Recombinant expression systems: the obstacle to helminth vaccines?

Geldhof, Peter; Maere, Veerle De; Vercruysse, Jozef; Claerebout, Edwin

doi:10.1016/j.pt.2007.08.012

Cited by 43 publications

(38 citation statements)

References 45 publications

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“…Since the 1990s, more than 80 different recombinant antigens from 22 various helminth species have been characterized (Geldhof et al, 2007). Further, there were high percentages of protection (over 90% reduction in egg counts) observed in taeniid cestodes, such as Taenia ovis, Taenia saginata, and Taenia solium (Lightowlers, 2006).…”

Section: Discussionmentioning

confidence: 99%

Sequence Analysis of theBs-Ag1Gene ofBaylisascaris schroederifrom the Giant Panda and an Evaluation of the Efficacy of a RecombinantBaylisascaris schroederi Bs-Ag1Antigen in Mice

Chen

Wang

et al. 2012

DNA and Cell Biology

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The Baylisascaris schroederi infection rate among wild giant pandas may reach over 50% or even 100%, making it one of the leading causes of death from primary or secondary infection in wild populations. Until now, little was known about how protective immunity to B. schroederi infection could be achieved. The present study was conducted to evaluate the immunogenicity and protective efficacy of recombinant Bs-Ag1 from B. schroederi, by cloning the full-length Bs-Ag1 gene of B. schroederi and expressing it in a heterologous host, Escherichia coli BL21. In mice vaccinated with rBs-Ag1 coupled with Freund's complete adjuvant (FCA), there was a significant reduction (69.2%) in the recovery of challenged B. schroederi L3 compared with either nonvaccinated controls or mice vaccinated with FCA alone. Our study supports the use of Bs-Ag1 as a potential candidate for vaccination against B. schroederi infection and provides basic data for further vaccination trials with mixtures of antigens (with Bs-Ag2 and Bs-Ag3) to B. schroederi.

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Section: Discussionmentioning

confidence: 99%

Sequence Analysis of theBs-Ag1Gene ofBaylisascaris schroederifrom the Giant Panda and an Evaluation of the Efficacy of a RecombinantBaylisascaris schroederi Bs-Ag1Antigen in Mice

Chen

Wang

et al. 2012

DNA and Cell Biology

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“…Over the years, several antigens and antigen mixtures have been evaluated in trials of vaccinations against a range of economically important parasites of cattle and sheep (3)(4)(5)(6). However, the actual mechanisms involved in vaccine-induced immunity to these parasites are still largely unclear.…”

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confidence: 99%

“…To date, only a few candidate vaccines against mucus-dwelling parasites have been developed; one of the most promising is an O. ostertagi vaccine that is based on excretory-secretory (ES) material of the parasite (13,14). Although these experimental vaccines provide the host with significant levels of protection, mimicking this response by using a recombinant version of the antigen, the absolute requirement of the economic viability of a vaccine remains difficult to meet and is currently the main bottleneck in the process of vaccine development (6). Understanding how immunity is achieved in vaccinated animals might help to clarify why the levels of protection achieved by native and recombinant antigens are so different and might result in the optimization of the presently available vaccines.…”

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confidence: 99%

Granule Exocytosis of Granulysin and Granzyme B as a Potential Key Mechanism in Vaccine-Induced Immunity in Cattle against the Nematode Ostertagia ostertagi

et al. 2013

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dOstertagia ostertagi is considered one of the most economically important bovine parasites. As an alternative to anthelmintic treatment, an experimental host-protective vaccine was previously developed on the basis of ASP proteins derived from adult worms. Intramuscular injection of this vaccine, combined with QuilA as an adjuvant, significantly reduced fecal egg counts by 59%. However, the immunological mechanisms triggered by the vaccine are still unclear. Therefore, in this study, the differences in immune responses at the site of infection, i.e., the abomasal mucosa, between ASP-QuilA-vaccinated animals and QuilA-vaccinated control animals were investigated on a transcriptomic level by using a whole-genome bovine microarray combined with histological analysis. Sixty-nine genes were significantly impacted in animals protected by the vaccine, 48 of which were upregulated. A correlation study between the parasitological parameters and gene transcription levels showed that the transcription levels of two of the upregulated genes, those for granulysin (GNLY) and granzyme B (GZMB), were negatively correlated with cumulative fecal egg counts and total worm counts, respectively. Both genes were also positively correlated with each other and with another upregulated gene, that for the IgE receptor subunit (FCER1A). Surprisingly, these three genes were also correlated significantly with CMA1, which encodes a mast cell marker, and with counts of mast cells and cells previously described as globule leukocytes. Furthermore, immunohistochemical data showed that GNLY was present in the granules of globule leukocytes and that it was secreted in mucus. Overall, the results suggest a potential role for granule exocytosis by globule leukocytes, potentially IgE mediated, in vaccine-induced protection against O. ostertagi.

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“…Since the early 1990s, more than 80 different recombinant antigens from 22 different helminth species have been studied (Geldhof et al, 2007). Effective recombinant vaccines have been developed for cestode parasites, including Taenia ovis, T. solium, T. saginata, and Echinococcus granulosus (Lightowlers, 2006).These studies provide important information that could be used for the development of a genetically engineered vaccine against T. pisiformis larvae.…”

Section: Introductionmentioning

confidence: 99%

Protection against Taenia pisiformis larval infection induced by a recombinant oncosphere antigen vaccine

et al. 2014

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ABSTRACT. Taenia pisiformis larvae cause significant health problems to rabbits. At present, it is not known whether the recombinant antigen from the T. pisiformis oncosphere is able to confer protective immunity against T. pisiformis larval infection. The full-length cDNA was cloned into a pET32a (+) vector, and the recombinant protein was then expressed in BL21 (DE3) cells. Vaccination with the purified rTpUbc2 coupled with QuilA was carried out in New Zealand rabbits to evaluate the immunoprotective effect against T. pisiformis infection. The full-length open reading frame of the TpUbc2 gene was 444 bp, and encoded a 16.63-kDa protein. Finally, rTpUbc2 was used to evaluate the ability to induce immunoprotective responses in rabbits. A 79.3-90.8% reduction (P < 0.01) in the recovery of larvae was observed in the experimental group compared to the control group. Specific anti-rTpUbc2 antibodies from immunized rabbits had significantly higher levels of IgG (P < 0.01) compared to the control group; however, no significant difference in IgA levels was found between groups 6149 Protection against Taenia pisiformis larvae ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6148-6159 (2014) (P > 0.05). Our data support the use of rTpUbc2 as a potential candidate to develop a vaccine against T. pisiformis larvae.

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Recombinant expression systems: the obstacle to helminth vaccines?

Cited by 43 publications

References 45 publications

Sequence Analysis of theBs-Ag1Gene ofBaylisascaris schroederifrom the Giant Panda and an Evaluation of the Efficacy of a RecombinantBaylisascaris schroederi Bs-Ag1Antigen in Mice

Sequence Analysis of theBs-Ag1Gene ofBaylisascaris schroederifrom the Giant Panda and an Evaluation of the Efficacy of a RecombinantBaylisascaris schroederi Bs-Ag1Antigen in Mice

Granule Exocytosis of Granulysin and Granzyme B as a Potential Key Mechanism in Vaccine-Induced Immunity in Cattle against the Nematode Ostertagia ostertagi

Protection against Taenia pisiformis larval infection induced by a recombinant oncosphere antigen vaccine

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