few studies have mentioned that the epidermis plays a role in leprosy (14)(15)(16)(17)(18). Some evidence indicates a role for dendritic cells (DCs) in immune responses to M. leprae (7), but the other cell type important for epidermal defense, the keratinocyte, is also a source of cytokines and chemokines, which, in turn, are critical in recruiting DCs, T cells and neutrophils into sites of infection (1,19).…”

Section: Introductionmentioning

confidence: 99%

Interaction of Mycobacterium leprae with the HaCaT human keratinocyte cell line: new frontiers in the cellular immunology of leprosy

Lyrio

Campos-Souza

Corrêa

et al. 2015

Experimental Dermatology

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Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae affecting the skin and peripheral nerves. Despite M. leprae invasion of the skin and keratinocytes importance in innate immunity, the interaction of these cells in vitro during M. leprae infection is poorly understood. Conventional and fluorescence optical microscopy, transmission electronic microscopy, flow cytometry and ELISA were used to study the in vitro interaction of M. leprae with the HaCaT human keratinocyte cell line. Keratinocytes uptake of M. leprae is described, and modulation of the surface expression of CD80 and CD209, cathelicidin expression and TNF-α and IL-1β production of human keratinocytes are compared with dendritic cells and macrophages during M. leprae interaction. This study demonstrated that M. leprae interaction with human keratinocytes enhanced expression of cathelicidin and greatly increased TNF-α production. The highest spontaneous expression of cathelicidin was by dendritic cells which are less susceptible to M. leprae infection. In contrast, keratinocytes displayed low spontaneous cathelicidin expression and were more susceptible to M. leprae infection than dendritic cells. The results show, for the first time, an active role for keratinocytes during infection by irradiated whole cells of M. leprae and the effect of vitamin D on this process. They also suggest that therapies which target cathelicidin modulation may provide novel approaches for treatment of leprosy.

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Recombinant Mycobacterium leprae protein associated with entry into mammalian cells of respiratory and skin components

Cited by 15 publications

References 20 publications

Invasion of human microvascular endothelial cells by Mycobacterium leprae through Mce1A protein

Invasion of human microvascular endothelial cells by Mycobacterium leprae through Mce1A protein

The armadillo: a model for neuropathy of leprosy and potentially other neurodegenerative diseases

Interaction of Mycobacterium leprae with the HaCaT human keratinocyte cell line: new frontiers in the cellular immunology of leprosy

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