2019
DOI: 10.1007/s10495-019-01539-7
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Recombinant human lactoferrin induces apoptosis, disruption of F-actin structure and cell cycle arrest with selective cytotoxicity on human triple negative breast cancer cells

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Cited by 51 publications
(41 citation statements)
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“…Cells were cultured in 96-well plates using a number of 1x10 4 cells/well. After cell attachment, they were treated with five different concentrations (20,40,60,80 and 100 nM) of RhodOA solubilized in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA) and rhodamine B aqueous solution for 24, 48 and 72 h. The control cells were represented by the cells treated with DMSO, the solvent of OA derivative conjugated with rhodamine B and water, the solvent used for rhodamine B, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…Cells were cultured in 96-well plates using a number of 1x10 4 cells/well. After cell attachment, they were treated with five different concentrations (20,40,60,80 and 100 nM) of RhodOA solubilized in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA) and rhodamine B aqueous solution for 24, 48 and 72 h. The control cells were represented by the cells treated with DMSO, the solvent of OA derivative conjugated with rhodamine B and water, the solvent used for rhodamine B, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…At the molecular level, hLf was found to induce the apoptotic process by decreasing the anti-apoptotic Bcl-2 and increasing the pro-apoptotic Bax and caspase 3 expressions [154]. Moreover, rhLf produced in the yeast Pichia pastoris has been shown to induce apoptosis through plasma membrane blebbing, cell shrinkage, and chromatin condensation, as well as the disruption of F-actin cytoskeleton organization in the human breast cancer cell line MDA-MB-231 [155]. Moreover, bLf was described to induce apoptosis in stomach cancer cell line SGC-7901 by down-regulating Akt intracellular signaling [156].…”
Section: Lactoferrin Anti-cancer Activity: Induction Of Apoptosismentioning
confidence: 99%
“…Morphological characteristics of the BP and MS MCF7 cells were evaluated with laser-confocal microscopy. Twenty-four hours post-bioprinting, cells were stained as explained elsewhere; (Iglesias-Figueroa et al, 2019) briefly, cells were fixed in 4% formalin for 20 min, washed and incubated in 0.1% v/v Tween 20 in PBS for 10 min at room temperature (RT), washed twice more with permeabilizing solution, incubated again in 200 µl of 5% w/v in bovine serum albumin (BSA; sigma) dissolved in tris-buffered saline solution containing 0.5% v/v Tween 20 for 1 h at RT on a rocker platform. The cells were next stained with the primary antibody Neu (sc-33684, dilution: 1:50) overnight in a rocker platform at 5 • C. The cells were next washed three times with permeabilizing solution and a 1:50 v/v dilution of secondary antibody goat anti-mouse IgG conjugated with Alexa Fluor TM 568 (Invitrogen) was added and then incubated for 1 h on a rocker platform at RT.…”
Section: Stain Processmentioning
confidence: 99%