Recombinant DNA methods for the oxytetracycline producer <i>Streptomyces rimosus</i>

Pm, Rhodes; Is, Hunter; Ej, Friend; Warren, M.J.

doi:10.1042/bst0120586

Cited by 46 publications

(16 citation statements)

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“…During the last 25 years of research into the genetics of S. rimosus, procedures for in vivo and in vitro genetic manipulations have been developed (201). These include protoplast preparation and regeneration and protoplast-mediated genetic exchange via fusion (62,105,108,163,222,223,228,229).…”

Section: Introductionmentioning

confidence: 99%

“…In the early 1970s, studies following three avenues of research were initiated. These were the biochemical characterization of non-OTC-producing mutants and mapping of the OTC genes (180,200), characterization and persistence of S. rimosus phages (104,107,193,225), and genetic and physical characterization of circular and linear S. rimosus plasmids (31,41,72,80,201,212).…”

Section: Introductionmentioning

confidence: 99%

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Genetics ofStreptomyces rimosus, the Oxytetracycline Producer

Petković

Cullum

Hranueli

et al. 2006

Microbiol Mol Biol Rev

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101

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SUMMARY From a genetic standpoint, Streptomyces rimosus is arguably the best-characterized industrial streptomycete as the producer of oxytetracycline and other tetracycline antibiotics. Although resistance to these antibiotics has reduced their clinical use in recent years, tetracyclines have an increasing role in the treatment of emerging infections and noninfective diseases. Procedures for in vivo and in vitro genetic manipulations in S. rimosus have been developed since the 1950s and applied to study the genetic instability of S. rimosus strains and for the molecular cloning and characterization of genes involved in oxytetracycline biosynthesis. Recent advances in the methodology of genome sequencing bring the realistic prospect of obtaining the genome sequence of S. rimosus in the near term.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetics ofStreptomyces rimosus, the Oxytetracycline Producer

Petković

Cullum

Hranueli

et al. 2006

Microbiol Mol Biol Rev

Self Cite

101

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show abstract

“…Recent work on antibiotic-producing Streptomyces has revealed that some genes which encode resistance mechanisms may be linked to the antibiotic biosynthesis genes on the chromosome Distler & Piepersberg, 1985;Murakami et al, 1986;Ohnuki et al, 1985b;Rhodes et al, 1984;Stanzak et al, 1986). Therefore, another advantage of the cloning of TcR genes is their use as specific DNA probes to identify the gene clusters encoding the associated anti biotic biosynthesis pathways.…”

Section: Introductionmentioning

confidence: 99%

Cloning, Expression in Escherichia coli and Nucleotide Sequence of a Tetracycline-resistance Gene from Streptomyces rimosus

Reynes¹,

Calmels²,

Drocourt³

et al. 1988

Microbiology

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“…Antibiotic biosynthesis genes are generally clustered in limited regions in the genome, and the genes conferring resistance to the organism's own products are usually located in or near the gene clusters (Rhodes et a/., 1984;Ohnuki et al, 1985;Chater & Bruton, 1985;Malpartida & Hopwood, 1986;Murakami et al, 1986;Stanzak et al, 1986;Fishman et al, 1987). These facts seem to suggest a tight genetic linkage between antibiotic biosynthesis genes and the resistance genes during the evolution and the horizontal distribution of antibiotic biosynthesis systems.…”

Section: Discussionmentioning

confidence: 99%