Recombinant Lactobacillus casei Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits

Wang, Li; Xia, Tian; Guo, Tiantian; Ru, Yi; Jin, Yanping; Cui, Wen; Zhou, Han; Qiao, Xin; Tang, Lijie; Xu, Yigang; Li, Yijing

doi:10.3390/vaccines7040172

Cited by 14 publications

(15 citation statements)

References 58 publications

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“…Qiao et al used L. casei as an antigen delivery vehicle to immunize mice and obtained a good immune effect [41]. Wang et al utilized L. casei as an antigen delivery vector to express Rabbit hemorrhagic disease virus (RHDV) capsid protein VP60(VP1)-fused eGFP, and the results indicated that the oral administration of this probiotic vaccine could stimulate SIgA-based mucosal and IgG-based humoral immune responses in rabbits [42]. In this study, we used L. casei ∆Alr W56 to deliver the PEDV S protein-neutralizing epitope COE fragment and the Alr gene and to develop an oral mucosal vaccine against the PEDV and be used as a screen marker in the future.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of Recombinant-Deficient Lactobacillus casei with Complementary Plasmid Expressing Alanine Racemase Gene and Core Neutralizing Epitope Antigen against Porcine Epidemic Diarrhea Virus

Wang

Fan

et al. 2021

Vaccines

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Porcine epidemic diarrhea (PED), which is caused by the porcine epidemic diarrhea virus (PEDV), has occurred worldwide and poses a serious threat to the pig industry. Intestine is the main function site of PEDV; therefore, it is important to develop an oral mucosal immunity vaccine against this virus infection. Most traditional plasmid delivery vectors use antibiotic genes as a selective marker, easily leading to antibiotic accumulation and gene contamination. In this study, to explore whether the alanine racemase gene (Alr) could be used as a screening marker and develop an efficient oral vaccine against PEDV infection, a recombinant strain was constructed using Lactobacillus casei with Alr deletion (L. casei ΔAlr W56) to deliver the Alr gene and a core-neutralizing epitope (COE) antigen. This recombinant bacterium efficiently induced secretory immunoglobulin A (SIgA)-based mucosal and immunoglobulin G (IgG)-based humoral immune responses via oral vaccination in mice. Compared to the other strains, the recombinant bacteria were able to grow without the addition of D-alanine, revealing that Alr in the plasmid could function normally in defective bacteria. This oral mucosal vaccine would provide a useful strategy to substitute the application of antibiotics in the future and induce efficient immune responses against PEDV infection.

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Section: Discussionmentioning

confidence: 99%

Immunogenicity of Recombinant-Deficient Lactobacillus casei with Complementary Plasmid Expressing Alanine Racemase Gene and Core Neutralizing Epitope Antigen against Porcine Epidemic Diarrhea Virus

Wang

Fan

et al. 2021

Vaccines

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“…The gold standard for prevention of the above-mentioned diseases is vaccination; however, there are potential problems associated with its implementation, especially in relation to biosafety. In order to develop new protocols for the control of the rabbit hemorrhagic disease, Wang L. et al (2019) suggested the use of an oral L. casei probiotic vaccine as an antigen delivery system to stimulate humoral immune responses against caliciviruses (Wang L. et al, 2019). However, there are currently no published studies on the use of probiotics to afford FIGURE 2 | Probiotics for control of viral infections.…”

Section: Streptomyces Cinnamoneusmentioning

confidence: 99%

Probiotics at War Against Viruses: What Is Missing From the Picture?

et al. 2020

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Our world is now facing a multitude of novel infectious diseases. Bacterial infections are treated with antibiotics, albeit with increasing difficulty as many of the more common causes of infection have now developed broad spectrum antimicrobial resistance. However, there is now an even greater challenge from both old and new viruses capable of causing respiratory, enteric, and urogenital infections. Reports of viruses resistant to frontline therapeutic drugs are steadily increasing and there is an urgent need to develop novel antiviral agents. Although this all makes sense, it seems rather strange that relatively little attention has been given to the antiviral capabilities of probiotics. Over the years, beneficial strains of lactic acid bacteria (LAB) have been successfully used to treat gastrointestinal, oral, and vaginal infections, and some can also effect a reduction in serum cholesterol levels. Some probiotics prevent gastrointestinal dysbiosis and, by doing so, reduce the risk of developing secondary infections. Other probiotics exhibit anti-tumor and immunomodulating properties, and in some studies, antiviral activities have been reported for probiotic bacteria and/or their metabolites. Unfortunately, the mechanistic basis of the observed beneficial effects of probiotics in countering viral infections is sometimes unclear. Interestingly, in COVID-19 patients, a clear decrease has been observed in cell numbers of Lactobacillus and Bifidobacterium spp., both of which are common sources of intestinal probiotics. The present review, specifically motivated by the need to implement effective new counters to SARS-CoV-2, focusses attention on viruses capable of co-infecting humans and other animals and specifically explores the potential of probiotic bacteria and their metabolites to intervene with the process of virus infection. The goal is to help to provide a more informed background for the planning of future probiotic-based antiviral research.

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“…According to the OIE, specific fluorescence is detected in the liver, spleen and renal glomeruli [ 4 ]. IF has been used mainly for confirming expression of recombinant VP60 protein in infected cells [ 84 , 85 , 86 ]. Other authors used this technique to confirm virus specificity of hybridomas [ 87 , 88 , 89 ].…”

Section: Immunohistochemistrymentioning

confidence: 99%

A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

Abrantes

Lopes

2021

Microorganisms

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Since the early 1980s, the European rabbit (Oryctolagus cuniculus) has been threatened by the rabbit hemorrhagic disease (RHD). The disease is caused by a lagovirus of the family Caliciviridae, the rabbit hemorrhagic disease virus (RHDV). The need for detection, identification and further characterization of RHDV led to the development of several diagnostic tests. Owing to the lack of an appropriate cell culture system for in vitro propagation of the virus, much of the methods involved in these tests contributed to our current knowledge on RHD and RHDV and to the development of vaccines to contain the disease. Here, we provide a comprehensive review of the RHDV diagnostic tests used since the first RHD outbreak and that include molecular, histological and serological techniques, ranging from simpler tests initially used, such as the hemagglutination test, to the more recent and sophisticated high-throughput sequencing, along with an overview of their potential and their limitations.

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Recombinant Lactobacillus casei Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits

Cited by 14 publications

References 58 publications

Immunogenicity of Recombinant-Deficient Lactobacillus casei with Complementary Plasmid Expressing Alanine Racemase Gene and Core Neutralizing Epitope Antigen against Porcine Epidemic Diarrhea Virus

Immunogenicity of Recombinant-Deficient Lactobacillus casei with Complementary Plasmid Expressing Alanine Racemase Gene and Core Neutralizing Epitope Antigen against Porcine Epidemic Diarrhea Virus

Probiotics at War Against Viruses: What Is Missing From the Picture?

A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

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