Recombinant expression and anti-microbial activity of anti-lipopolysaccharide factor (ALF) from the black tiger shrimp

Somboonwiwat, Kunlaya; Marcos, Michael; Tassanakajon, Anchalee; Klinbunga, Sirawut; Aumelas, André; Romestand, Bernard; Gueguen, Yannick; Boze, Hélène; Moulin, Gabrielle; Bachère, Evelyne

doi:10.1016/j.dci.2005.02.004

Cited by 190 publications

(124 citation statements)

References 36 publications

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“…In the present study, the mature peptide of EsALF was expressed in E. coli BL21(DE3)-pLysS, and rEsALF exhibited broad-spectrum bactericidal activity towards Gram-positive and Gram-negative bacteria, which was almost consistent with recombinant ALF from T. tridentatus [20,32] and P. monodon [22]. However, the lytic activity of rEsALF against Gram-negative bacteria was relatively weak compared with recombinant ALF in horseshoe crab and shrimp, especially toward E. coli Top10F'.…”

Section: Discussionsupporting

confidence: 54%

“…It was firstly found as a potent anticoagulant from Limulus polyphemus and Tachypleus tridentatus amebocytes, which inhibited the endotoxin-mediated activation of the coagulation cascade [19]. Subsequent studies indicated that ALF from L. polyphemus exhibited strong antibacterial activity against Gram-negative R-type bacteria through neutralizing the lipid A, a common toxic moiety of LPS [21,22], and also the inhibitory effect on the growth of Gram-positive bacteria. Afterward, similar proteins were identified from the haemocytes of Litopenaeus vannamei [15], T. tridentatus [20], Penaeus monodon [17,18], Fenneropenaeus chinensis [9], Marsupenaeus japonicus [16], Pacifastacus leniusculus [23], and Scylla paramamosain [24], etc.…”

Section: Introductionmentioning

confidence: 99%

“…The expression profile of ALFs indicated that the mRNA transcripts for the protein could be induced by Vibrio infection [9,18] or LPS administration [16], suggesting their crucial roles in crustacean innate immunity. More importantly, the discovery of various ALFs and their derivatives had attracted great interest in mammalian health as candidate therapeutic or prophylactic agents for the management of septic shocks [22,25,26].…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis

Zhao

Song

et al. 2008

Developmental & Comparative Immunology

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis

Zhao

Song

et al. 2008

Developmental & Comparative Immunology

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“…Three kinds of AMP have been fully characterized in penaeid shrimps; the penaeidins and the anti-lipopolysaccharide factor (ALF) from haemocytes [1,26] and anionic haemocyanin derived peptides from plasma [27]. Penaeidins are the diverse family of cationic peptides [28] and are classified into four classes (PEN-2, PEN-3, PEN-4 and PEN-5) [7,8,10,12,13,15e 20,22,24,25].…”

Section: Introductionmentioning

confidence: 99%

Adaptive evolution after duplication of penaeidin antimicrobial peptides

Padhi

Verghese

Otta

et al. 2007

Fish & Shellfish Immunology

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Penaeidin antimicrobial peptides in penaeid shrimps are an important component of their innate immune system that provides immunity against infection caused by several gram-positive bacteria and filamentous fungal species. Despite the knowledge on the identification and characterization of these peptides in penaeid shrimps, little is known about the evolutionary pattern of these peptides and the underlying genetic mechanisms that maintain high sequence diversities in the penaeidin gene family. Based on the phylogenetic analyses and maximum likelihood-based codon substitution analyses, here we present the convincing evidence that multiple copies of penaeidins have evolved by gene duplication, and positive Darwinian selection (adaptive evolution) is the likely cause of accelerated rate of amino acid substitutions among these duplicated genes.While the average ratio of non-synonymous to synonymous substitutions (u) for the entire coding region of both active domains is 0.9805, few codon sites showed significantly higher u (3.73). The likelihood ratio tests that compare models incorporating positive selection (u > 1) at certain codon sites with models not incorporating positive selection (u < 1), failed to reject (p ¼ 0) the evidence of positive Darwinian selection. The rapid adaptive evolution of this gene family might be directed by the pathogens and the faster rate of amino acid substitutions in the N-terminal proline-rich and C-terminal cysteine-rich domains could be due to their direct involvement in the protection against pathogens. When the host expose to different habitats/environment an accelerated rate of amino acid substitutions in both the active domains may also be expected.

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“…The mRNAs for lysozyme, a homolog of tachylectin (from horseshoe crab), and antilipopolysaccharide factor were significantly upregulated in infected hepatopancreas. The antimicrobial activity of these proteins (against bacteria and fungi) has been well established (12,24,29,50), but their potential involvement in antiviral responses has remained largely unexplored. Overall, these results suggest that infection with WSSV activates responses that overlap (at least partially) with those induced by challenge with bacteria (51,52).…”

Section: Changes In Gene Expression In Response To Immune Challengementioning

confidence: 99%

Insights into the immune transcriptome of the shrimpLitopenaeus vannamei: tissue-specific expression profiles and transcriptomic responses to immune challenge

Robalino

Almeida

McKillen

et al. 2007

Physiological Genomics

122

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Infectious disease constitutes a major obstacle to the sustainability of shrimp aquaculture worldwide and a significant threat to natural populations of shrimp and other crustacea. The study of the shrimp immune system, including the response to viral infection, has been hampered by a relative lack of molecular genetic information and of tools suitable for high-throughput assessment of gene expression. In this report, the generation of a cDNA microarray encompassing 2,469 putative unigenes expressed in gills, circulating hemocytes, and hepatopancreas of Litopenaeus vannamei is described. The unigenes printed on the microarray were derived from the analyses of 7,021 expressed sequence tags obtained from standard cDNA libraries as well as from libraries generated by suppression subtractive hybridization, after challenging shrimp with a variety of immune stimuli. The general utility of the cDNA microarray was demonstrated by interrogating the array with labeled RNA from four different shrimp tissues (gills, hemocytes, hepatopancreas, and muscle) and by analyzing the transcriptomic response of shrimp to a lethal challenge with white spot syndrome virus. Our results indicate that white spot syndrome virus infection upregulates (in the hepatopancreas) genes encoding known and potential antimicrobial effectors, while some genes involved in protection from oxidative stress were found to be downregulated by the virus.

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Recombinant expression and anti-microbial activity of anti-lipopolysaccharide factor (ALF) from the black tiger shrimp

Cited by 190 publications

References 36 publications

Molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis

Molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis

Adaptive evolution after duplication of penaeidin antimicrobial peptides

Insights into the immune transcriptome of the shrimpLitopenaeus vannamei: tissue-specific expression profiles and transcriptomic responses to immune challenge

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