RecombinantLactobacillus plantaruminduces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells

Abstract

Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses in mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumor antigen NY-ESO-1. And to investigate the immunostimulatory potency of this new candidate vaccine on human dendritic cells (DCs). L. plantarum displaying NY-ESO-1 induced NY-ESO-1 specific antibodies and T-cell responses in mice. By contrast, L. plantarum displaying conserved proteins such as heat shock protein-27 and galectin-1, did not induce immunity, suggesting that immune tolerance to self-proteins cannot be broken by oral administration of L. plantarum. With respect to immunomodulation, immature DCs incubated with wild type or L. plantarum-NY-ESO-1 upregulated the expression of co-stimulatory molecules and secreted a large amount of interleukin (IL)-12, TNF-a, but not IL-4. Moreover, they upregulated the expression of immunosuppressive factors such as IL-10 and indoleamine 2,3-dioxygenase. Although L. plantarum-matured DCs expressed inhibitory molecules, they stimulated allogeneic T cells invitro. Collectively, the data indicate that L. plantarum-NY-ESO-1 can evoke antigen-specific immunity upon oral administration and induce DC maturation, raising the potential of its use in cancer immunotherapies.

Cloning and Expression of Listeriolysin O in Lactobacillus plantarum

“…26 Furthermore, this strain exhibits natural immuno-adjuanticity and more immunogenicity than L. lactis and L. casei and modulation of immune system toward Th1 responses. 2,5,[28][29][30] On the other hand, LLO facilitates the stimulation of both the CD4+ T cell through MHC class II and CD8+ T cell through MHC class I antigen presentation. 31,32 It is mentioned that nanomer LLO 91-99, is an immunodominant epitope that induces both cell-mediated and humoral immunities.…”

Follistatin‐like protein 1 contributes to dendritic cell and T‐lymphocyte activation in nasopharyngeal carcinoma patients by altering nuclear factor κb and Jun N‐terminal kinase expression

“…This investigation found that DC immunity in NPC patients was augmented by FSTL1. Similarly, TNF‐α has been shown to promote the development of immature DCs into mature DCs . Therefore, the mechanism by which FSTL1 expedites the immune ability of DCs should be elucidated from this perspective.…”

“…Similarly, TNF-α has been shown to promote the development of immature DCs into mature DCs. 54,55 Therefore, the mechanism by which FSTL1 expedites the immune ability of DCs should be elucidated from this perspective. Above all, it is important to investigate the mechanism by which FSTL1 affects DCs to provide a basis for new antitumor immunotherapies.…”

Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens

“…The N-terminal lipoprotein anchor used in this study has previously been used for functional display of invasin from Yersinia pseudotuberculosis (37) and a CCL3 chemokine (39), whereas the C-terminal cell wall anchor has been exploited for functional display of cancer antigens (34,44) and an anti-DEC-205 antibody (40).…”