Recombinant human immunoglobulin (Ig)A1 and IgA2 anti‐D used for detection of IgA deficiency and anti‐IgA

Abstract

The assays for detection of IgA and anti-IgA described in this article are fast and robust. Furthermore, they are applicable in all standard blood typing laboratories and are therefore well suited for immediate investigation of transfusion reactions.

The association of IgA deficiency on infection rate, self‐perceived health, and levels of C‐reactive protein in healthy blood donors

“…At the Department of Clinical Immunology, Copenhagen University Hospital, Copenhagen, an in‐house sandwich ELISA was used for both screening and quantification of IgA deficiency. Microtiter plates were coated with anti‐human IgA, plasma was added, and horseradish peroxidase (HRP)‐conjugated anti‐IgA was used for detection before reading on an ELISA reader, Asys Hitech Expert Plus (Biochrom, Cambridge, UK) . At the Department of Clinical Immunology, Aalborg University Hospital, screening for IgA deficiency was made with an IgA neutralization test.…”

Evaluation of particle gel immunoassays for the detection of severe immunoglobulin A deficiency and anti‐human immunoglobulin A antibodies

“…Identification of patients as IgA deficient with anti‐IgA (Ab) is critical in preventing adverse transfusion reactions since they must either receive blood products from donors known to be IgA deficient or receive products derived from normal, healthy individuals that have been processed in a manner shown to substantially reduce IgA levels 1‐7 . While passive transfusion of Ab to IgA in products to a recipient who is not IgA deficient has not been shown to elicit transfusion reactions, 8‐10 an IgA‐deficient patient's antibody status still remains of concern in the event they receive products containing IgA.…”

Screening of Canadian Blood Services donors for severe immunoglobulin A deficiency

“…Transfusion of blood products that contain immunoglobulin A (IgA) to patients with IgA deficiency who have also developed IgA antibodies can result in serious adverse reactions 1‐7 . In light of reports of the relative scarcity of severely IgA‐deficient whole blood or plasma donors (IgA < 0.05 mg/dL) in North America 1,3,5,8,9 and other countries and the resultant impact on IgA‐deficient component inventories, Canadian Blood Services (CBS) has set up a large‐scale screening program to identify appropriate blood donors.…”