Recombinant Haemagglutinin Derived From the Ciliated Protozoan Tetrahymena thermophila Is Protective Against Influenza Infection

Jawinski, Karina; Hartmann, Marcus W W; Singh, Charanjit; Kinnear, Ekaterina; Busse, David C.; Ciabattini, Annalisa; Fiorino, Fabio; Medaglini, Donata; Trombetta, Claudia Maria; Montomoli, Emanuele; Contreras, Vanessa; Grand, Roger Le; Terrat, Céline; Primard, Charlotte; Verrier, Bernard; Tregoning, John S.

doi:10.3389/fimmu.2019.02661

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…iP-sPAH synthesis was custom-developed by Adjuvatis (Lyon, France) based on i-Particles ® preparation by nanoprecipitation method [52], using poly(d,l-lactic acid) (PLA) as polymer. No surfactant or stabilizer was required to stabilize the colloid solution.…”

Section: Preparation and Characterization Of I-particles Loaded With mentioning

confidence: 99%

Inhibition of Patched Drug Efflux Increases Vemurafenib Effectiveness against Resistant BrafV600E Melanoma

Signetti

Elizarov

Simsir

et al. 2020

Cancers

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Melanoma patients harboring the BRAFV600E mutation are treated with vemurafenib. Almost all of them ultimately acquire resistance, leading to disease progression. Here, we find that a small molecule from a marine sponge, panicein A hydroquinone (PAH), overcomes resistance of BRAFV600E melanoma cells to vemurafenib, leading to tumor elimination in corresponding human xenograft models in mice. We report the synthesis of PAH and demonstrate that this compound inhibits the drug efflux activity of the Hedgehog receptor, Patched. Our SAR study allowed identifying a key pharmacophore responsible for this activity. We showed that Patched is strongly expressed in metastatic samples from a cohort of melanoma patients and is correlated with decreased overall survival. Patched is a multidrug transporter that uses the proton motive force to efflux drugs. This makes its function specific to cancer cells, thereby avoiding toxicity issues that are commonly observed with inhibitors of ABC multidrug transporters. Our data provide strong evidence that PAH is a highly promising lead for the treatment of vemurafenib resistant BRAFV600E melanoma.

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Section: Preparation and Characterization Of I-particles Loaded With mentioning

confidence: 99%

Inhibition of Patched Drug Efflux Increases Vemurafenib Effectiveness against Resistant BrafV600E Melanoma

Signetti

Elizarov

Simsir

et al. 2020

Cancers

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“…There are known techniques utilized to increase the amount of influenza virus in cell cultures during standard processes of producing influenza vaccines (Jawinski et al, 2019). However, influenza vaccines produced using cell cultures have significant limitations in production upscaling.…”

Section: Introductionmentioning

confidence: 99%

Determining optimal conditions for growing recombinant vectors to be used in developing a bovine tuberculosis vaccine

Nurpeisova,

Abay,

Shorayeva

et al. 2023

Research for Rural Development

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Two recombinant influenza A virus vectors expressing the ESAT 6 and TB10.4 mycobacterial proteins from the nonstructural (NS) gene were constructed via reverse genetics technique to develop a specific means of prophylaxis for bovine tuberculosis. We experimented to determine optimal conditions for growing recombinant vectors in Vero cell culture and chick embryos. This study established that the maximum amount of virus builds up in a Vero cell culture with the Dulbecco′s Modified Eagle′s Medium (DMEM) serum-free medium. However, using cell culture to produce vector vaccines is labourintensive and inefficient. An alternative way, a traditional, time-tested technique, is provided by growing samples in chick embryos. One of the advantages of this technique is its affordability and availability, enabling easy scale-up of vaccine production. In the optimization experiments, the FLU-ΔNS_ESAT 6 and FLU-ΔNS_ТВ10.4 viruses constructed were inoculated into 10-day-old chick embryos. It was determined that the optimal incubation temperature that led to the highest virus build-up was 37 ± 0.5 °С. And the infectious activity level of the FLU-ΔNS_ESAT 6 recombinant vector was at 8.95 ± 0.07 log10EID50 0.2 cm-3, while that of the FLU-ΔNS_ТВ10.4 was at 9.20 ± 0.07 log10EID50 0.2 cm-3, what was provided by infectious doses of 1000–10000 EID50, which makes it possible to create a virus-containing material with a hemagglutination activity level of 1:64. The size of recombinant vector amplicons expressing proteins ТВ10.4 and ESAT 6 was 1170 bp and 1175 bp, respectively. Electron microscopy images confirm that the developed virions are morphologically similar to the avian influenza virus.

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Genome-wide identification and in-silico analysis of papain-family cysteine protease encoding genes in Tetrahymena thermophila

DURAN,

ÜSTÜNTANIR DEDE,

DÜNDAR ORHAN

et al. 2024

European Journal of Protistology

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Recombinant Haemagglutinin Derived From the Ciliated Protozoan Tetrahymena thermophila Is Protective Against Influenza Infection

Cited by 3 publications

References 41 publications

Inhibition of Patched Drug Efflux Increases Vemurafenib Effectiveness against Resistant BrafV600E Melanoma

Inhibition of Patched Drug Efflux Increases Vemurafenib Effectiveness against Resistant BrafV600E Melanoma

Determining optimal conditions for growing recombinant vectors to be used in developing a bovine tuberculosis vaccine

Genome-wide identification and in-silico analysis of papain-family cysteine protease encoding genes in Tetrahymena thermophila

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