Recombinant human bone morphogenetic protein 2 enhances bone healing in an experimental model of fractures at risk of non-union

Eckardt, Henrik; Christensen, Knud Stenild; Lind, Martin; Hansen, Ebbe Stender; Hall, David W.; Hvid, Ivan

doi:10.1016/j.injury.2004.10.019

Cited by 39 publications

(31 citation statements)

References 13 publications

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“…Second, we did not perform a power analysis or sample size determination at the outset of the study and this is inevitably a protocol flaw. However, the number of subjects is similar to what is used in many similar animal studies using rabbits [19,20,36].…”

Section: Discussionmentioning

confidence: 99%

Thalidomide prolongs survival after experimental musculoskeletal injury, through an effect on mononuclear apoptosis

Panousis¹,

Nikolaou²,

Τσαγανός³

et al. 2014

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 99%

Thalidomide prolongs survival after experimental musculoskeletal injury, through an effect on mononuclear apoptosis

Panousis¹,

Nikolaou²,

Τσαγανός³

et al. 2014

Journal of Surgical Research

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“…Of particular consideration are the many parameters that need to be accurately accounted for: sufficient mixing in the central circulation, good first pass extraction, no separation from the blood, no measurement possible in the second of two capillary beds in series, no obstruction of arterioles, tracer leakage from particle, homogenous particle size, and label intensity. 48 Despite these limitations, it has been used under many different experimental circumstances to measure regional bone blood flow 2,33,37,39,40,43,45, and has been used to validate new blood flow methods in bone such as noble gas washout 34 and laser Doppler flowmetry. 70 The hydrogen washout method of regional blood flow measurement was developed by the Norwegian physiologist Aukland and colleagues 38 and first applied to bone tissue by Whiteside et al 6 in the rabbit; he assumed that his measurements were accurate because they were similar to those reported by other methods.…”

Section: Discussionmentioning

confidence: 99%

“…39 Correct positioning of the tip was confirmed by observation of the ventricular pressure waveform from the pressure transducer. 40 Polystyrene microspheres labeled with 141 Ce, measuring 15.5 AE 0.1 mm in diameter were suspended in 80% saline containing 0.01% Tween 80 (NEN 1 Life Science Products, Boston, MA).…”

Section: Validation Of the Hydrogen Gas Washout Methodsmentioning

confidence: 98%

Measurement of bone blood flow using the hydrogen washout technique—part II: Validation by comparison to microsphere entrapment

Larsen

Pelzer

Friedrich

et al. 2008

Journal Orthopaedic Research

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Accurate and reproducible measurement of bone blood flow has important clinical and experimental applications. Hydrogen washout is simple, safe, and widely used, but its use in bone tissue has not been validated. To this end, we have compared cortical bone blood flow measurements obtained by radioactive-labeled microsphere entrapment with those from hydrogen washout. Blood flow was measured in tibial cortical bone of 12 New Zealand White rabbits by radioactive microsphere entrapment and by hydrogen washout. Besides a control group (n ¼ 6), four animals were treated with systemic epinephrine (0.8 mg/kg/min) (group 2) and two with nitroprusside (100 mg/kg/min) (group 3). Furthermore, nine femora from seven rats were isolated on their vascular pedicles and repeated bone blood flow measurements were made at each location with the hydrogen washout method to confirm reproducibility of blood flow determinations by hydrogen washout. An average flow of 2.3 AE 2.0 mL/min/100 g was obtained with the microsphere method and 2.0 AE 0.5 mL/min/100 g with the hydrogen washout method. There was a significant correlation and agreement: R 2 ¼ 0.97 (p < 0.01). No consistent flow variations were found with systemic vasoactive drug administration. Hydrogen washout provided reproducible results and showed high sensitivity to flow changes. Hydrogen washout is both sensitive and reproducible in measuring bone blood flow. Results agree well with flow values obtained by labeled microsphere entrapment. ß

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“…The present experiment was one arm of a larger experiment and the results from the rhBMP-2 group and from the group that received hyaluronan carrier alone have been published previously [8]. The previous study focused on the effect of rhBMP-2 in the non-union model and compared rhBMP-2 with no treatment.…”

Section: Introductionmentioning

confidence: 99%

Bioabsorbable Barrier Membrane Combined with rhBMP-2 Improved Bone Formation in an Experimental Model of Compromised Healing But Was Not Superior to rhBMP-2 Alone

Eckardt¹,

Christensen²,

Lind³

et al. 2014

OJO

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Objective: Bioabsorbable barrier membranes placed over alveolar ridge bone defects are routinely used in dental surgery to promote bone formation. Combining these osteoconductive membranes with osteoinductive Bone Morphogenetic Proteins could prove useful in long bone fracture treatment. The hypothesis was tested in a clinically relevant model of compromised healing. Methods: Four groups of 8 rabbits underwent unilateral mid-tibial osteotomy, excision of periosteum and endosteum, and plate fixation. One group had rhBMP-2 deposited between the bone ends and Membrane wrapped around the osteotomy, the second group had Membrane wrapped around the osteotomy, the third group had rhBMP-2 placed between the bone ends, and the fourth group received no additional treatment. Results: After 7 weeks, callus size and blood flow were significantly higher in the Membrane+rhBMP-2 group than in the rhBMP-2 treated group, but torsion to failure test showed no significant difference. Membrane treatment and no treatment led to non-union. Conclusion: Absorbable barrier membrane combined with rhBMP-2 enhances bone formation, but has no advantage to rhBMP-2 alone. Membrane alone wrapped around the osteotomy was unable to prevent non-union formation.

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Recombinant human bone morphogenetic protein 2 enhances bone healing in an experimental model of fractures at risk of non-union

Cited by 39 publications

References 13 publications

Thalidomide prolongs survival after experimental musculoskeletal injury, through an effect on mononuclear apoptosis

Thalidomide prolongs survival after experimental musculoskeletal injury, through an effect on mononuclear apoptosis

Measurement of bone blood flow using the hydrogen washout technique—part II: Validation by comparison to microsphere entrapment

Bioabsorbable Barrier Membrane Combined with rhBMP-2 Improved Bone Formation in an Experimental Model of Compromised Healing But Was Not Superior to rhBMP-2 Alone

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