Recombinant GII.P16 genotype challenges RT-PCR-based typing in region A of norovirus genome

Bonura, Floriana; Urone, Noemi; Bonura, Celestino; Mangiaracina, Leonardo; Filizzolo, Chiara; Sciortino, Giuseppa; Sanfilippo, Giuseppa Luisa; Martella, Vito; Giammanco, Giovanni M.; Grazia, Simona De

doi:10.1016/j.jinf.2021.04.015

Cited by 9 publications

(15 citation statements)

References 27 publications

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“…Of note, most articles assessed in this review pointed out that GII.2 predominance stemmed from the recombinant GII.2[P16] (Lu et al 2021 ; Lun et al 2018 ; Santiso-Bellón et al 2020 ). Reports about AGE cases and outbreaks caused by GII.2[P16] have focused on clinical surveillance in various regions during the winter of 2016 and 2017 (Bidalot et al 2017 , Bonura et al 2021 ; Li et al 2018 ; Medici et al 2018 ). The GII.P16 polymerase also paired with some other capsid genotypes, including the predominant strain GII.4 Sydney 2012.…”

Section: Discussionmentioning

confidence: 99%

Norovirus detection in wastewater and its correlation with human gastroenteritis: a systematic review and meta-analysis

Huang

Zhou

Zhang

et al. 2022

Environ Sci Pollut Res

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Norovirus (NoV) is a major cause of sporadic cases and outbreaks of acute gastroenteritis (AGE), thereby imposing threat to health globally. It is unclear how quantitation of wastewater NoV reflects the incidence of human AGE infections; therefore, we conducted this systematic review and meta-analysis of published NoV wastewater surveillance studies. A literature search was performed, and all studies on NoV wastewater surveillance were identified. Quantitative results were evaluated. The results showed that the overall detection rate of NoV in wastewater was 82.10% (95% confidence interval [CI]: 74.22–89.92%); NoV concentration was statistically significant in terms of season ( P < 0.001), with higher concentration in spring and winter. There were positive correlations between NoV GII concentration in wastewater and GII AGE cases ( r s = 0.51, 95% CI: 0.18–0.74, I 2 = 0%), total AGE cases ( r s = 0.40, 95% CI: 0.15–0.61, I 2 = 23%) and NoV outbreaks ( r s = 0.47, 95% CI: 0.30–0.62, I 2 = 0%). Results of cross-correlation analysis of partial data indicated that variations in GII concentration were consistent with or ahead of those in the number of AGE cases. The diversity of NoV genotypes in wastewater was elucidated, and the dominant strains in wastewater showed a consistent temporal distribution with those responsible for human AGE. Our study demonstrated the potential association of NoV detected in wastewater with AGE infections, and further studies are needed to confirm this conclusion. Supplementary Information The online version contains supplementary material available at 10.1007/s11356-021-18202-x.

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Section: Discussionmentioning

confidence: 99%

Norovirus detection in wastewater and its correlation with human gastroenteritis: a systematic review and meta-analysis

Huang

Zhou

Zhang

et al. 2022

Environ Sci Pollut Res

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“…The norovirus capsid consists of 90 capsid protein dimers, forming a shell of 90 protruding arch-like dimers. 19 Different types of cells in the human gut are involved in norovirus infection. The human gut's predominant cell is a single layer of intestinal epithelial cells (enterocytes), including many immune cells.…”

Section: Discussion Molecular Norovirus In Humansmentioning

confidence: 99%

“…24 NoV, specifically genotypes GII.4, evolves quickly through the mutation and recombination events, resulting in the recurrent emergence of new antigenic variants impacted in the global outbreak of acute gastroenteritis. 16 According to Bonura et al 19 , the majority of norovirus outbreaks and sporadic cases worldwide are caused by norovirus GII.4, with new GII. Phylogenetic analysis revealed that the Taizhou strains GII.P16/GII.2 shared an evolutionary pattern with the strains discovered in the United States and Japan in 2016.…”

Section: Discussion Molecular Norovirus In Humansmentioning

confidence: 99%

“…Indonesia's norovirus genotypes are genetically varied and similar to those seen in Asia and Europe. 19 The norovirus genotypes were dominated by GII, and a small proportion is GI and the combination of GI and GII. The most common strains found in Taizhou, China, are GII.P17/ GII.17, GII.Pe/GII.4, and GII.P16/GII.2.…”

Section: The Study Characteristics Of Molecular Norovirusmentioning

confidence: 99%

“…As shown in Table 1, the population used in the study involved health workers in hospitals in one study 16 , pediatric patients aged under five years with acute gastroenteritis in three studies [17][18][19] , children were suffering from severe gastroenteritis in private and government hospitals in Surabaya, East Java in one study 20 , acute gastroenteritis patients in southeast China in one study. 21 The intervention used in six references is rectal samples detected for norovirus by specific RT-PCR 16 , RT-PCR assay with amplification of region C 17 , RT-PCR assay with norovirus nucleic acid kit using Applied Biosystems 7500 18 , RT-PCR assay with QIAamp Viral RNA kit 19 , Taqman test based on conventional RT-PCR using the Applied Biosystems 7300 system 20 , Multiplex RT-PCR assay with Applied Biosystems 7500 using AgPath-ID One step kit. 21 Two studies stated that norovirus-positive patients had GII norovirus, two studies with GII and GI genotypes, and two studies with GII, GI, combination GII, and GI genotypes as shown in Table 1.…”

Section: The Study Characteristics Of Molecular Norovirusmentioning

confidence: 99%

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Human Norovirus Molecular Analysis and Development of Norovirus Vaccine

Elizafanti

Lusida²,

Miftahussurur³

et al. 2022

IJTID

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The most common organism of acute viral gastroenteritis is norovirus, which accounts for roughly 20% of all occurrences of acute gastroenteritis globally. The virus kills over 200,000 children each year and is the leading cause of childhood diarrhea in the rotavirus-vaccinated population. This study aims to review available studies regarding the information on the genogroup norovirus in humans, development of norovirus vaccines, and eﬀectiveness of norovirus vaccines. A systematic review using Science Direct, PubMed, and Scopus databases to identify eligible case studies. The search was conducted in September-October 2021. The quality of the included literature used checklists from the Critical Appraisal Skills Program (CASP). All of the six selected studies with populations given RT-PCR intervention showed positive for norovirus infection. The most predominant genogroups in humans are GI and GII. As for the research results of the two selected studies on norovirus vaccine, namely the human phase 2 trial containing two Virus-Like Particles (VLP) genotypes, one study showed eﬃcacy at 18-49 one study at ≥ 60 years of age. This study analysis uses Takeda bivalent vaccine. The vaccine includes norovirus antigens of the GI and GII genogroups, intending to expand its protective immune potential. GI, GII, and GIV genogroups are prevalent in humans. VLP that contains GI.I and consensus GII.4c have been created as the NoV vaccine, providing signiﬁ cant eﬃcacy. Very likely because they contain GI dan GII antigens, which are the genogroups that infect humans the most. Patients given a placebo developed acute gastroenteritis due to norovirus GII.2, indicating a genotype cross-reactivity.

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The predominance of recombinant Norovirus GII.4Sydney[P16] strains in children less than 5 years of age with acute gastroenteritis in Tehran, Iran, 2021–2022

Eftekhari,

Kachooei,

Jalilvand

et al. 2023

Virus Research

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Recombinant GII.P16 genotype challenges RT-PCR-based typing in region A of norovirus genome

Cited by 9 publications

References 27 publications

Norovirus detection in wastewater and its correlation with human gastroenteritis: a systematic review and meta-analysis

Norovirus detection in wastewater and its correlation with human gastroenteritis: a systematic review and meta-analysis

Human Norovirus Molecular Analysis and Development of Norovirus Vaccine

The predominance of recombinant Norovirus GII.4Sydney[P16] strains in children less than 5 years of age with acute gastroenteritis in Tehran, Iran, 2021–2022

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