Recombinant expression and characterization of Lucilia cuprina CYP6G3: Activity and binding properties toward multiple pesticides

Traylor, Matthew J.; Baek, Jong‐Min; Richards, Katelyn E.; Fusetto, Roberto; Huang, Weiliang; Josh, Peter; Chen, Zhenzhong; Bollapragada, Padma; O’Hair, Richard A. J.; Batterham, Philip; Gillam, Elizabeth M. J.

doi:10.1016/j.ibmb.2017.09.004

Cited by 12 publications

(4 citation statements)

References 64 publications

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“…Obviously, CYP6G4 was significantly overexpressed in the propoxur‐resistant strain compared to the susceptible strain in the two genders, being most probably related to propoxur metabolism. CYP6G enzymes have been proved to confer metabolism of insecticides in several species, 15–18 . Moreover, many studies about regulatory mutations (cis or trans) on changes of P450s expression have been reported 43 .…”

Section: Discussionmentioning

confidence: 99%

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The overexpression and variant of CYP6G4 associated with propoxur resistance in the housefly, Musca domestica L.

You

Shan

et al. 2021

Pest Management Science

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BACKGROUND The control of the housefly, Musca domestica, heavily relies on the application of insecticides. Propoxur, a carbamate, has been widely used for vector control. The housefly populations with high propoxur resistance display point mutations and overexpression of acetylcholinesterase. However, the roles of cytochrome P450 monoxygenases (P450s), as important detoxification enzymes, remain poorly understand in the housefly resistant to propoxur. RESULTS P450s were implied to contribute to propoxur resistance based on the synergism of piperonyl butoxide (PBO) and the increase of P450 enzyme activity in the near‐isogenic line propoxur resistant strain (N‐PRS). Five P450 genes (CYP6G4, CYP6A25, CYP304A1, CYP6D3, and CYP6A1) by RNA‐sequencing comparison were significantly up‐regulated in the N‐PRS strain with >1035‐fold resistance to propoxur. A total of 13 non‐synonymous mutations of three P450 genes (CYP6G4, CYP6D3, and CYP6D8) were found in the N‐PRS strain. The amino acid substitutions of CYP6D3 and CYP6D8 were probably not resistance‐associated single nucleotide polymorphisms (SNPs) because they were also found in the aabys susceptible strain. However, CYP6G4 variant in the N‐PRS strain was not found in the aabys strain. The conjoint analysis of mutations and a series of genetic crosses exhibited that the housefly propoxur resistance was strongly associated with the mutations of CYP6G4 gene. CONCLUSION Our results suggested that a combination of up‐regulated transcript levels and mutations of CYP6G4 contributed to propoxur resistance in the housefly. © 2021 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

“…CYP6G2 was reported to be linked to nitenpyram and diazinon resistance in D. melanogaster 17 . In Lucilia cuprina , the recombinant CYP6G3 has been characterized for its ability to metabolize diazinon and imidacloprid 18 . CYP6M2 and CYP6P3 were identified to be involved in permethrin resistance in An .…”

Section: Introductionmentioning

confidence: 99%

The overexpression and variant of CYP6G4 associated with propoxur resistance in the housefly, Musca domestica L.

You

Shan

et al. 2021

Pest Management Science

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“…Although many studies have shown a correlation between CYP6 overexpression and resistance, few studies have functionally validated the ability of these enzymes to detoxify an insecticide, as shown by the analysis of the available data in the genus Spodoptera [64]. Few examples also demonstrate the role of CYP6 in the resistance to OPs: in Helicoverpa zea, diazinon is metabolized by CYP6B8 [65] while it is the CYP6G3 that does so in Lucilia cuprina [66]. The role of CYP6NW1 should also be investigated, as it is the most over-expressed in earwigs from ORG and IPM, although there is almost no treatment in common between these two types of orchards (Table 2).…”

Section: The Expression Of Detoxification Enzymesmentioning

confidence: 99%

The Molecular Resistance Mechanisms of European Earwigs from Apple Orchards Subjected to Different Management Strategies

Fricaux,

Le Navenant,

Siegwart

et al. 2023

Insects

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To date, apple orchards are among the most treated crops in Europe with up to 35 chemical treatments per year. Combining control methods that reduce the number of pesticide treatments is essential for agriculture and more respectful of the environment, and the use of predatory insects such as earwigs may be valuable to achieve this goal. European earwigs, Forficula auricularia (Dermaptera: Forficulidae) are considered beneficial insects in apple orchards where they can feed on many pests like aphids. The aim of this study was to investigate the potential impact of orchards’ insecticide treatments on resistance-associated molecular processes in natural populations of earwigs. Because very few molecular data are presently available on earwigs, our first goal was to identify earwig resistance-associated genes and potential mutations. Using earwigs from organic, integrated pest management or conventional orchards, we identified mutations in acetylcholinesterase 2, α1 and β2 nicotinic acetylcholine receptors. In addition, the expression level of these targets and of some essential detoxification genes were monitored using RT-qPCR. Unexpectedly, earwigs collected in organic orchards showed the highest expression for acetylcholinesterase 2. Four cytochromes P450, one esterase and one glutathione S-transferases were over-expressed in earwigs exposed to various management strategies in orchards. This first study on resistance-associated genes in Forficula auricularia paves the way for future experimental studies aimed at better understanding the potential competition between natural enemies in apple orchards in order to optimize the efficiency of biocontrol.

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“…Cytochrome P450 reductase (CPR) is an essential electron transfer protein located on the endoplasmic reticulum of most cell types that plays an important role in cytochrome P450-mediated drug and insecticide metabolism ( Yamazaki et al, 2002 ; Kashiwada et al, 2005 ; Murataliev et al, 2008 ; Traylor et al, 2017 ). Because CPR can act as obligatory electron donors for P450s, it may also be linked to the development of insecticide resistance in insects ( Liu and Scott, 1996 ; Zhang and Scott, 1996 ).…”

Section: Introductionmentioning

confidence: 99%

The Central Role of Multiple P450 Genes and Their Co-factor CPR in the Development of Permethrin Resistance in the Mosquito Culex quinquefasciatus

Gong

et al. 2022

Front. Physiol.

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Mosquitoes’ increasing resistance to insecticides is becoming a major threat for control efforts worldwide. Multiple P450 genes that are up-regulated in permethrin resistant strains of Culex quinquefasciatus have been linked to the development of resistance. In the current study, we characterized the function of six P450 genes, CYP6P14, CYP6BZ2, CYP9J33, CYP9J34, CYP9J40, and CYP9J45, that are overexpressed in the permethrin resistant Culex mosquitoes and showed their capability in metabolism of permethrin. These six P450 genes can convert 3-phenoxybenzoic alcohol (PBCHO) to a less toxic product, 3-phenoxybenzoic acid (PBCOOH), indicating that these P450s play an important role in permethrin degradation pathways. Although we know multiple P450 genes are over-expressed in permethrin resistant Culex mosquitoes, it remains to be seen whether cytochrome P450-reductase (CPR) gene that are co-overexpressed with P450 genes in permethrin resistant mosquitoes do indeed serve as a resistance mechanism. An in-depth investigation of the expression of CPR gene in resistant mosquitoes was conducted in permethrin resistant mosquitoes. The finding of CPR gene overexpression in permethrin resistant mosquitoes suggested the importance of co-overexpression of multiple P450 genes with their obligatory electron donor CPR in the complex detoxification system, boosting the metabolism of permethrin and hence the development of permethrin resistance in Cx. quinquefasciatus.

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Recombinant expression and characterization of Lucilia cuprina CYP6G3: Activity and binding properties toward multiple pesticides

Cited by 12 publications

References 64 publications

The overexpression and variant of CYP6G4 associated with propoxur resistance in the housefly, Musca domestica L.

The overexpression and variant of CYP6G4 associated with propoxur resistance in the housefly, Musca domestica L.

The Molecular Resistance Mechanisms of European Earwigs from Apple Orchards Subjected to Different Management Strategies

The Central Role of Multiple P450 Genes and Their Co-factor CPR in the Development of Permethrin Resistance in the Mosquito Culex quinquefasciatus

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