Recombinant Escherichia coli for the biomonitoring of benzene and its derivatives in the air

Berno, Elisa; Marcondes, D.F. Pereira; Gamalero, S. Ricci; Eandi, Mario

doi:10.1016/j.ecoenv.2003.10.005

Cited by 16 publications

(4 citation statements)

References 7 publications

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“…The reproducibility and simplicity of this biosensor makes it a reliable initial warning device for indoor air monitoring. Another whole cell application is the use of E-coli which contains the TOL plasmid (responsible for the degradation of benzene and its derivatives) fused with the genes of firefly luciferase (118). This application offers a rapid, inexpensive and sensitive technique for environmental detection of aromatic pollutant compounds.…”

Section: Conventional and Biosensor Techniques For Indoor Pollutant Dmentioning

confidence: 99%

Indoor air pollution and the contribution of biosensors

Eltzov

Cesarea²,

Low

et al. 2019

The EuroBiotech Journal

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A vast majority of people today spend more time indoors than outdoors. However, the air quality indoors may be as bad as or even worse than the air quality outside. This is due to the continuous circulation of the same air without proper ventilation and filtration systems, causing a buildup of pollutants. As such, indoor air quality monitoring should be considered more seriously. Indoor air quality (IAQ) is a measure of the air quality within and around buildings and relates to the health and comfort of building occupants. To determine the IAQ, computer modeling is done to simulate the air flow and human exposure to the pollutant. Currently, very few instruments are available to measure the indoor air pollution index. In this paper, we will review the list of techniques available for measuring IAQ, but our emphasis will be on indoor air toxicity monitoring.

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Section: Conventional and Biosensor Techniques For Indoor Pollutant Dmentioning

confidence: 99%

Indoor air pollution and the contribution of biosensors

Eltzov

Cesarea²,

Low

et al. 2019

The EuroBiotech Journal

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“…This portable biosensor kit showed remarkable potential for practical application; it was achieved by increasing the retention period. Also, a portable microbial biosensor was reported by Berno et al ( 2004 ), which detects benzene not only in laboratory samples but also in outdoor samples for in situ testing. E. coli HB101 harboring pTSN316 cultured on solid-soil dish could be used in outdoor due to portable colorimeter integrating with batteries and alimentation support.…”

Section: Micro/nanotechnological Platforms For Microbial Biosensorsmentioning

confidence: 99%

Review of Micro/Nanotechnologies for Microbial Biosensors

Lim

Lee

et al. 2015

Front. Bioeng. Biotechnol.

110

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A microbial biosensor is an analytical device with a biologically integrated transducer that generates a measurable signal indicating the analyte concentration. This method is ideally suited for the analysis of extracellular chemicals and the environment, and for metabolic sensory regulation. Although microbial biosensors show promise for application in various detection fields, some limitations still remain such as poor selectivity, low sensitivity, and impractical portability. To overcome such limitations, microbial biosensors have been integrated with many recently developed micro/nanotechnologies and applied to a wide range of detection purposes. This review article discusses micro/nanotechnologies that have been integrated with microbial biosensors and summarizes recent advances and the applications achieved through such novel integration. Future perspectives on the combination of micro/nanotechnologies and microbial biosensors will be discussed, and the necessary developments and improvements will be strategically deliberated.

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“…Some researchers have proposed a bioassay using immobilized luminous bacteria (Schreiter et al, 2001;Premkumar et al, 2001;Christo et al, 2002;Lee et al, 2003;Park et al, 2005;Sakaguchi et al, 2007;Tamia, 2007;Chu et al, 2009 (Lee et al, 2003;Tani et al, 2004;Yoo et al, 2007;Yu et al, 2008). (Gu, Choi, 2001;Rastorguev, Zavil'gelsky, 2001;Abd-El-Haleem et al, 2002, 2006Bechor et al, 2002;Ren, Frymier, 2003Berno et al, 2004;Ivask et al, 2004;Lovanh, Alvarez, 2004;Lang et al, 2005;Toba, Hay, 2005;Wiles et al, 2005;Lee et al, 2006Lee et al, , 2007aLeedjarv et al, 2006;Grande et al, 2007;Chinalia et al, 2008). The possibility of using lux genes as markers of gene expression is important for studying pathogeny, virulence, adaptations and secondary metabolism (Van Dyk, 1998;Lovanh, Alvarez, 2004;Qazi et al, 2004).…”

Section: Preparing the Test Object 211 Alive Test Bacterial Culturementioning

confidence: 99%

Bioluminescent Bioassays Based on Luminous Bacteria

K.¹,

M.²,

A.³

et al. 2009

J. Sib. Fed. Univ., Biol.

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Bioluminescent analysis is one of the most promising express methods for biologically monitoring the environment because the luminescent system is highly sensitive to even micro quantities of pollutants. Bioassays based on luminous bacteria give an integral estimation of toxicity and frequently surpass other known bioassays in speed, accuracy, sensitivity and simplicity. The enzymes of bacterial luminescent system are also used in developing highly sensitive analytical methods for practical purposes. This paper considers the main features of bacterial bioluminescence and isolated luminescent system, and also the applications of bioluminescence. One part reviews the investigations on the influence of different chemical substances on bacterial luminescence, development and using of bioluminescent bioassays, in particular some of them, in environmental monitoring of several regions in Russia. Luminous bacteria strains from the Culture Collection of Institute of Biophysics SB RAS (CCIBSO 836) are successfully used to produce new or improved bioassays based on lyophilized bacteria bearing the lux gene. Such bioassays are suitable for biotesting of water, air, soil, and the chemical substances used in everyday life. The «know-how» of kits for bioluminescent analysis and bioassays based on lyophilized natural luminous bacteria P.phosphoreum and recombinant Е.соli strain with cloned lux genes is developed. The bioluminescent assay has certificate and is recommended as an additional method of ecological monitoring in parallel with the other bioassays. The sensitivity of the bioassays developed in the IBP SB RAS is comparable to that of the foreign analogues Microtox ©TM , ToxAlert ©TM , etc.

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Recombinant Escherichia coli for the biomonitoring of benzene and its derivatives in the air

Cited by 16 publications

References 7 publications

Indoor air pollution and the contribution of biosensors

Indoor air pollution and the contribution of biosensors

Review of Micro/Nanotechnologies for Microbial Biosensors

Bioluminescent Bioassays Based on Luminous Bacteria

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