Recombinant Erns-E2 protein vaccine formulated with MF59 and CPG-ODN promotes T cell immunity against bovine viral diarrhea virus infection

Wang, Shenghua; Yang, Guanghui; Nie, Jiawei; Yang, Ren-Jie; Du, Mengze; Su, Jin-Hui; Wang, Jing; Zhu, Yaohong

doi:10.1016/j.vaccine.2020.03.020

Cited by 16 publications

(10 citation statements)

References 47 publications

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“…Peripheral blood mononuclear cells (PBMCs) isolated from the blood plasma of mice at 21 d were purified and collected as previously described [ 9 ]. Cells were stained with following monoclonal antibodies: fluorescein isothiocyanate–conjugated anti-mouse CD3e (clone 145-2C11, 11-0311-82, Thermo Fisher Scientific, Waltham, MA, USA), Percp-Cyanine5.5-conjugated anti-mouse CD8 (clone 53- 6.7, 5-0081-80, Thermo Fisher Scientific, Waltham, MA, USA), Apc-Cy7-conjugated anti-mouse CD4 (clone GK15, 561830, BD Biosciences, San Jose, CA, USA), APC-conjugated anti-mouse IL-2 (clone JES6-5H4, 17-7021-81, Thermo Fisher Scientific, Waltham, MA, USA), and PE-Cyanine7-conjugated anti-mouse TNF-α (clone MP6-XT22, 25-7321-82, Thermo Fisher Scientific) in the dark for 30 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Meanwhile, for examining to BVDV viral antigen in spleen tissue samples, sections of pathological tissue were performed with immunohistochemistry. The experimental program and scored were as previously described [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

“…Effective subunit vaccines which address these challenges are in high demand [ 8 ]. However, subunit vaccines based on soluble, monomeric proteins frequently suffer from a limited immunogenicity and require potent adjuvants, high antigen doses, and repetitive vaccinations [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection

Yang

Wang

et al. 2022

IJMS

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Bovine viral diarrhea virus (BVDV) is a critical animal pathogen that leads to cattle production losses associated with acute disease, immune dysregulation, reproductive failure, and respiratory disease. Due to the monotonous control technique and neglect of BVDV, increasing prevalence of BVDV has caused significant economic losses in the cattle industry worldwide. Therefore, novel anti-BVDV drugs are essential to prevent and control BVDV. Our previous studies have found that Forsythoside A (FTA) could inhibit the replication of BVDV via TRAF2-dependent CD28-4-1BB signaling in bovine peripheral blood mononuclear cells (PBMCs), but whether they can directly inhibit the BVDV remains unclear. Here, we further investigated the effects of FTA on BVDV and its underlying mechanisms of action. We found that FTA significantly inhibited the replication of BVDV in the MDBK cell directly. The results demonstrated that FTA could reduce the functional activation of Caspase-1 to inhibit the inflammatory response caused by BVDV infection and increase the expression of type I interferon (IFN-I) to clear the virus in vitro. The animal experiment was performed to evaluate the antiviral effect of FTA in vivo. Notably, after challenged with BVDV, mice with FTA + Erns-E2 protein displayed alleviated pathological damage and decreased the viral load in the spleen compared with mice inoculated with Erns-E2 protein. Furthermore, treatment with FTA enhanced body defense and delayed infection by the BVDV. Our results reveal that FTA suppresses BVDV replication both in vitro and in vivo and therefore shows promise as an anti-BVDV agent.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection

Yang

Wang

et al. 2022

IJMS

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“…One such approach, DNA vaccination, has been trialled with mixed success and is generally deemed to be effective but comes with challenges such as the optimal route of delivery. The use of recombinant subunit proteins had earlier been deemed insufficient to provide effective protection, not least when focusing on E2 [ 39 , 78 , 79 , 80 , 81 ]. The combination of antigens (e.g., E2 and NS3) in formulations with molecular adjuvants to stimulate antigen-presenting cells [ 58 , 59 ] has been more successful, but requires further refinement.…”

Section: Vaccination Against Bovine Pestivirusesmentioning

confidence: 99%

Bovine Pestivirus Heterogeneity and Its Potential Impact on Vaccination and Diagnosis

Riitho

Strong

Larska

et al. 2020

Viruses

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Bovine Pestiviruses A and B, formerly known as bovine viral diarrhoea viruses (BVDV)-1 and 2, respectively, are important pathogens of cattle worldwide, responsible for significant economic losses. Bovine viral diarrhoea control programmes are in effect in several high-income countries but less so in low- and middle-income countries where bovine pestiviruses are not considered in disease control programmes. However, bovine pestiviruses are genetically and antigenically diverse, which affects the efficiency of the control programmes. The emergence of atypical ruminant pestiviruses (Pestivirus H or BVDV-3) from various parts of the world and the detection of Pestivirus D (border disease virus) in cattle highlights the challenge that pestiviruses continue to pose to control measures including the development of vaccines with improved cross-protective potential and enhanced diagnostics. This review examines the effect of bovine pestivirus diversity and emergence of atypical pestiviruses in disease control by vaccination and diagnosis.

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“…Its wide distribution and transmission between various hosts increase the difficulty in controlling its spread. Upon infection on a host, BVDV invades and damages multiple organs and tissues including the brain, kidney, lung, liver, intestine, and spleen by altering the major component of the cells such as the tight junction proteins (TJs) [ 6 , 7 , 8 ]. Tight junction proteins such as occludin and claudin-1 maintain epithelial barrier functions, thus, their alterations increase the paracellular permeability [ 9 ] where BVDV uses them as co-receptors for cell entry [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Melatonin as Immune Potentiator for Enhancing Subunit Vaccine Efficacy against Bovine Viral Diarrhea Virus

et al. 2021

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Bovine viral diarrhea virus (BVDV) is a pathogen associated with substantial economic losses in the dairy cattle industry. Currently, there are no effective vaccines against BVDV. Melatonin (MT) has been shown to have anti-inflammatory and anti-viral properties, and the use of MF59 in vaccines significantly enhances vaccine efficiency. Here, MT and MF59 were added into the Erns-LTB vaccine. Subsequently, their inhibitory activity on the NF-κB signaling pathway in Mardin-Darby Bovine Kidney cells and the hippocampus was assessed using western blot and quantitative reverse transcription PCR. The findings revealed that MT in the Erns-LTB vaccine decreases the phosphorylation of p65 proteins caused by BVDV infection. In addition, MT decreased the mRNA levels of IL-1β and IL-6 in vitro, but increased the production of IFN-α, IFN-β, Mx1 in vitro, brain-derived neurotrophic factor, cyclic amp response element-binding protein, and the stem cell factor in vivo. Furthermore, treatment with Erns-LTB + MF59 + MT stimulated the production of T lymphocytes, alleviated pathological damage, decreased expressions of BVDV antigen, and tight junction proteins in mice. These findings imply that MT has potential for use in the Erns-LTB vaccine to inhibit BVDV infection and regulate the immune responses of T-cells by inhibiting the NF-κB signaling pathway.

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Recombinant Erns-E2 protein vaccine formulated with MF59 and CPG-ODN promotes T cell immunity against bovine viral diarrhea virus infection

Cited by 16 publications

References 47 publications

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection

Bovine Pestivirus Heterogeneity and Its Potential Impact on Vaccination and Diagnosis

Melatonin as Immune Potentiator for Enhancing Subunit Vaccine Efficacy against Bovine Viral Diarrhea Virus

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