Recombinant interferon stimulated protein 15 (rISG15) as a molecular marker for detection of early pregnancy in Bubalus bubalis

Batra, Kanisht; Kumar, Aman; Maan, Sushila; Kumar, Vinay; Kumari, Rajni; Nanda, Trilok

doi:10.1016/j.anireprosci.2018.08.018

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…Moreover, early pregnancy diagnosis is essential to identify pregnant females (management) and monitor rates of early embryonic loss. Other types of approaches have already been suggested, such as determining concentrations of pregnancy‐associated glycoproteins (PAGs) (Batra et al., 2018; Thakur et al., 2017), which can be done using machines like those used for progesterone. However, the progesterone has an advantage since PAGs can only be used after 28 days of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Can blood progesterone concentration identify non‐pregnant buffaloes to support oestrous resynchronization?

Codognoto

Souza

Salgado

et al. 2022

Reprod Domestic Animals

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This study compared the plasma progesterone concentrations from pregnant and non‐pregnant buffaloes to identify non‐pregnant females and submit cows earlier to oestrous resynchronization. Forty‐four multiparous mix‐breed Murrah buffaloes were selected for the study. The cows were subjected to hormonal oestrous synchronization and separated into 4 groups, P12 (pregnant, n = 8) and P18 (n = 8) at 12 and 18 days post‐insemination; NP12 (non‐pregnant, n = 7) and NP18 (n = 7) at 23 and 29 days after the onset of synchronization, respectively. The embryos and blood were collected, and the plasma was separated for centrifugation and used to determine progesterone concentration. Progesterone concentration was higher in P18 than P12 (p = .02) and NP18 groups (p < .001). The steroid was also increased in the P12 group compared with NP12 (p = .031). There was no difference between NP12 and NP18 (p = .906). We conclude that the plasma progesterone concentration can be an alternative to identify earlier non‐pregnant buffaloes, advancing the oestrous resynchronization or natural service to improve productivity.

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Section: Discussionmentioning

confidence: 99%

Can blood progesterone concentration identify non‐pregnant buffaloes to support oestrous resynchronization?

Codognoto

Souza

Salgado

et al. 2022

Reprod Domestic Animals

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“…RNA extraction, assessment and cDNA synthesis: Blood samples collected (containing EDTA) were immediately processed for extracting total RNA as per Batra et al (2018). Quality and quantity of RNA was assessed by spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

“…But, the incidence of reproductive challenges like silent heat and short duration heat, reduces the reproductive potential of this species. The phenomenon of silent heat is generally misdiagnosed as pregnancy by farmers (Batra et al 2018), leading to long calving interval and low milk production which is the prime underlying reason for low milk productivity of buffaloes in the Indian subcontinent. This issue can be solved with the use of early pregnancy detection tools in dairy buffaloes, which will allow prompt reinsemination of animals failing to conceive at first service, in turn leading to increased reproductive efficiency and productivity.…”

mentioning

confidence: 99%

Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation

Kumari

Dayal

Sharma

et al. 2021

Indian J of Anim Sci

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The present investigation was aimed to evaluate novel implication of chemokine genes CXCL10 (C-X-C motif chemokine 10) and CCL 8 (C-C motif chemokine 8) genes for use as ideal pregnancy biomarker in dairy buffaloes. We studied expression profile of both these chemokine genes in whole blood of Murrah buffaloes on day 12, 15, 18 and 21 post artificial insemination (AI) using SYBR green chemistry based quantitative real time reverse transcription PCR. Our investigations revealed a consistent increase in transcriptional abundance of CCL8 and CXCL10 genes during this period, although the difference in expression level was not significant between day 15 and 18 post AI for CXCL10 gene. We also studied the effect of parity on the expression profile of these two genes and found that expression level of both these genes is independent of parity status of the animal. Based on the results, it can be concluded that these chemokine genes can be used as early pregnancy biomarker on any day between days 12 to 21 post artificial insemination in buffaloes irrespective of their parity status.

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“…Likewise, novel transcripts such as interleukin 6 ( IFI6), radical S-adenosyl methionine domain containing 2 (RSAD2), interferon induced protein 44 (IFI44), 2′-5′-oligoadenylate synthetase 2 (OAS2 ), galectin 3 binding protein (LGALS3BP), interferon induced transmembrane protein 2 (IFITM2), TNF superfamily member 13B (TNFSF13B), C-type lectin domain family 3 member B (CLEC3B), and dermokine ( DMKN) were expressed in bovine PBMCs during early pregnancy (Rocha et al ., 2020). Other studies showed increased protein expression of myxovirus resistance protein 2 (MX2: Buragohain et al, 2016) and recombinant ISG15 (the product of interferon-stimulated gene 15: Batra et al ., 2018) as well as mRNA expression of ISG15, MX2, 2′-5′-oligoadenylate synthetase 1 ( OAS1 ) and IFNT in PBMC of early pregnant buffalo (Casano et al ., 2022).…”

mentioning

confidence: 99%

Investigation of conceptus stimulated gene expression in buffalo peripheral blood mononuclear cells as potential diagnostic markers of early pregnancy

Sharma,

Choudhary,

Ratta

et al. 2023

Journal of Dairy Research

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Exploration of novel strategies for early pregnancy diagnosis is pivotal in enhancing the reproductive potential and monetary gains from dairy herds. In buffalo, the trophectoderm cells of the elongating conceptus secrete interferon-tau that stimulates the transcription of various genes in peripheral blood mononuclear cells (PBMC) during the peri-implantation period. We explored the differential expression of classical (ISG15) and novel (LGALS3BP and CD9) early pregnancy markers in PBMC of buffaloes during various stages of pregnancy. Natural heat was detected in buffaloes by assessing the vaginal fluid, and artificial insemination (AI) was done. Whole blood was collected from the jugular vein in EDTA-containing vacutainers for PBMC isolation before AI (0-day) and 20, 25 and 40 d post-AI. On day 40, transrectal ultrasonography examination was performed to confirm pregnancy. The inseminated non-pregnant animals served as control. Total RNA was extracted using the TRIzol method. The temporal abundance of ISG15, LGALS3BP and CD9 genes in PBMC was compared between pregnant and non-pregnant groups (n = 9 per group) using real time-qPCR. Results showed transcripts of ISG15 and LGALS3BP were more abundant at 20 d in the pregnant group compared to the 0 d and 20 d values of the non-pregnant group. However, due to variability in expression, threshold (Ct) cycle of RT-qPCR alone could not distinguish pregnant and non-pregnant animals. In conclusion, ISG15 and LGALS3BP transcripts abundance in PBMCs are potential candidate biomarkers for early prediction of buffalo pregnancy 20-days post-AI, but further work is required to allow the development of a reliable new methodology.

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Recombinant interferon stimulated protein 15 (rISG15) as a molecular marker for detection of early pregnancy in Bubalus bubalis

Cited by 6 publications

References 32 publications

Can blood progesterone concentration identify non‐pregnant buffaloes to support oestrous resynchronization?

Can blood progesterone concentration identify non‐pregnant buffaloes to support oestrous resynchronization?

Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation

Investigation of conceptus stimulated gene expression in buffalo peripheral blood mononuclear cells as potential diagnostic markers of early pregnancy

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