Recombinant laminin fragments endowed with collagen-binding activity: A tool for conferring laminin-like cell-adhesive activity to collagen matrices

Sato-Nishiuchi, Ryoko; Li, Shaoliang; Ebisu, Fumi; Sekiguchi, Kiyotoshi

doi:10.1016/j.matbio.2017.08.001

Cited by 17 publications

(14 citation statements)

References 47 publications

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“…In particular, collagen IV and perlecan are essential for the mechanical stability of cardiomyocytes and their electrical conduction by binding to the surrounding interstitial ECM and stromal cells [15,16]. In addition, laminin plays an essential role in controlling cell adhesion activity and cell death through cell signaling [17][18][19]. Thus, the ECM, which creates an appropriate microenvironment for myocardial tissue, is crucial for the successful engraftment of transplanted cardiomyocytes.…”

Section: Discussionmentioning

confidence: 99%

Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype

Yokoyama

Miyagawa

Akagi

et al. 2021

Preprint

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The extracellular matrix (ECM) plays a key role in the viability and survival of implanted human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). We hypothesized that coating of three-dimensional (3D) cardiac tissue-derived hiPSC-CMs with the ECM protein fibronectin (FN) would improve the survival of transplanted cells in the heart and improve heart function in a rat model of ischemic heart failure. To test this hypothesis, we first explored the tolerance of FN-coated hiPSC-CMs to hypoxia in an in vitro study. For in vivo assessments, we constructed 3D-hiPSC cardiac tissues (3D-hiPSC-CTs) using a layer-by-layer technique, and then the cells were implanted in the hearts of a myocardial infarction rat model (3D-hiPSC-CTs, n = 10; sham surgery control group (without implant), n = 10). Heart function and histology were analyzed 4 weeks after transplantation. In the in vitro assessment, cell viability and lactate dehydrogenase assays showed that FN-coated hiPSC-CMs had improved tolerance to hypoxia compared with the control cells. In vivo, the left ventricular ejection fraction of hearts implanted with 3D-hiPSC-CT was significantly better than that of the sham control hearts. Histological analysis showed clear expression of collagen type IV and plasma membrane markers such as desmin and dystrophin in vivo after implantation of 3D-hiPSC-CT, which were not detected in 3D-hiPSC-CMs in vitro. Overall, these results indicated that FN-coated 3D-hiPSC-CT could improve distressed heart function in a rat myocardial infarction model with a well-expressed cytoskeletal or basement membrane matrix. Therefore, FN-coated 3D-hiPSC-CT may serve as a promising replacement for heart transplantation and left ventricular assist devices and has the potential to improve survivability and therapeutic efficacy in cases of ischemic heart disease.

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Section: Discussionmentioning

confidence: 99%

Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype

Yokoyama

Miyagawa

Akagi

et al. 2021

Preprint

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“…Human LM221 was produced as a recombinant functionally active E8 fragment containing the collagen-binding domain of fibronectin at the N-terminus to facilitate binding to collagen sheets 23 – 25 . Recombinant LM221 was expressed using a FreeStyle 293 Expression System (Life Technologies, Carlsbad, CA, USA) and purified by sequential affinity chromatography using Ni–NTA agarose (Qiagen, Carlsbad, CA, USA) and anti-FLAG-M2-agarose (Sigma, St. Louis, MO, USA) 16 .…”

Section: Aterials and Methodsmentioning

confidence: 99%

Combined administration of laminin-221 and prostacyclin agonist enhances endogenous cardiac repair in an acute infarct rat heart

Sougawa

Miyagawa

Kawamura

et al. 2021

Sci Rep

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Although endogenous cardiac repair by recruitment of stem cells may serve as a therapeutic approach to healing a damaged heart, how to effectively enhance the migration of stem cells to the damaged heart is unclear. Here, we examined whether the combined administration of prostacyclin agonist (ONO1301), a multiple-cytokine inducer, and stem cell niche laminin-221 (LM221), enhances regeneration through endogenous cardiac repair. We administered ONO1301- and LM221-immersed sheets, LM221-immersed sheets, ONO1301-immersed sheets, and PBS-immersed sheets (control) to an acute infarction rat model. Four weeks later, cardiac function, histology, and cytokine expression were analysed. The combined administration of LM221 and ONO1301 upregulated angiogenic and chemotactic factors in the myocardium after 4 weeks and enhanced the accumulation of ILB4 positive cells, SMA positive cells, and platelet-derived growth factor receptor alpha (PDGFRα) and CD90 double-positive cells, leading to the generation of mature microvascular networks. Interstitial fibrosis reduced and functional recovery was prominent in LM221- and ONO1301-administrated hearts as compared with those in ONO1301-administrated or control hearts. LM221 and ONO1301 combination enhanced recruitment of PDGFRα and CD90 double-positive cells, maturation of vessels, and functional recovery in rat acute myocardial infarction hearts, highlighting a new promising acellular approach for the failed heart.

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“…Alternatively, a recombinant laminin fragment, E8 fragment, which is a truncated protein composed of the C-terminal region of the α, β, and γ chains, was explored. (169) Unlike the recombinant peptide fragments previously described, this truncated protein retains laminin integrin-binding activity, as evidenced by the ability of laminin-511-derived E8 fragment to strongly promote the adhesion and proliferation of human pluripotent stem cells, when compared to Matrigel and the full-length laminin isoform. (170) Accordingly, envisaging the development of collagen matrices with laminin-like adhesive activity, the N-terminal ends of individual chains (α, β, and γ) in the truncated protein were modified with a collagen-binding domain (CBD), to confer collagen-binding activity to the E8 fragment.…”

Section: Hydrogels Functionalized With Laminin-derived Peptidesmentioning

confidence: 99%

“…The developed matrices were shown to support the proliferation of human induced pluripotent stem cells (hiPSCs), in both 2D and 3D, though the morphology of cells grown in each condition was quite different. (169) To better recapitulate the biological activity of laminin chains, in the past few years several studies have been exploring the additive or synergistic effect of the combined incorporation of different laminin cell adhesive peptides (e.g., IKVAV, YIGSR, RGD, AG73, RNIAEIIKDI). For example, a study exploring the modification of fibrin hydrogels with a combination, in equimolar ratios, of RGD, IKVAV, YIGSR, and RNIAEIIKDI showed that these peptides have a synergistic effect on neurite outgrowth when used to bridge a 4 mm gap in a rat dorsal root.…”

Section: Hydrogels Functionalized With Laminin-derived Peptidesmentioning

confidence: 99%

Laminin-Inspired Cell-Instructive Microenvironments for Neural Stem Cells

2019

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Laminin is a heterotrimeric glycoprotein with a key role in the formation and maintenance of the basement membrane architecture and properties, as well as on the modulation of several biological functions, including cell adhesion, migration, differentiation and matrix-mediated signaling. In the central nervous system (CNS), laminin is differentially expressed during development and homeostasis, with an impact on the modulation of cell function and fate. Within neurogenic niches, laminin is one of the most important and well described extracellular matrix (ECM) proteins. Specifically, efforts have been made to understand laminin assembly, domain architecture, and interaction of its different bioactive domains with cell surface receptors, soluble signaling molecules, and ECM proteins, to gain insight into the role of this ECM protein and its receptors on the modulation of neurogenesis, both in homeostasis and during repair. This is also expected to provide a rational basis for the design of biomaterial-based matrices mirroring the biological properties of the basement membrane of neural stem cell niches, for application in neural tissue repair and cell transplantation. This review provides a general overview of laminin structure and domain architecture, as well as the main biological functions mediated by this heterotrimeric glycoprotein. The expression and distribution of laminin in the CNS and, more specifically, its role within adult neural stem cell niches is summarized. Additionally, a detailed overview on the use of full-length laminin and laminin derived peptide/recombinant laminin fragments for the development of hydrogels for mimicking the neurogenic niche microenvironment is given. Finally, the main challenges associated with the development of laminin-inspired hydrogels and the hurdles to overcome for these to progress from bench to bedside are discussed. Version: Postprint (identical content as published paper) This is a self-archived document from i3S -Instituto de Investigação e Inovação em Saúde in the University of Porto Open Repository For Open Access to more of our publications, please visit http://repositorio-aberto.up.pt/ A01/00 Version: Postprint (identical content as published paper) This is a self-archived document from i3S -Instituto de Investigação e Inovação em Saúde in the University of Porto Open Repository For Open Access to more of our publications, please visit http://repositorio-aberto.up.pt/ A01/00 Version: Postprint (identical content as published paper) This is a self-archived document from i3S -Instituto de Investigação e Inovação em Saúde in the University of Porto Open Repository For Open Access to more of our publications, please visit http://repositorio-aberto.up.pt/ A01/00 which is divided into five laminin globular (LG) domains (LG1-LG5) grouped into functional and structural distinct subdomains, LG1-3 and LG4 and LG5.(23) The coiled-coil is thought to help to orientate the LG domains so that they can be available to interact with cells via cell surface receptors, including...

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Recombinant laminin fragments endowed with collagen-binding activity: A tool for conferring laminin-like cell-adhesive activity to collagen matrices

Cited by 17 publications

References 47 publications

Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype

Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype

Combined administration of laminin-221 and prostacyclin agonist enhances endogenous cardiac repair in an acute infarct rat heart

Laminin-Inspired Cell-Instructive Microenvironments for Neural Stem Cells

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