Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection

Rocha, Eliseu Soares de Oliveira; Oliveira, Jaquelline Germano de; Santos, João Rodrigues dos; Rodrigues, Gisele Olinto Libânio; Figueiredo, Leandra Barcelos; Pessanha, José Eduardo Marques; Proietti, Fernando Augusto; Fonseca, Flávio Guimarães da; Bonjardim, Cláudio Antônio; Ferreira, Paulo César Peregrino; Kroon, Erna Geessien

doi:10.1016/j.jviromet.2012.09.012

Cited by 15 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it may yield false-positive results (e.g., 1-2% of the test samples), resulting in an erroneous interpretation of seroepidemiology. Therefore, a neutralization assay is required to confirm the presence of virusspecific antibodies in serum samples (22)(23)(24). Among the 1,646 sera examined, 18 (1.1%) tested positive in the IgG ELISA.…”

Section: Discussionmentioning

confidence: 99%

Low Seroprevalence of Severe Fever with Thrombocytopenia Syndrome Virus Antibodies in Individuals Living in an Endemic Area in Japan

et al. 2018

View full text Add to dashboard Cite

SUMMARY:Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infection with a high mortality rate. It is caused by the SFTS virus (SFTSV) and is endemic in some areas in western Japan, including the Kagoshima prefecture. In the present study, healthy individuals living in this prefecture were examined to assess for anti-SFTSV seroprevalence. An initial study was performed using the serum samples collected from a total of 646 individuals living in Kagoshima. At the same time, a questionnaire was used to collect information (such as occupation and a history of tick bite). Enzyme-linked immunosorbent assay and indirect immunofluorescence assay were used for the screening. Finally, the seroprevalence of anti-SFTSV antibodies was confirmed using a neutralization assay. Only 2 (0.3%) out of 646 study participants were positive for anti-SFTSV antibodies. No significant difference was observed between individuals who are at a high or low risk of tick bite in terms of seropositivity. Next, a total of 1,000 serum samples collected from general blood donors by the Japanese Red Cross Kyushu Block Blood Center were tested. None of these samples tested positive for anti-SFTSV antibodies. These results suggest a low seroprevalence of anti-SFTSV antibodies in healthy individuals living in an endemic area in Japan.

show abstract

Section: Discussionmentioning

confidence: 99%

Low Seroprevalence of Severe Fever with Thrombocytopenia Syndrome Virus Antibodies in Individuals Living in an Endemic Area in Japan

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Dried samples were individually packed in transparent plastic bags and kept under refrigeration (between 4 and 8ЊC) until analysis. An IgM enzymelinked immunosorbent assay was performed according to the methods described by Rocha et al (2013) with minor modiÞcations. Brießy, polystyrene 96-well microplates were coated with an equal mixture of DENV 1Ð 4 recombinant E proteins (50 ng of each DENV antigen per well) and incubated at 4ЊC overnight.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Effectiveness of Mass Trapping With BG-Sentinel Traps for Dengue Vector Control: A Cluster Randomized Controlled Trial in Manaus, Brazil

et al. 2014

View full text Add to dashboard Cite

The objective of this study was to assess the effectiveness of BG-Sentinel (BGS) traps for mass trapping at the household level to control the dengue vector, Aedes aegypti (L.), in Manaus (Brazil) by performing a cluster randomized controlled trial. After an initial questionnaire and baseline monitoring, 6 out of 12 clusters were randomly allocated to the intervention arm, where participating premises received one BGS trap for mass trapping. The other six clusters did not receive traps and were considered as the control arm. Biweekly monitoring with BGS in both arms assessed the impact of mass trapping. At the end of the study, a serological survey was conducted and a second questionnaire was conducted in the intervention arm. Entomological monitoring indicated that mass trapping with BGS traps significantly reduced the abundance of adult female Ae. aegypti during the first five rainy months. In the subsequent dry season when the mosquito population was lower, no effect of mass trapping was observed. Fewer Ae. aegypti females were measured in the intervention arm during the next rainy period, but no significant difference between arms was observed. The serological survey revealed that in participating houses of mass trapping areas recent dengue infections were less common than in control areas, although this effect was not statistically significant. The majority of participants responded positively to questions concerning user satisfaction. Our results suggest that BGS traps are a promising tool which might be deployed as part of dengue control programs; however, further investigations and larger scale studies are necessary.

show abstract

“…Several dengue immunoenzymatic assays have been developed with different formats as alternatives to PRNT. Using ELISA is an attractive possibility as an alternative test for PRNT with comparable performance and better feasibility in the future [24]. The diagnostic criteria for recent dengue infection using paired annual serum sample may be different from the criteria used for acute dengue infection using acute and convalescent sera because the rise in IgG level may decline.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal Antibody-Based Enzyme Immunoassay for Epidemiological Studies of Asymptomatic Dengue Infection

Chatchen¹,

Limkittikul²,

Sriburin³

et al. 2016

AID

View full text Add to dashboard Cite

Dengue virus (DENV) is the world most prevalent mosquito-transmitted virus. The incidence of dengue infection has been increasing and most of the infected people are asymptomatic or have non specific febrile illness. Laboratory test is essential to confirm dengue infection in epidemiological studies. We developed an indirect ELISA test based on monoclonal immunoglobulin against all four DENV serotypes and evaluated the test for the diagnosis of asymptomatic dengue infection in paired annual serum samples. The indirect ELISA was found to have a sensitivity 87.5% and specificity 78.3% at optimized cut off. The results from indirect ELISA demonstrate an incidence of asymptomatic dengue infection from children aged 4 -11 years in Ratchaburi province, Thailand (2006-2009). These findings indicate that the indirect ELISA is suitable for serodiagnosis and seroepidemiological studies of asymptomatic dengue infection.

show abstract

Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection

Cited by 15 publications

References 42 publications

Low Seroprevalence of Severe Fever with Thrombocytopenia Syndrome Virus Antibodies in Individuals Living in an Endemic Area in Japan

Low Seroprevalence of Severe Fever with Thrombocytopenia Syndrome Virus Antibodies in Individuals Living in an Endemic Area in Japan

Evaluation of the Effectiveness of Mass Trapping With BG-Sentinel Traps for Dengue Vector Control: A Cluster Randomized Controlled Trial in Manaus, Brazil

Monoclonal Antibody-Based Enzyme Immunoassay for Epidemiological Studies of Asymptomatic Dengue Infection

Contact Info

Product

Resources

About